Skip to main content
SpringerLink
Log in

New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells

  • Chapter
  • First Online:
Metallomics

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 1055))

Abstract

Single cells represent the basic building units of life, and thus their study is one the most important areas of research. However, classical analysis of biological cells eludes the investigation of cell-to-cell differences to obtain information about the intracellular distribution since it only provides information by averaging over a huge number of cells. For this reason, chemical analysis of single cells is an expanding area of research nowadays. In this context, metallomics research is going down to the single-cell level, where high-resolution high-sensitive analytical techniques are required. In this chapter, we present the latest developments and applications in the fields of single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS), mass cytometry, laser ablation (LA)-ICP-MS, nanoscale secondary ion mass spectrometry (nanoSIMS), and synchrotron X-ray fluorescence microscopy (SXRF) for single-cell analysis. Moreover, the capabilities and limitations of the current analytical techniques to unravel single-cell metabolomics as well as future perspectives in this field will be discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

ALOD:

Absolute limit of detection

AP-MALDI:

Atmospheric pressure matrix-assisted laser desorption ionization

CE:

Capillary electrophoresis

cryo-XT:

Cryo nanoscale X-ray tomography

CyTOF:

Cytometry by time-of-flight

ESI-MS:

Electrospray ionization mass spectrometry

fs-LI-O-TOFMS:

Femtosecond laser ionization orthogonal time-of-flight mass spectrometry

HECIS:

High-efficiency cell introduction system

HPCN:

High performance concentric nebulizer

ICP-AES :

Inductively coupled plasma atomic emission spectrometry

ICP-MS:

Inductively coupled plasma mass spectrometry

ICP-SFMS:

Inductively coupled plasma sector field mass spectrometry

ICP-TOFMS:

Inductively coupled plasma time-of-flight mass spectrometry

LADE:

Liquid-assisted droplet ejection

LA-ICP-MS :

Laser ablation inductively coupled plasma mass spectrometry

LB-HPCN:

Large-bore high performance concentric nebulizer

MALDI:

Matrix-assisted laser desorption ionization

μDG:

Micro-droplet generator

M-DIS:

Micro-droplet injection system

μFI:

Micro-flow Injection

μXRF:

Micro-X-ray fluorescence

NPs:

Nanoparticles

PBS:

Phosphate-buffered saline

PDMS:

Poly(dimethylsiloxane)

PFH:

Perfluorohexane

QDs:

Quantum dots

RR:

Ruthenium red

SC-ICP-MS:

Single cell inductively coupled plasma mass spectrometry

SIMS:

Secondary ion mass spectrometry

SNMS:

Secondary neutral mass spectrometry

SOD:

Superoxide dismutase

SP-ICP-MS:

Single particle inductively coupled plasma mass spectrometry

SR-nXRF:

Synchrotron radiation nano-X-ray fluorescence

SXRF :

Synchrotron X-ray fluorescence

TEM:

Transmission electron microscopy

TOF-SIMS:

Time-of-flight secondary ion mass spectrometry

References

  • Amable L, Stephan C, Smith S, Merrifield R (2017) An introduction to single cell ICP-MS analysis. Perkin Elmer, USA, pp 3–39

    Google Scholar 

  • Audinot JN, Georgantzopoulou A, Piret JP, Gutleb AC, Dowsett D, Migeon HN, Hoffmann L (2013) Identification and localization of nanoparticles in tissues by mass spectrometry. Surf Interface Anal 45:230–233

    Article  CAS  Google Scholar 

  • Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, Lou X, Pavlov S, Vorobiev S, Dick JE, Tanner SD (2009) Mass cytometry: a novel technique for real-time single cell multi-target immunoassay based on inductively coupled plasma time of flight mass spectrometry. Anal Chem 81:6813–6822

    Article  CAS  PubMed  Google Scholar 

  • Benninghoven A, Rudenauer FG, Werner HW (1987) Secondary ion mass spectrometry: basic concepts, instrumental aspects, applications and trends. Wiley-Interscience, New York

    Google Scholar 

  • Boxer SG, Kraft ML, Weber PK (2009) Advances in imaging secondary ion mass spectrometry for biological samples. Annu Rev Biophys 38:53–74

    Article  CAS  PubMed  Google Scholar 

  • Büchner T, Drescher D, Traub H, Schrade P, Bachmann S, Jakubowski N, Kneipp J (2014) Relating surface-enhanced Raman scattering signals of cells to gold nanoparticle aggregation as determined by LA-ICP-MS micromapping. Anal Bioanal Chem 406:7003–7014

