Abstract
Hazardous waste containing wastewaters should be treated with efficient and economically feasible methods for sustainable water management. Adaptations of novel wastewater treatment methods are required to protect the environment and to provide a high level of health protection. Conventional methods need to be combined with advanced methods to remove the toxic contaminants from wastewater. Treatment of wastewater with enzymes has been shown to improve the treatment efficiency with reduced sludge volume and reduced odour. High cost and stability of the enzymes are major limitations for the implications of enzymes in wastewater treatment. Nanomaterials with an enzyme-like activity, which are called nanozymes, are emerging as potential alternatives for natural enzymes in wastewater treatment.
Nanozymes have been shown oxidase, peroxidase, superoxide dismutase and catalase enzymes like activity. Nanozymes are highly stable than natural enzymes and can exhibit the activity at a wide range of pH and temperatures. Production cost is less than that of natural enzymes, and nanozymes can be stored for longer periods. Multi functionalization and reusability are some of the important properties for wider applications of nanozymes in different types of wastewaters.
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Abbreviations
- ABTS:
-
2, 2′-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)
- BSA-Pt:
-
bovine serum albumin – platinum
- ELISA:
-
Enzyme-linked immunosorbent assay
- HRP:
-
Horseradish peroxidase
- Km:
-
Michaelis–Menten constant
- OPD:
-
o-phenylenediamine
- PANI:
-
Polyaniline
- SOD:
-
Superoxide dismutase
- TMB:
-
3,3′,5,5′-tetramethylbenzidine
References
Abu-Elsaoud AM, Abdel-Azeem AM (2020) Light, electromagnetic spectrum, and photostimulation of microorganisms with special reference to Chaetomium. In: Recent developments on Genus Chaetomium. Springer, Cham, pp 377–393
Aitken MD (1993) Waste treatment applications of enzymes: opportunities and obstacles. Chem Eng J 52(2):B49–B58
Aitken MD, Massey IJ, Chen T, Heck PE (1994) Characterization of reaction products from the enzyme catalyzed oxidation of phenolic pollutants. Water Res 28(9):1879–1889
Al-Saydeh SA, El-Naas MH, Zaidi SJ (2017) Copper removal from industrial wastewater: A comprehensive review. J Ind Eng Chem 56:35–44
Bohdziewicz J (1998) Biodegradation of phenol by enzymes from Pseudomonas sp. immobilized onto ultrafiltration membranes. Process Biochem 33(8):811–818
Bolong N, Ismail AF, Salim MR, Matsuura T (2009) A review of the effects of emerging contaminants in wastewater and options for their removal. Desalination 239(1–3):229–246
Burgess JE, Pletschke BI (2008) Hydrolytic enzymes in sewage sludge treatment: a mini-review. Water SA 34(3):343–350
Burton SG, Boshoff A, Edwards W, Rose PD (1998) Biotransformation of phenols using immobilized polyphenol oxidase. J Mol Catal B Enzym 5(1–4):411–416
Cai K, Lv Z, Chen K, Huang L, Wang J, Shao F et al (2013) Aqueous synthesis of porous platinum nanotubes at room temperature and their intrinsic peroxidase-like activity. Chem Commun 49(54):6024–6026
Capeness MJ, Echavarri-Bravo V, Horsfall LE (2019) Production of biogenic nanoparticles for the reduction of 4-nitrophenol and oxidative laccase-like reactions. Front Microbiol 10:997
Chen W, Chen J, Liu AL, Wang LM, Li GW, Lin XH (2011) Peroxidase-like activity of cupric oxide nanoparticle. ChemCatChem 3(7):1151–1154
Chen W, Li S, Wang J, Sun K, Si Y (2019) Metal and metal-oxide nanozymes: bioenzymatic characteristics, catalytic mechanism, and eco-environmental applications. Nanoscale 11(34):15783–15793
Chivukula M, Renganathan V (1995) Phenolic azo dye oxidation by laccase from Pyricularia oryzae. Appl Environ Microbiol 61(12):4374–4377
