Abstract
Date palm is dioecious, characterized by unisexual flowers located on separate male or female trees. Dioecy has always been an issue in date palm breeding because the sex of the plant cannot be known until it reaches its reproductive stage, which takes 5‒9 years to first flowering. Hence, early sex identification can enhance breeding and genetic improvement success in date palm. The breeding programs help in the selection and identification of superior seedlings of important traits. However, the sex of dioecious plants is usually tedious to identify. Sex determination may not only enhance genetic resources but also gear up breeding programs. No doubt, substantial progress has been made in understanding mechanisms of sex identification in date palm using morphological, physiological, biochemical, and cytological procedures but the discrimination in male and female plants by these techniques is not reliable. Sexuality in plants is regulated by genetic, epigenetic, and physiological mechanisms, and the most specialized mechanism of sex determination is the sex chromosomes. Molecular markers have been applied accurately in date palm breeding programs to differentiate male and female seedlings, but the development of more reliable molecular markers is needed. Hence, this chapter is focused on an understanding of floral biology, genetics of sex, and methods applied for sex determination in date palm.
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References
AbdAlla MM, Abd El-Kawy AM (2010) Karyotype analysis for date palm (Phoenix dactylifera L) compared with tissue culture derived plants. New York Sci J 3(11):165–170
Ageez A, Madboly E (2011) Identification of male specific molecular markers in date palm Sewi cultivar. Egypt J Genet Cytol 40:201–214
Al-Ani B, Zaid A, Shabana H (2010) On the status of chromosomes of the date palm (Phoenix dactylifera L.). Acta Hort 882:253–268
Al-Juburi HJ, Al-Masry HH, Al-Banna M et al (2001) Effect of some growth regulators on some fruit characteristics and productivity of date palm trees (Phoenix dactylifera L.) II. Khaniezy cultivar. In: Zaid A, Hegarty V, Al Kaabi HHS (eds) Proceedings of the second international conference on date palms, United Arab Emirates University, Al-Ain, UAE, pp 21–35
Al-Mahmoud ME, Al-Dous EK, Al-Azwani EK et al (2012) DNA-based assays to distinguish date palm (Arecaceae) gender. Amer J Bot 99(1):7–10
Atia MA, Adawy SS, El-Itriby HA (2017a) Date palm sex differentiation based on fluorescence in situ hybridization (FISH). In: Al-Khayri JM, Jain SM, Johnson DV (eds) Date palm biotechnology protocols, vol 2. Humana Press, New York, pp 245–256
Atia MA, Sakr MM, Mokhtar MM et al (2017b) Development of sex-specific PCR-based, markers in date palm. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Date palm biotechnology protocols, vol 2. Humana Press, New York, pp 227–244
Awan FS, Maryam Jaskani MJ et al (2017) Gender identification in date palm using molecular markers. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Date palm biotechnology protocols, vol 2. Humana Press, New York, pp 209–225
Ballardini M, Mercuri A, Littardi C et al (2013) The chloroplast DNA locus psbZ-trnfM as a potential barcode marker in Phoenix L. (Arecaceae). ZooKeys 365:71–82
Beach JH (1981) Pollinator foraging and the evolution of dioecy. Amer Nat 118:572–577
Bekheet SA, Hanafy MS (2011) Towards sex determination of date palm. In: Jain SM, Al-Khayri JM, Johnson DV (eds) Date palm biotechnology. Springer, New York, pp 551–566
Bekheet SA, Taha HS, Hanafy MS et al (2008) Morphogenesis of sexual embryos of date palm cultured in vitro and early identification of sex type. J Appl Sci Res 4:345–352
Chao CT, Krueger RR (2007) The date palm (Phoenix dactylifera L.): overview of biology, uses, and cultivation. HortScience 42(5):1077–1082
Cherif E, Zehdi S, Castillo K et al (2013) Male-specific DNA markers provide genetic evidence of an XY chromosome system, a recombination arrest and allow the tracing of paternal lineages in date palm. New Phytol 197(2):409–415
Danilova TV, Karlov GI (2006) Application of inter simple sequence repeat (ISSR) polymorphism for detection of sex-specific molecular markers in hop (Humulus lupulus L.). Euphytica 151(1):15–21
