Advertisement

A genetic approach to analyze the critical role of oxygen in bacteroid metabolism

  • H. Hennecke
  • M. Bott
  • T. Ramseier
  • L. Thöny-Meyer
  • H.-M. Fischer
  • D. Anthamatten
  • I. Kullik
  • B. Thöny

Abstract

There are two essential processes in endosymbiotic bacteroids for which the ambient oxygen concentration is critical: respiration and N2 fixation. The apparently paradoxical requirements of respiration for oxygen supply and of nitrogenase for oxygen exclusion are met (i) by the induction of a respiratory chain with a high-affinity terminal oxidase that functions at nanomolar free oxygen, and (ii) by a sophisticated redox control system that allows nitrogenase gene expression only at these low O2 tensions. Our laboratory is engaged in studies aimed at elucidating the molecular genetic basis of both processes (i) and (ii) in the soybean root nodule symbiont, Bradyrhizobium japonicwn. This article summarizes the progress made recently.

Keywords

Terminal Oxidase nifA Gene Paracoccus Denitrificans Upstream Activator Sequence NifA Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Appleby, C.A. (1984) Ann.Rev.Plant Physiol. 35, 443–478.CrossRefGoogle Scholar
  2. 2.
    Baker, M.E. (1989) Mol.Endocrinol. 3, 881–884.CrossRefGoogle Scholar
  3. 3.
    Batut, J., Daveran-Mingot, M.-L., David, M., Jacobs, J., Garnerone, A.M. & Kahn, D. (1989) EMBO J. 8, 1279–1286.Google Scholar
  4. 4.
    Berry, E.A. & Trumpower, B.L. (1985) J.Biol.Chem. 260, 2458–2467.Google Scholar
  5. 5.
    David, M., Daveran, M.-L., Batut, J., Dedieu, A., Dommergue, O., Ghai, J., Hertig, C., Boistard, P. & Kahn, D. (1988) Cell 54, 671–683.CrossRefGoogle Scholar
  6. 6.
    Fischer, H.-M. & Hennecke, H. (1987) Mol.Gen.Genet. 209, 621–626.CrossRefGoogle Scholar
  7. 7.
    Fischer, H.-M., Bruderer, T. & Hennecke, H. (1988) Nucl.Acids Res. 16, 2207–2224.CrossRefGoogle Scholar
  8. 8.
    Fischer, H.-M., Fritsche, S., Herzog, B. & Hennecke, H. (1989) FEBS Lett. 255, 167–171.CrossRefGoogle Scholar
  9. 9.
    Gennis, R.B. (1987) FEMS Microbiol.Rev. 46, 387–399.CrossRefGoogle Scholar
  10. 10.
    Higgins, C.F., Gallagher, M.P., Hyde, S.C., Mimmack, M.L. & Pearce, S.R. (1990) Phil.Trans.Royal Soc.Lond. B 326, 353–365.CrossRefGoogle Scholar
  11. 11.
    Kullik, I., Hennecke, H. & Fischer, H.-M. (1989) Arch.Microbiol. 151, 191–197.CrossRefGoogle Scholar
  12. 12.
    O’Brian, M.R. & Maier, R.J. (1989) Biochim.Biophys.Acta 974, 229–246.CrossRefGoogle Scholar
  13. 13.
    Ramseier, T.M., Kaluza, B., Studer, D., Gloudemans, T., Bisseling, T., Jordan, P.M., Jones, R.M., Zuber, M. & Hennecke, H. (1989) Arch.Microbiol. 151, 203–212.CrossRefGoogle Scholar
  14. 14.
    Ratet, P., Pawlowski, K., Schell, J. & de Bruijn, F.J. (1989) Mol.Microbiol. 3, 825–838.CrossRefGoogle Scholar
  15. 15.
    Rossmann, M.G., Liljas, A., Branden, C.-I. & Banaszak, L.J. (1975) in The Enzymes Vol. XI, ed. Boyer, P.D. (Academic Press, New York), pp. 61–102.CrossRefGoogle Scholar
  16. 16.
    Thöny, B., Fischer, H.-M., Anthamatten, D., Bruderer, T. & Hennecke, H. (1987) Nucl.Acids Res. 15, 8479–8499.CrossRefGoogle Scholar
  17. 17.
    Thöny, B., Anthamatten, D. & Hennecke, H. (1989) J.Bacteriol. 171, 4162–4169.Google Scholar
  18. 18.
    Thöny-Meyer, L., Stax, D. & Hennecke, H. (1989) Cell 57, 683–697.CrossRefGoogle Scholar
  19. 19.
    Virts, E.L., Stanfield, S.W., Helinski, D.R. & Ditta, G.S. (1988) Proc.Natl.Acad.Sci. USA 85, 3062–3065.CrossRefGoogle Scholar

Copyright information

© Routledge, Chapman & Hall, Inc. 1990

Authors and Affiliations

  • H. Hennecke
    • 1
  • M. Bott
    • 1
  • T. Ramseier
    • 1
  • L. Thöny-Meyer
    • 1
  • H.-M. Fischer
    • 1
  • D. Anthamatten
    • 1
  • I. Kullik
    • 1
  • B. Thöny
    • 1
  1. 1.Mikrobiologisches Institut, Eidgenössische Technische HochschuleETH-ZentrumZürichSwitzerland

Personalised recommendations