    Article  CAS  PubMed  Google Scholar 

  • Ceko MJ, Hummitzsch K, Hatzirodos N, Bonner WM, Aitken JB, Russell DL, Lane M, Rodgers RJ, Harris HH (2015) X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function. Metallomics 7:71–82

    Article  CAS  PubMed  Google Scholar 

  • Comi TJ, Makurath MA, Philip MC, Rubakhin SS, Sweedler JV (2017) MALDI MS guided liquid microjunction extraction for capillary electrophoresis − electrospray ionization MS analysis of single pancreatic islet cells. Anal Chem 89:7765–7772

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Degueldre C, Favarger PY (2003) Colloid analysis by single particle inductively coupled plasma-mass spectroscopy: a feasibility study. Colloids Surfaces A Physicochem Eng Asp 217:137–142

    Article  CAS  Google Scholar 

  • Dong Y, Li B, Aharoni A (2016) More than pictures: when MS imaging meets histology. Trends Plant Sci 21:686–698

    Article  CAS  PubMed  Google Scholar 

  • Drescher D, Giesen C, Traub H, Panne U, Kneipp J, Jakubowski N (2012) Quantitative imaging of gold and silver nanoparticles in single eukaryotic cells by laser ablation ICP-MS. Anal Chem 84:9684–9688

    Article  CAS  PubMed  Google Scholar 

  • Drescher D, Zeise I, Traub H, Guttmann P, Seifert S, Büchner T, Jakubowski N, Schneider G, Kneipp J (2014) In situ characterization of SiO2 nanoparticle biointeractions using BrightSilica. Adv Funct Mater 24:3765–3775

    Article  CAS  Google Scholar 

  • Gao Y, Lin Y, Zhang B, Zou D, He M, Dong B, Hang W, Huang B (2013) Single-cell elemental analysis via high irradiance femtosecond laser ionization time-of-flight mass spectrometry. Anal Chem 85:4268–4272

    Article  CAS  PubMed  Google Scholar 

  • Groombridge AS, Miyashita S, Fujii S, Nagasawa K, Okahashi T, Ohata M, Umemura T, Takatsu A, Inagaki K, Chiba K (2013) High sensitive elemental analysis of single yeast cells (Saccharomyces cerevisiae) by time-resolved inductively-coupled plasma mass spectrometry using a high efficiency cell introduction system. Anal Sci 29:597–603

    Article  CAS  PubMed  Google Scholar 

  • Gundlach-Graham A, Günther D (2016) Toward faster and higher resolution LA-ICPMS imaging: on the co-evolution of la cell design and ICPMS instrumentation Young Investigators in Analytical and Bioanalytical Science. Anal Bioanal Chem 408:2687–2695

    Article  CAS  PubMed  Google Scholar 

  • Guo Y, Baumgart S, Stärk H-J, Harms H, Müller S (2017) Mass cytometry for detection of silver at the bacterial single cell level. Front Microbiol 8:1–9

    Google Scholar 

  • Haase A, Arlinghaus HF, Tentschert J, Jungnickel H, Graf P, Mantion A, Draude F, Galla S, Plendl J, Goetz ME, Masic A, Meier W, Thünemann AF, Taubert A, Luch A (2011) Application of laser postionization secondary neutral mass spectrometry/time-of-flight secondary ion mass spectrometry in nanotoxicology: visualization of nanosilver in human macrophages and cellular responses. ACS Nano 5:3059–3068

    Article  CAS  PubMed  Google Scholar 

  • Hagenhoff B, Breitenstein D, Tallarek E, Möllers R, Niehuis E, Sperber M, Goricnik B, Wegener J (2013) Detection of micro- and nano-particles in animal cells by ToF-SIMS 3D analysis. Surf Interface Anal 45:315–319

    Article  CAS  Google Scholar 

  • Haraguchi H (2004) Metallomics as integrated biometal science. J Anal At Spectrom 19:5–14

    Article  CAS  Google Scholar 

  • Haraguchi H (2017a) Metallomics: the history over the last decade and a future outlook. Metallomics 9:1001–1013

    Article  PubMed  Google Scholar 

  • Haraguchi H (2017b) Metallomics: integrated biometal science. In: Ogra Y, Hirata T (eds) Metallomics: recent analytical techniques and applications. Springer, Berlin, pp 3–39