DeMason DA, Tisserat B (1980) The occurrence and structure of apparently bisexual flowers in the date palm, Phoenix dactylifera L. (Arecaceae). Bot J Linn Soc 81(4):283–292
Dhawan C, Kharb P, Sharma R et al (2013). Development of male-specific SCAR marker in date palm (Phoenix dactylifera L.). Tree Genet Genomes 9(5):1143–1150
Diggle PK, Di-Stilio VS, Gschwend AR et al (2011) Multiple developmental processes underlie sex differentiation in angiosperms. Trends Genet 27(9):368–376
Dransfield J, Uhl NW, Asmussen CB et al (2008) Genera palmarum: the evolution and classification of palms. Royal Botanic Gardens Kew, UK
El-Khishin DA, Adawy SS, Hussein EH et al (2003) AFLP fingerprinting of some Egyptian date palm (Phoenix dactylifera L.) cultivars. Arab J Biotech 6(2):223–234
Elmeer K, Mattat I (2012) Marker-assisted sex differentiation in date palm using simple sequence repeats. 3 Biotech 2(3):241–247
El-Yazal MA (2008) Sex determination of date palm (Phoenix dactylifera L.) through chemical composition of leaves. Fayoum J Agric Res Dev 22(2):76–87
Fakir N, Muhammad A, Ali GM et al (2018) Utility of morphological features, chemical composition of fruit and chloroplast genes in date palm (Phoenix dactylifera L.) characterization. Sarhad J Agric 32(2):378–388
Fatima G, Khan IA, Jaskani MJ et al (2014) Ecological significance of root anatomy in date palm (Phoenix dactylifera) cultivars from diverse origins. Int J Agric Biol 16:795–800
Fu L, Wang Q, Zhang M et al (2020) Electrochemical sex determination of dioecious plants using polydopamine-functionalized graphene sheets. Front Chem 8:1–8
George M, Nair SS, Malini KA et al (2007) Finite size effects on the electrical properties of sol–gel synthesized CoFe2O4 powders: deviation from Maxwell-Wagner theory and evidence of surface polarization effects. J Phys Appl Phys 40(6):1593–1602
Gros-Balthazard M, Battesti V, Ivorra S et al (2020) On the necessity of combining ethnobotany and genetics to assess agrobiodiversity and its evolution in crops: a case study on date palms (Phoenix dactylifera L.) in Siwa Oasis. Egypt. Evol Appl 00:1–23
Guttman DS, Charlesworth D (1998) An X-linked gene with a degenerate Y-linked homologue in a dioecious plant. Nature 393(6682):263–266
Habib M (2014) Development of sequence characterizes amplified revisions (SCAR) markers in date palm. Thesis Dissertation, University of Agriculture, Faisalabad
Haider MS, Khan IA, Jaskani MJ et al (2014a) Biochemical attributes of dates at three maturation stages. Emir J Food Agric 26(11):953–962
Haider MS, Khan IA, Jaskani MJ et al (2015) Assessment of morphological attributes of date palm accessions of diverse agro-ecological origin. Pak J Bot 47(3):1143–1151
Haider MS, Khan IA, Jaskani MJ et al (2018) Pomological and biochemical profiling of date fruits (Phoenix dactylifera L.) during different fruit maturation phases. Pak J Bot 50(3):1069–1076
Haider MS, Khan IA, Naqvi SA et al (2013) Fruit developmental stages effects on biochemical attributes in date palm. Pak J Agric Sci 50(4):577–583
Haider MS, Khan IA, Naqvi SA et al (2014b) Flesh and fiber characterization at three different edible stages of date fruit development. Int J Food Nutr Sci 3(4):16–22
Hazzouri KM, Gros-Balthazard M, Flowers JM et al (2019) Genome-wide association mapping of date palm fruit traits. Nat Comm 10(1):1–14
Honys D, Twell D (2004) Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biol 5(11):1–13
Intha N, Chaiprasart P (2018) Sex determination in date palm (Phoenix dactylifera L.) by PCR based marker analysis. Sci Hortic 236:251–255
Islam SE (2017) Evaluation of date palm male types as pollinators for Zaghloul and Samany date palm cvs. grown in Qalyubia governorate. Mid East J Agric Res 6(4):1049–1056
Johnson MAT, Kenton AY, Bennett MD (1989) Voanioala gerardii has the highest known chromosome number in the monocotyledons. Genome 32:328–333
Juarez C, Banks JA (1998) Sex determination in plants. Curr Opin Plant Biol 1:68–72
Khanam S, Sham A, Bennetzen JL et al (2012) Analysis of molecular marker-based characterization and genetic variation in date palm (Phoenix dactylifera L.). Austr J Crop Sci 6(8):1236–1244
