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Monoclonal Antibody Labeling with Indium-111 and Gadolinium via DTPA Chelation on Selective and Nonselective Sites of the Antibody

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Radiolabeled Monoclonal Antibodies for Imaging and Therapy

Part of the book series: NATO ASI Series ((NSSA,volume 152))

Abstract

The recent development of hybridoma technology has allowed the production of large quantities of monoclonal antibodies with predefined specificity (1); this has resulted in a renewed interest in their use as immunodiagnostic reagents. Radiolabeled monoclonal antibodies are today widely used for specific localization of tumors and metastases (2–4). The success of immunoscintigraphy has generated interest in the application of monoclonal antibodies as specific carriers of paramagnetic agents for magnetic resonance imaging (MRI). MRI has certain advantages over immunoscintigraphy — no ionizing radiation is involved, and the spatial resolution is higher and equals that of computed tomography. Early investigators felt that differences in relaxation times between malignant tumor and normal tissue made contrast agents unnecessary. However, despite the intrinsic tissue contrast, the injection of contrast agents, e.g., gadolinium-diethylenetriaminepentaacet ic acid (Gd-DTPA) increases the sensitivity and specificity of MR imaging as demonstrated by several investigators (5–7). Of the available paramagnetic ions, gadolinium has the greatest effect on proton relaxation time. Its association with DTPA produces a compound that gives a marked reduction in the proton relaxation time in vitro and in vivo and produces minimal acute toxicity with imaging doses (8,9).

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Authors and Affiliations

  1. ORIS Industrie, Gif sur Yvette, France

    J. C. Saccavini, C. Curtet, J. Bohy & C. Tellier

  2. UER Medicine, U-211 INSERM, Nantes, France

    C. Curtet

  3. RMN et Radioactivite Chimique U A 472, Nantes, France

    C. Tellier

Authors
  1. J. C. Saccavini
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  2. C. Curtet
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  3. J. Bohy
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  4. C. Tellier
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Editor information

Editors and Affiliations

  1. Brookhaven National Laboratory, Upton, New York, USA

    Suresh C. Srivastava

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© 1988 Springer Science+Business Media New York

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Saccavini, J.C., Curtet, C., Bohy, J., Tellier, C. (1988). Monoclonal Antibody Labeling with Indium-111 and Gadolinium via DTPA Chelation on Selective and Nonselective Sites of the Antibody. In: Srivastava, S.C. (eds) Radiolabeled Monoclonal Antibodies for Imaging and Therapy. NATO ASI Series, vol 152. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5538-0_18

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  • DOI: https://doi.org/10.1007/978-1-4684-5538-0_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5540-3

  • Online ISBN: 978-1-4684-5538-0

  • eBook Packages: Springer Book Archive

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