Dec J, Bollag JM (1994) Use of plant material for the decontamination of water polluted with phenols. Biotechnol Bioeng 44(9):1132–1139
Dhir B (2014) Potential of biological materials for removing heavy metals from wastewater. Environ Sci Pollut Res 21(3):1614–1627
Duran N, Esposito E (2000) Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Appl Catal B Environ 28(2):83–99
Edwards W, Bownes R, Leukes WD, Jacobs EP, Sanderson R, Rose PD, Burton SG (1999) A capillary membrane bioreactor using immobilized polyphenol oxidase for the removal of phenols from industrial effluents. Enzym Microb Technol 24(3–4):209–217
Fan J, Yin JJ, Ning B, Wu X, Hu Y, Ferrari M et al (2011) Direct evidence for catalase and peroxidase activities of ferritin–platinum nanoparticles. Biomaterials 32(6):1611–1618
Ferrer I, Dezotti M, Durán N (1991) Decolorization of Kraft effluent by free and immobilized lignin peroxidases and horseradish peroxidase. Biotechnol Lett 13(8):577–582
Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N et al (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2(9):577–583
Ghernaout D, Ghernaout B (2012) Sweep flocculation as a second form of charge neutralization—a review. Desalin Water Treat 44(1–3):15–28
Gianfreda L, Sannino F, Filazzola MT, Leonowicz A (1998) Catalytic behavior and detoxifying ability of a laccase from the fungal strain Cerrena unicolor. J Mol Catal B Enzym 4(1–2):13–23
Grabski AC, Burgess RR, Rasmussen JK, Coleman PL (1996) Immobilization of manganese peroxidase from Lentinula edodes on alkylaminated Emphaze (TM) AB 1 polymer for generation of Mn3+ as an oxidizing agent. Appl Biochem Biotechnol 60(1):1–17
Gramss G, Voigt KD, Kirsche B (1999) Oxidoreductase enzymes liberated by plant roots and their effects on soil humic material. Chemosphere 38(7):1481–1494
Grey R, Höfer C, Schlosser D (1998) Degradation of 2-chlorophenol and formation of 2-chloro-1, 4-benzoquinone by mycelia and cell-free crude culture liquids of Trametes versicolor in relation to extracellular laccase activity. J Basic Microbiol 38(5–6):371–382
He W, Wu X, Liu J, Hu X, Zhang K, Hou S et al (2010) Design of AgM bimetallic alloy nanostructures (M= Au, Pd, Pt) with tunable morphology and peroxidase-like activity. Chem Mater 22(9):2988–2994
He W, Jia H, Li X, Lei Y, Li J, Zhao H et al (2012) Understanding the formation of CuS concave superstructures with peroxidase-like activity. Nanoscale 4(11):3501–3506
He W, Zhou YT, Wamer WG, Hu X, Wu X, Zheng Z et al (2013) Intrinsic catalytic activity of Au nanoparticles with respect to hydrogen peroxide decomposition and superoxide scavenging. Biomaterials 34(3):765–773
Hofrichter M, Vares K, Scheibner K, Galkin S, Sipilä J, Hatakka A (1999) Mineralization and solubilization of synthetic lignin by manganese peroxidases from Nematoloma frowardii and Phlebia radiata. J Biotechnol 67(2–3):217–228
Hollender J, Hopp J, Dott W (1997) Degradation of 4-Chlorophenol via the meta cleavage pathway by Comamonas testosteroni JH5. Appl Environ Microbiol 63(11):4567–4572
Hu X, Saran A, Hou S, Wen T, Ji Y, Liu W et al (2013) Au@ PtAg core/shell nanorods: tailoring enzyme-like activities via alloying. RSC Adv 3(17):6095–6105
Huang Y, Ren J, Qu X (2019) Nanozymes: classification, catalytic mechanisms, activity regulation, and applications. Chem Rev 119(6):4357–4412
Islam MA, Morton DW, Johnson BB, Mainali B, Angove MJ (2018) Manganese oxides and their application to metal ion and contaminant removal from wastewater. J Water Process Eng 26:264–280
Jiao X, Song H, Zhao H, Bai W, Zhang L, Lv Y (2012) Well-redispersed ceria nanoparticles: promising peroxidase mimetics for H2O2 and glucose detection. Anal Methods 4(10):3261–3267