Kharb P, Mitra C (2017) Early sex identification in date palm by male-specific sequence characterized amplified region (SCAR) markers. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Date palm biotechnology protocols, vol 2. Humana Press, New York, pp 199–207
Khosla PK, Kumari A (2015) Methods of sex determination in dioecious angiospermous plants. Lakshya J Sci Manag 1:1–9
Korpelainen H, Virtanen V, Kostamo K et al (2008) Molecular evidence shows that the moss Rhytidiadelphus subpinnatus (Hylocomiaceae) is clearly distinct from R. squarrosus. Mol Phylogenetics Evol 48(1):372–376
Kumar S, Kumari R, Sharma V (2014) Genetics of dioecy and casual sex chromosomes in plants. J Genet 93(1):241–277
Lebel-Hardenack S, Grant SR (1997) Genetics of sex determination in flowering plants. Trends Plant Sci 2(4):130–136
Leitch IJ, Beaulieu JM, Chase MW et al (2010) Genome size dynamics and evolution in monocots. J Bot 2010: Article ID 862516, https://doi.org/10.1155/2010/862516
Makhlouf-Gafsi I, Mokni-Ghribi A, Bchir B et al (2016) Physico-chemical properties and amino acid profiles of sap from Tunisian date palm. Sci Agric 73:85–90
Maryam, Jaskani MJ, Awan FS et al (2016) Development of molecular method for sex identification in date palm (Phoenix dactylifera L.) plantlets using novel sex-linked microsatellite markers. 3 Biotech 6:1–7
Maryam Jaskani MJ, Fatima B et al (2015) Evaluation of pollen viability in date palm cultivars under different storage temperatures. Pak J Bot 47(1):377–381
Maryam Jaskani MJ, Naqvi SA (2017) Date palm pollen storage and viability. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Date palm biotechnology protocols, vol 2. Humana Press, New York, pp 3–14
Masmoudi-Allouche F, Châari-Rkhis A, Kriaa W et al (2009) In vitro hermaphrodism induction in date palm female flower. Plant Cell Rep 28(1):1–10
Mathew LS, Spannagl M, Al-Malki AGB et al (2014) A first genetic map of date palm (Phoenix dactylifera) reveals long-range genome structure conservation in the palms. BMC Genom 15(1):1–10
Milewicz M, Sawicki J (2013) Sex-linked markers in dioecious plants. Plant Omics J 6:144–149
Mitchell CH, Diggle PK (2005) The evolution of unisexual flowers: morphological and functional convergence results from diverse developmental transitions. Amer J Bot 92(7):1068–1076
Mohanty JN, Chand SK, Joshi RK (2019) Multiple micro-RNAs regulate the floral development and sex differentiation in the dioecious cucurbit Coccinia grandis (L.) Voigt. Plant Mol Biol Rep 37:111–128
Mohei EL, Mohasseb HAA, Al-Khateeb AA et al (2019) Identification and sequencing of Date-SRY gene: A novel tool for sex determination of date palm (Phoenix dactylifera L.). Saudi J Biol Sci 26(3):514–523
Naqvi SA, Khan IA, Pintaud JC et al (2015) Morphological characterization of Pakistani date palm (Phoenix dactylifera L.) genotypes. Pak J Agri Sci 52(3):645–650
Oster M, Eriksson O (2007) Sex ratio mediated pollen limitation in the dioecious herb Antennaria dioica. Ecosci 14:387–398
Otero-Arnaiz A, Oyama K (2001) Reproductive phenology, seed set and pollination in Chamaedorea alternans, an understorey dioecious palm in a rain forest in Mexico. J Trop Ecol 17:745–754
Othmani A, Collin M, Sellemi A et al (2017) First reported case of spontaneous hermaphrodism in female date palm (Phoenix dactylifera L.), cv. Alligue. J Hort Sci Biotech 92(4):376–388
Qasim M, Naqvi SA (2012) Dates: a fruit from Heaven. In: Manickavasagan A, Essa M, Sukumar E (eds) Dates: production, processing, food and medicinal values. CRC Press, USA, pp 341–350
Rao PVKJ, Das M, Das SK (2009) Changes in physical and thermo-physical properties of sugarcane, palmyra-palm and date-palm juices at different concentration of sugar. J Food Eng 90:559–566
Raza MK, Jaskani MJ, Naqvi SA et al (2020) Exploitation of phenotypic diversity in male accessions of date palm (Phoenix dactylifera) and its use in germplasm conservation. Int J Agric Biol 24:133–144
Renganayaki K, Jessup RW, Burson BL et al (2005) Identification of male-specific AFLP markers in dioecious Texas bluegrass. Crop Sci 45(6):2529–2539
Roser M (2000) DNA amounts and qualitative properties of nuclear genomes in palms (Arecaceae). In: Wilson KL, Morrison DA (eds) Monocots: systematics and evolution. CSIRO, Melbourne, Australia, pp 538–544