    Chapter  Google Scholar 

  • Haraguchi H, Ishii A, Hasegawa T, Matsuura H, Umemura T (2008) Metallomics study on all-elements analysis of salmon egg cells and fractionation analysis of metals in cell cytoplasm. Pure Appl Chem 80:2595–2608

    Article  CAS  Google Scholar 

  • Ho K-S, Chan W-T (2010) Time-resolved ICP-MS measurement for single-cell analysis and on-line cytometry. J Anal At Spectrom 25:1114–1122

    Article  CAS  Google Scholar 

  • Isaure M-P, Huguet S, Meyer C, Castillo-michel H, Testemale D, Vantelon D, Saumitou-laprade P, Verbruggen N, Sarret G (2015) Evidence of various mechanisms of Cd sequestration in the hyperaccumulator Arabidopsis halleri, the non-accumulator Arabidopsis lyrata , and their progenies by combined synchrotron-based techniques. J Exp Bot 66:3201–3214

    Article  CAS  PubMed  Google Scholar 

  • Isaure M-P, Leyh B, Salomé M, Krauss G-J, Schaumlöffel D, Dobritzsch D (2017) The aquatic hyphomycete Heliscus lugdunensis protects its hyphae tip cells from cadmium: a micro X-ray fluorescence and X-ray absorption near edge structure spectroscopy study. Spectrochim Acta Part B At Spectrosc 137:85–92

    Article  CAS  Google Scholar 

  • Ishihara Y, Aida M, Nomura A, Miyahara H, Hokura A, Okino A (2015) Development of Desolvation system for single-cell analysis using droplet injection inductively coupled plasma atomic emission spectroscopy. Anal Sci 31:781–785

    Article  CAS  PubMed  Google Scholar 

  • Laborda F, Bolea E, Jiménez-Lamana J (2014) Single particle inductively coupled plasma mass spectrometry: a powerful tool for nanoanalysis. Anal Chem 86:2270–2278

    Article  CAS  PubMed  Google Scholar 

  • Leonardo T, Farhi E, Boisson A, Vial J, Cloetens P, Bohic S (2014) Determination of elemental distribution in green micro-algae using synchrotron radiation nano and quantitative imaging of silver and cobalt. Metallomics 6:316–329

    Article  CAS  PubMed  Google Scholar 

  • Li F, Armstrong DW, Houk RS (2005) Behavior of bacteria in the inductively coupled plasma: atomization and production of atomic ions for mass spectrometry. Anal Chem 77:1407–1413

    Article  CAS  PubMed  Google Scholar 

  • Malherbe J, Penen F, Isaure MP, Frank J, Hause G, Dobritzsch D, Gontier E, Horréard F, Hillion F, Schaumlöffel D (2016) A new radio frequency plasma oxygen primary ion source on Nano secondary ion mass spectrometry for improved lateral resolution and detection of electropositive elements at single cell level. Anal Chem 88:7130–7136

    Article  CAS  PubMed  Google Scholar 

  • Managh AJ, Edwards SL, Bushell A, Wood KJ, Geissler EK, Hutchinson JA, Hutchinson RW, Reid HJ, Sharp BL (2013) Single cell tracking of gadolinium labeled CD4+ T cells by laser ablation inductively coupled plasma mass spectrometry. Anal Chem 85:10627–10634

    Article  CAS  PubMed  Google Scholar 

  • Maret W (2016) The metals in the biological periodic system of the elements: concepts and conjectures. Int J Mol Sci 17:1–8

    Article  CAS  Google Scholar 

  • Mathieu E, Lai B, Chain F, Langella P, Bachelet M, Masliah J, Seksik P, Policar C (2017) A cell-penetrant manganese superoxide dismutase (MnSOD) mimic is able to complement MnSOD and exerts an Antiinflammatory effect on cellular and animal models of inflammatory bowel diseases. Inorg Chem 56:2545–2555

    Article  CAS  PubMed  Google Scholar 

  • McRae R, Lai B, Fahrni CJ (2013) Subcellular redistribution and mitotic inheritance of transition metals in proliferating mouse fibroblast cells. Metallomics 5:52–61

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miyashita S, Groombridge AS, Fujii S, Takatsu A, Chiba K, Inagaki K (2014a) Time-resolved ICP-MS measurement: a new method for elemental and multiparametric analysis of single cells. Anal Sci 30:219–224