Johjima T, Itoh N, Kabuto M, Tokimura F, Nakagawa T, Wariishi H, Tanaka H (1999) Direct interaction of lignin and lignin peroxidase from Phanerochaete chrysosporium. Proc Natl Acad Sci 96(5):1989–1994
Karam J, Nicell JA (1997) Potential applications of enzymes in waste treatment. J Chem Technol Biotechnol 69(2):141–153
Kim JE, Wang CJJ, Bollag JM (1997) Interaction of reactive and inert chemicals in the presence of oxidoreductases: Reaction of the herbicide bentazon and its metabolites with humic monomers. Biodegradation 8(6):387–392
Kuo MY, Hsiao CF, Chiu YH, Lai TH, Fang MJ, Wu JY et al (2019) Au@ Cu2O core@ shell nanocrystals as dual-functional catalysts for sustainable environmental applications. Appl Catal B Environ 242:499–506
Lapworth DJ, Baran N, Stuart ME, Ward RS (2012) Emerging organic contaminants in groundwater: a review of sources, fate and occurrence. Environ Pollut 163:287–303
Li W, Chen B, Zhang H, Sun Y, Wang J, Zhang J, Fu Y (2015) BSA-stabilized Pt nanozyme for peroxidase mimetics and its application on colorimetric detection of mercury (II) ions. Biosens Bioelectron 66:251–258
Liang M, Yan X (2019) Nanozymes: from new concepts, mechanisms, and standards to applications. Acc Chem Res 52(8):2190–2200
Liu S, Lu F, Xing R, Zhu JJ (2011) Structural effects of Fe3O4 nanocrystals on peroxidase-like activity. Chem Eur J 17(2):620–625
Lu XF, Bian XJ, Li ZC, Chao DM, Wang C (2013) A facile strategy to decorate Cu 9 S 5 nanocrystals on polyaniline nanowires and their synergetic catalytic properties. Sci Rep 3:2955
Ma F, Zheng L, Chi Y (2008) Applications of biological flocculants (BFs) for coagulation treatment in water purification: turbidity elimination. Chem Biochem Eng Q 22(3):321–326
Ma M, Zhang Y, Gu N (2011) Peroxidase-like catalytic activity of cubic Pt nanocrystals. Colloids Surf A Physicochem Eng Asp 373(1–3):6–10
Machuca A, Aoyama H, Durán N (1999) Isolation and partial characterization of an extracellular low-molecular mass component with high Phenoloxidase activity from Thermoascus aurantiacus. Biochem Biophys Res Commun 256(1):20–26
Mansilla HD, Rodriguez J, Ferraz A, Duran N (1997) Biodegradation of acidolysis lignins from Chilean hardwoods by the ascomycete Chrysonilia sitophila. World J Microbiol Biotechnol 13(5):545–548
Mohapatra M, Anand S (2010) Synthesis and applications of nano-structured iron oxides/hydroxides–a review. Int J Eng Sci Technol 2(8):127–146
Mu J, Wang Y, Zhao M, Zhang L (2012) Intrinsic peroxidase-like activity and catalase-like activity of Co 3 O 4 nanoparticles. Chem Commun 48(19):2540–2542
Oturan MA, Aaron JJ (2014) Advanced oxidation processes in water/wastewater treatment: principles and applications. A review. Crit Rev Environ Sci Technol 44(23):2577–2641
Pickard MA, Kadima TA, Carmichael RD (1991) Chloroperoxidase, a peroxidase with potential. J Ind Microbiol 7(4):235–241
Prakash NB, Sockan V, Jayakaran P (2014) Waste water treatment by coagulation and flocculation. Int J Eng Sci Innov Technol 3(2):479–484
Qin T, Ma R, Yin Y, Miao X, Chen S, Fan K et al (2019) Catalytic inactivation of influenza virus by iron oxide nanozyme. Theranostics 9(23):6920
Qin L, Hu Y, Wei H (2020) Nanozymes: preparation and characterization. In: Yan (ed) Nanozymology. Springer, Singapore, pp 79–101
Rama R, Mougin C, Boyer FD, Kollmann A, Malosse C, Sigoillot JC (1998) Biotransformation of bezo [a] pyrene in bench scale reactor using laccase of Pycnoporus cinnabarinus. Biotechnol Lett 20(12):1101–1104
Rodriguez E, Pickard MA, Vazquez-Duhalt R (1999) Industrial dye decolorization by laccases from ligninolytic fungi. Curr Microbiol 38(1):27–32
Saby C, Luong JH (1998) A biosensor system for chlorophenols using chloroperoxidase and a glucose oxidase based amperometric electrode. Electroanalysis 10(1):7–11