Royal Botanic Gardens Kew (1914) The sex of date palm seedlings. Bull Misc Info Kew 1914:159–162. https://doi.org/10.2307/411539
Schlessman MA, Vary LB, Munzinger J et al (2014) Incidence, correlates, and origins of dioecy in the island flora of New Caledonia. Int J Plant Sci 175:271–286
Selbi W, Day AJ, Rugg MS et al (2006) Overexpression of hyaluronan synthase 2 alters hyaluronan distribution and function in proximal tubular epithelial cells. J Am Soc Nephrol 17(6):1553–1567
Sharma A, Zinta G, Rana S et al (2010) Molecular identification of sex in Hippophae rhamnoides L. using isozyme and RAPD markers. Forest Stud China 12(2):62–66
Shirkot P, Sharma DR, Mohapatra T (2002) Molecular identification of sex in Actinidia deliciosa var. deliciosa by RAPD markers. Sci Hortic 94(1–2):33–39
Sidky RA, Eldawyati MM (2012) Proliferation of female inflorescence explants of date palm. Ann Agric Sci 57(2):161–165
Siljak-Yakovlev S, Cerbah M, Coulaud J et al (2002) Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies. Theor Appl Genet 104(3):505–512
Siljak-Yakovlev S, Cerbah M, Sarr A et al (1996) Chromosomal sex determination and heterochromatin structure in date palm. Sex Plant Reprod 9(3):127–132
Sonia DDS, Laurent CL, Cosette P et al (2013) The date palm (Phoenix dactylifera L.) leaf proteome: identification of a gender biomarker to screen male parents. Pak Orthodontic J 6(1):18–23
Suda J, Kyncl T, Jarolimova V (2005) Genome size variation in Macaronesia angiosperms: forty percent of the Canarian endemic flora completed. Plant Syst Evol 252(3–4):215–238
Tuskan G, Di-Fazio SP, Faivre-Rampant P et al (2012) The obscure events contributing to the evolution of an incipient sex chromosome in Populus: a retrospective working hypothesis. Tree Genet Genomes 8(3):559–571
Vamosi JC, Vamosi SM, Barrett SCH (2006) Sex in advertising: dioecy alters the net benefits of attractiveness in Sagittaria latifolia (Alismataceae). Proc Royal Soc B: Biol Sci 273:2401–2407
Voigt FA, Jung S, Farwig N et al (2005) Low fruit set in a dioecious tree: pollination ecology of Commiphora harveyi in South Africa. J Trop Ecol 21(2):179–188
Wang F, Li F, Wang J et al (2011) Genetic diversity of the selected 64 potato germplasms revealed by AFLP markers. Mol Plant Breed 2(4):22–29
Williams JG, Kubelik AR, Livak KJ et al (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acid Res 18:6531–6535
Wilson WG, Harder LD (2003) Reproductive uncertainty and the relative competitiveness of simultaneous hermaphroditism versus dioecy. Amer Nat 162:220–241
Xia J, Lu J, Wang ZX et al (2013) Pollen limitation and Allee effect related to population size and sex ratio in the endangered Ottelia acuminata (Hydrocharitaceae): Implications for conservation and reintroduction. Plant Biol 15:376–383
Xu WJ, Wang BW, Cui KM (2004) RAPD and SCAR markers linked to sex determination in Eucommia ulmoides Oliv. Euphytica 136(3):233–238
Yamamoto M (1938) Cultural conflicts and accommodations of the first and second generation Japanese. Social Process Hawaii 4:4–48
Yunus MF, Abd Aziz M, Kadir MA et al (2013) In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers. Turk J Biol 37(6):716–725
Zaid A, de-Wet PF (2002) Pollination and bunch management. Chapter 8 In: Zaid A (ed) Date palm cultivation. FAO, Rome
Zango O, Cherif E, Chabrillange N et al (2017) Genetic diversity of Southeastern Nigerien date palms reveals a secondary structure within Western populations. Tree Genet Genomes 13(4):75
Zango O, Rey H, Bakasso Y et al (2016) Local practices and knowledge associated with date palm cultivation in Southeastern Niger. Agric Sci 7:586–603
Zehdi-Azouzi S, Cherif E, Moussouni S et al (2015) Genetic structure of the date palm (Phoenix dactylifera) in the old world reveals a strong differentiation between eastern and western populations. Ann Bot 116(1):101–112
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Naqvi, S.A., Shafqat, W., Haider, M.S., Awan, F.S., Khan, I.A., Jaskani, M.J. (2021). Gender Determination of Date Palm. In: Al-Khayri, J.M., Jain, S.M., Johnson, D.V. (eds) The Date Palm Genome, Vol. 1. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-73746-7_7
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