Summary
The chromosome region 5q22 harbouring the putative gene associated with adenomatous polyposis coli (APC) was microdissected and microcloned from GTG-banded human metaphase chromosomes. In order to determine the precise regional localization of the microdissected material, we used polymerase chain reaction amplified microclones as a bulk-probe in nonradioactive chromosomal in situ suppression hybridization of human metaphase spreads. Specific in situ hybridization signals were obtained on the long arm of chromosome 5 in accordance with the chromosomal region excised for the cloning procedure. The application of this detection system should provide a rapid and powerful tool for analyzing patients with translocations or microdeletions of a given chromosome region.
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References
Bodmer WF, Bailey CJ, Bodmer J, Bussey HJR, Ellis A, Gorman P, Lucibello FC, Murday VA, Rider SH, Scambler P, Sheer D, Solomon E, Spurr NK (1987) Localization of the gene for familial adenomatous polyposis on chromosome 5. Nature 328:614–616
ISCN (1985) An international system for human cytogenetic nomenclature. Report of the standing committee on human cytogenetic nomenclature. Hamden DG, Klinger HP (eds) Published in collaboration with Cytogenet Cell Genet. Karger, Basel
Jauch A, Daumer C, Lichter P, Murken J, Schroeder-Kurth T, Cremer T (1990) Chromosomal in situ suppression hybridization of human gonosomes and autosomes and its use in clinical cytogenetics. Hum Genet 85:145–150
Leppert M, Dobbs M, Scambler P, O'Connell P, Nakamura Y, Stauffer D, Woodward S, Burt R, Hughes J, Gardner E, Lathrop M, Wasmuth J, Lalouel J-M, White R (1987) The gene for familial polyposis coli maps to the long arm of chromosome 5. Science 238:1411–1413
Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80:224–234
Lüdecke H-J, Senger G, Claussen U, Horsthemke B (1989) Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature 338:348–350
Lüdecke H-J, Senger G, Claussen U, Horsthemke B (1990) Contruction and characterization of band-specific DNA libraries. Hum Genet 84:512–516
Senger G, Lüdecke H-J, Horsthemke B, Claussen U (1990) Microdissection of banded human chromosomes. Hum Genet 84:507–511
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Trautmann, U., Leuteritz, G., Senger, G. et al. Detection of APC region-specific signals by nonisotopic chromosomal in situ suppression (CISS)-hybridization using a microdissection library as a probe. Hum Genet 87, 495–497 (1991). https://doi.org/10.1007/BF00197175
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DOI: https://doi.org/10.1007/BF00197175