Shen LH, Bao JF, Wang D, Wang YX, Chen ZW, Ren L et al (2013) One-step synthesis of monodisperse, water-soluble ultra-small Fe3 O4 nanoparticles for potential bio-application. Nanoscale 5(5):2133–2141
Shin HY, Park TJ, Kim MI (2015) Recent research trends and future prospects in nanozymes. J Nanomater 2015: 1–11Â
Siddique MH, St Pierre CC, Biswas N, Bewtra JK, Taylor KE (1993) Immobilized enzyme catalyzed removal of 4-chlorophenol from aqueous solution. Water Res 27(5):883–890
Stasinakis AS (2008) Use of selected advanced oxidation processes (AOPs) for wastewater treatment–a mini review. Global NEST J 10(3):376–385
Vernekar AA, Das T, Ghosh S, Mugesh G (2016) A remarkably efficient MnFe2O4-based oxidase nanozyme. Chem Asian J 11(1):72–76
Wan Y, Qi P, Zhang D, Wu J, Wang Y (2012) Manganese oxide nanowire-mediated enzyme-linked immunosorbent assay. Biosens Bioelectron 33(1):69–74
Wang W, Jiang X, Chen K (2012a) Iron phosphate microflowers as peroxidase mimic and superoxide dismutase mimic for biocatalysis and biosensing. Chem Commun 48(58):7289–7291
Wang S, Chen W, Liu AL, Hong L, Deng HH, Lin XH (2012b) Comparison of the peroxidase-like activity of unmodified, amino-modified, and citrate-capped gold nanoparticles. ChemPhysChem 13(5):1199–1204
Wang X, Liu J, Qu R, Wang Z, Huang Q (2017) The laccase-like reactivity of manganese oxide nanomaterials for pollutant conversion: rate analysis and cyclic voltammetry. Sci Rep 7(1):1–10
Wang Q, Wei H, Zhang Z, Wang E, Dong S (2018) Nanozyme: an emerging alternative to natural enzyme for biosensing and immunoassay. TrAC Trends Anal Chem 105:218–224
Wang J, Huang R, Qi W, Su R, Binks BP, He Z (2019) Construction of a bioinspired laccase-mimicking nanozyme for the degradation and detection of phenolic pollutants. Appl Catal B Environ 254:452–462
Wei H, Wang E (2013) Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Chem Soc Rev 42(14):6060–6093
Wu L, Wan G, Hu N, He Z, Shi S, Suo Y et al (2018) Synthesis of porous CoFe2O4 and its application as a peroxidase mimetic for colorimetric detection of H2O2 and organic pollutant degradation. Nano 8(7):451
Xu P, Zeng GM, Huang DL, Feng CL, Hu S, Zhao MH et al (2012) Use of iron oxide nanomaterials in wastewater treatment: a review. Sci Total Environ 424:1–10
Zhang Y, Tian J, Liu S, Wang L, Qin X, Lu W et al (2012) Novel application of CoFe layered double hydroxide nanoplates for colorimetric detection of H2O2 and glucose. Analyst 137(6):1325–1328
Zhang X, He S, Chen Z, Huang Y (2013) CoFe2O4 nanoparticles as oxidase mimic-mediated chemiluminescence of aqueous luminol for sulfite in white wines. J Agric Food Chem 61(4):840–847
Zhang S, Li H, Wang Z, Liu J, Zhang H, Wang B, Yang Z (2015) A strongly coupled Au/Fe3O4/GO hybrid material with enhanced nanozyme activity for highly sensitive colorimetric detection, and rapid and efficient removal of Hg2+ in aqueous solutions. Nanoscale 7(18):8495–8502
Zinicovscaia I (2016) Conventional methods of wastewater treatment. In: Cyanobacteria for bioremediation of wastewaters. Springer, Cham, pp 17–25
Acknowledgements
The author would like to thank Department of Biotechnology, Government of in India for financial support and ICAR-National Dairy Research Institute, Karnal, India, for providing lab space. The author would like to express his sincere thanks to Dr. A K Mohanty for his valuable suggestions.
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Yata, V.K. (2021). Applications of Nanozymes in Wastewater Treatment. In: Daima, H.K., PN, N., Lichtfouse, E. (eds) Nanozymes for Environmental Engineering. Environmental Chemistry for a Sustainable World, vol 63. Springer, Cham. https://doi.org/10.1007/978-3-030-68230-9_4
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