    Article  CAS  PubMed  Google Scholar 

  • Miyashita S, Groombridge AS, Fujii S, Minoda A, Takatsu A, Hioki A, Chiba K, Inagaki K (2014b) Highly efficient single-cell analysis of microbial cells by time-resolved inductively coupled plasma mass spectrometry. J Anal At Spectrom 29:1598–1606

    Article  CAS  Google Scholar 

  • Miyashita S, Fujii S, Shigeta K, Inagaki K (2017) Single cell analysis by using ICP-MS. In: Ogra Y, Hirata T (eds) Metallomics: recent analytical techniques and applications. Springer, Berlin, pp 107–124

    Chapter  Google Scholar 

  • Mueller L, Traub H, Jakubowski N, Drescher D, Baranov VI, Kneipp J (2014) Trends in single-cell analysis by use of ICP-MS. Anal Bioanal Chem 406:6963–6977

    Article  CAS  PubMed  Google Scholar 

  • Murray KK, Seneviratne CA, Ghorai S (2016) High resolution laser mass spectrometry bioimaging. Methods 104:118–126

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nomizu T, Nakashima H, Hotta Y, Tanaka T, Kawaguchi H (1992) Simultaneous measurement of the elemental content and size of airborne particles by inductively coupled plasma emission spectrometry combined with the light-scattering method. Anal Sci 8:527–531

    Article  CAS  Google Scholar 

  • Nomizu T, Kaneco S, Tanaka T, Yamamoto T, Kawaguchi H (1993) Determination of Femto-gram amounts of zinc and lead in individual airborne particles by inductively coupled plasma mass spectrometry with direct air-sample introduction. Anal Sci 9:843–846

    Article  CAS  Google Scholar 

  • Nomizu T, Kaneco S, Tanaka T, Ito D, Kawaguchi H, Vallee BT (1994) Determination of calcium content in individual biological cells by inductively coupled plasma atomic emission spectrometry. Anal Chem 66:3000–3004

    Article  CAS  Google Scholar 

  • Nuñez J, Renslow R, Cliff JB, Anderton CR (2018) NanoSIMS for biological applications: current practices and analyses. Biointerphases 13:03B301

    Article  Google Scholar 

  • Passarelli MK, Ewing AG (2013) Single-cell imaging mass spectrometry. Curr Opin Chem Biol 17:854–859

    Article  CAS  PubMed  Google Scholar 

  • Penen F, Isaure MP, Dobritzsch D, Bertalan I, Castillo-Michel H, Proux O, Gontier E, Le Coustumer P, Schaumlöffel D (2017) Pools of cadmium in Chlamydomonas reinhardtii revealed by chemical imaging and XAS spectroscopy. Metallomics 9:910–923

    Article  CAS  PubMed  Google Scholar 

  • Pett-Ridge J, Weber PK (2012) NanoSIP: NanoSIMS applications for microbial biology. In: Navid A (ed) Microbial systems biology. Springer, Berlin, pp 375–408

    Chapter  Google Scholar 

  • Qin Z, Caruso JA, Lai B, Matusch A, Becker JS (2011) Trace metal imaging with high spatial resolution: applications in biomedicine. Metallomics 3:28–37

    Article  CAS  PubMed  Google Scholar 

  • Roschzttardtz H, Grillet L, Isaure M, Conéjéro G, Ortega R, Curie C, Mari S (2011) Plant cell nucleolus as a hot spot for iron. J Biol Chem 286:27863–27866

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Roudeau S, Carmona A, Perrin L, Ortega R (2014) Correlative organelle fluorescence microscopy and synchrotron X-ray chemical element imaging in single cells. Anal Bioanal Chem 406:6979–6991

    Article  CAS  PubMed  Google Scholar 

  • Rubakhin SS, Romanova EV, Nemes P, Sweedler JV (2011) Profiling metabolites and peptides in single cells. Nat Methods 8:S20–S29

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schaumlöffel D, Hutchinson R, Malherbe J, Le Coustumer P, Gontier E, Isaure M-P (2016) Novel methods for bioimaging including LA-ICPMS, NanoSIMS, TEM/X-EDS, SXRF. In: Michalke B (ed) Metallomics: analytical techniques and speciation methods. Wiley-VCH, Weinheim, pp 83–116

    Chapter  Google Scholar 

  • Schmid A, Kortmann H, Dittrich PS, Blank LM (2010) Chemical and biological single cell analysis. Curr Opin Biotechnol 21:12–20

