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CRISPR/Cas Mediated Genome Editing for Improving Stress Resilience in Plants

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New Frontiers in Plant-Environment Interactions

Abstract

In nature, the plants have to face and fight both the abiotic (cold, heat, drought, salinity, and heavy metals) and biotic stressors (pathogens, including bacteria, fungi, and viruses and insect pests) with colossal effects respectively of 50% and 20% to 40% of global yield loss in agriculture. Clustered Regularly Interspaced Short Palindromic Repeat/Cas (CRISPR/Cas) system has emerged as a pioneering genome editing toolbox due to its simplicity, versatility and adequacy to accomplish transgene free gene manipulations. CRISPR/Cas can be easily programmed to target specific DNA sequences, and it is much quicker and extra efficient than previous gene-editing technologies. One of the most promising applications of CRISPR/Cas in plants is the creation of crops with improved resistance to pests, pathogens, and environmental stressors. In this chapter, we concentrate on understanding the molecular mechanisms of CRISPR/Cas genome editing system, types and applications of CRISPR/Cas for the refinement of stress-related traits in crop plants.

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Correspondence to Deepu Pandita .

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© 2023 The Author(s), under exclusive license to Springer Nature Switzerland AG

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Pandita, D. (2023). CRISPR/Cas Mediated Genome Editing for Improving Stress Resilience in Plants. In: Aftab, T. (eds) New Frontiers in Plant-Environment Interactions. Environmental Science and Engineering. Springer, Cham. https://doi.org/10.1007/978-3-031-43729-8_7

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