    Article  CAS  PubMed  Google Scholar 

  • Schober Y, Guenther S, Spengler B, Römpp A (2012) Single cell matrix-assisted laser desorption/ionization mass spectrometry imaging. Anal Chem 84:6293–6297

    Article  CAS  PubMed  Google Scholar 

  • Shigeta K, Traub H, Panne U, Okino A, Rottmann L, Jakubowski N (2013a) Application of a micro-droplet generator for an ICP-sector field mass spectrometer – optimization and analytical characterization. J Anal At Spectrom 28:646–656

    Article  CAS  Google Scholar 

  • Shigeta K, Koellensperger G, Rampler E, Traub H, Rottmann L, Panne U, Okino A, Jakubowski N (2013b) Sample introduction of single selenized yeast cells (Saccharomyces cerevisiae) by micro droplet generation into an ICP-sector field mass spectrometer for label-free detection of trace elements. J Anal At Spectrom 28:637–645

    Article  CAS  Google Scholar 

  • Slaveykova VI, Guignard C, Eybe T, Migeon HN, Hoffmann L (2009) Dynamic NanoSIMS ion imaging of unicellular freshwater algae exposed to copper. Anal Bioanal Chem 393:583–589

    Article  CAS  PubMed  Google Scholar 

  • Smart KE, Smith JAC, Kilburn MR, Martin BGH, Hawes C, Grovenor CRM (2010) High-resolution elemental localization in vacuolate plant cells by nanoscale secondary ion mass spectrometry. Plant J 63:870–879

    Article  CAS  PubMed  Google Scholar 

  • Storms HA, Brown KF, Stein JD (1977) Evaluation of a cesium positive ion source for secondary ion mass spectrometry. Anal Chem 49:2023–2030

    Article  CAS  Google Scholar 

  • Szyrwiel L, Shimura M, Shirataki J, Matsuyama S, Matsunaga A, Setner B, Szewczuk Z, Yamauchi K, Chavatte L (2015) A novel branched TAT 47–57 peptide for selective Ni 2+ introduction into the human fibrosarcoma cell nucleus. Metallomics 7:1155–1162

    Article  CAS  PubMed  Google Scholar 

  • Tanner SD, Bandura DR, Ornatsky O, Baranov VI, Nitz M, Winnik MA (2008) Flow cytometer with mass spectrometer detection for massively multiplexed single-cell biomarker assay. Pure Appl Chem 80:2627–2641

    Article  CAS  Google Scholar 

  • Tsang CN, Ho KS, Sun H, Chan WT (2011) Tracking bismuth antiulcer drug uptake in single helicobacter pylori cells. J Am Chem Soc 133:7355–7357

    Article  CAS  PubMed  Google Scholar 

  • Týčová A, Ledvina V, Klepárník K (2017) Recent advances in CE-MS coupling: instrumentation, methodology, and applications. Electrophoresis 38:115–133

    Article  CAS  PubMed  Google Scholar 

  • Umemura T, Matsui Y, Sinnosuke S, Fukai T, Fujimori E, Kumata H, Aoki M (2017) Comprehensive element analysis of prokaryotic and eukaryotic cells as well as organelles by ICP-MS. In: Ogra Y, Hirata T (eds) Metallomics: recent analytical techniques and applications. Springer, Berlin, pp 219–237

    Chapter  Google Scholar 

  • Van Malderen SJM, Vergucht E, De Rijcke M, Janssen C, Vincze L, Vanhaecke F (2016a) Quantitative determination and subcellular imaging of cu in single cells via laser ablation-ICP-mass spectrometry using high-density microarray gelatin standards. Anal Chem 88:5783–5789

    Article  CAS  PubMed  Google Scholar 

  • Van Malderen SJM, Managh AJ, Sharp BL, Vanhaecke F (2016b) Recent developments in the design of rapid response cells for laser ablation-inductively coupled plasma-mass spectrometry and their impact on bioimaging applications. J Anal At Spectrom 31:423–439

    Article  CAS  Google Scholar 

  • Verboket PE, Borovinskaya O, Meyer N, Günther D, Dittrich PS (2014) A new microfluidics-based droplet dispenser for ICPMS. Anal Chem 86:6012–6018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Vergucht E, Brans T, Beunis F, Garrevoet J, De Rijcke M, Bauters S, Deruytter D, Vandegehuchte M, Van Nieuwenhove I, Janssen C, Burghammer M, Vincze L (2015) In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers. Sci Rep 5:9049

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang D, Bodovitz S (2010) Single cell analysis: the new frontier in “omics”. Trends Biotechnol 28:281–290

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang HAO, Grolimund D, Giesen C, Borca CN, Shaw-Stewart JRH, Bodenmiller B, Günther D (2013) Fast chemical imaging at high spatial resolution by laser ablation inductively coupled plasma mass spectrometry. Anal Chem 85:10107–10116

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Wang B, Wang M, Zheng L, Chen H, Chai Z, Zhao Y, Feng W (2015) Time-resolved ICP-MS analysis of mineral element contents and distribution patterns in single cells. Analyst 140:523–531

    Article  CAS  PubMed  Google Scholar 

  • Wang H, He M, Chen B, Hu B (2017a) Advances in ICP-MS-based techniques for trace elements and their species analysis in cells. J Anal At Spectrom 32:1650–1659

    Article  CAS  Google Scholar 

  • Wang H, Chen B, He M, Hu B (2017b) A facile droplet-Chip-time-resolved inductively coupled plasma mass spectrometry online system for determination of zinc in single cell. Anal Chem 89:4931–4938

    Article  CAS  PubMed  Google Scholar 

  • Wedlock LE, Kilburn MR, Cliff JB, Filgueira L, Saunders M, Berners-Price SJ (2011) Visualising gold inside tumour cells following treatment with an antitumour Au(I) complex. Metallomics 3:917–925

    Article  CAS  PubMed  Google Scholar 

  • Wolfe-Simon F, Switzer Blum J, Kulp TR, Gordon GW, Hoeft SE, Pett-Ridge J, Stolz JF, Webb SM, Weber PK, Davies PCW, Anbar AD, Oremland RS, Hille R, Lane TW, Morel FM, Wolfe-Simon F, Davies PCW, Anbar AD, Rosen BP, Baer CD, Edwards JO, Rieger PH, Oremland RS, Stolz JF, Hollibaugh JT, Blum JS, Bindi AB, Buzzelli J, Stolz JF, Oremland RS, Takeuchi M, Makino W, Cotner J, Sterner R, Elser J, Mandelstam J, Smith PG, Pickering IJ, Holbrook S, Dickerson R, Kim SH, Oremland RS, Stolz JF, Quillaguaman J, Delgado O, Mattiasson B, Hatti-Kaul R (2011) A bacterium that can grow by using arsenic instead of phosphorus. Science 332:1163–1166

    Article  CAS  PubMed  Google Scholar 

  • Zavalin A, Todd EM, Rawhouser PD, Yang J, Norris JL, Caprioli RM (2012) Direct imaging of single cells and tissue at sub-cellular spatial resolution using transmission geometry MALDI MS. J Mass Spectrom 47:1473–1481

    Article  CAS  PubMed  Google Scholar 

  • Zhao F, Moore KL, Lombi E, Zhu Y (2014) Imaging element distribution and speciation in plant cells. Trends Plant Sci 19:183–192

    Article  CAS  PubMed  Google Scholar 

  • Zheng L-N, Wang M, Wang B, Chen H-Q, Ouyang H, Zhao Y-L, Chai Z-F, Feng W-Y (2013) Determination of quantum dots in single cells by inductively coupled plasma mass spectrometry. Talanta 116:782–787

    Article  CAS  PubMed  Google Scholar 

  • Zheng L-N, Wang M, Zhao L-C, Sun B-Y, Wang B, Chen H-Q, Zhao Y-L, Chai Z-F, Feng W-Y (2015) Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry. Anal Bioanal Chem 407:2383–2391

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials (IPREM), UMR 5254, CNRS-UPPA, Pau, France

    Javier Jiménez-Lamana, Joanna Szpunar & Ryszard Łobinski

Authors
  1. Javier Jiménez-Lamana
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joanna Szpunar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ryszard Łobinski
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Javier Jiménez-Lamana .

Editor information

Editors and Affiliations

  1. Universidade Estadual de Campinas – Unicamp, Institute of Chemistry, São Paulo, Brazil

    Marco Aurélio Zezzi Arruda

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Jiménez-Lamana, J., Szpunar, J., Łobinski, R. (2018). New Frontiers of Metallomics: Elemental and Species-Specific Analysis and Imaging of Single Cells. In: Arruda, M. (eds) Metallomics. Advances in Experimental Medicine and Biology(), vol 1055. Springer, Cham. https://doi.org/10.1007/978-3-319-90143-5_10

Download citation

Publish with us

Policies and ethics

Search

Navigation