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Single Cell Analysis by Using ICP-MS

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Metallomics

Abstract

Single cell analysis by using inductively coupled plasma mass spectrometry (ICP-MS) has been attracting attention for investigating a cell-to-cell variation of content of elements and biomolecules. ICP-MS has a unique feature that can be applied to a highly sensitive elemental analysis for evaluating a cell-to-cell variance or cellular uptake of elements, drugs, and nanoparticles and a multiparametric analysis for characterizing a cell population based on multiparameters, like a flow cytometry (so-called mass cytometry). In addition, subcellular localization analysis including multiparametric imaging can be performed with the coupling of a laser ablation system. In this chapter, we describe an overview of single cell analysis by using ICP-MS and its future prospects, focusing on cytometric analysis and imaging analysis.

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Authors and Affiliations

  1. National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 3-9, Umezono1-1-1, Tsukuba, 305-8563, Japan

    Shin-ichi Miyashita, Shin-ichiro Fujii & Kazumi Inagaki

  2. Environmental Management Research Institute (EMRI), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba West, Onogawa16-1, Tsukuba, Ibaraki, 305-8569, Japan

    Kaori Shigeta

Authors
  1. Shin-ichi Miyashita
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  2. Shin-ichiro Fujii
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  3. Kaori Shigeta
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  4. Kazumi Inagaki
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Corresponding author

Correspondence to Kazumi Inagaki .

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Editors and Affiliations

  1. Graduate School of Pharmaceutical Science, Chiba University, Chiba, Japan

    Yasumitsu Ogra

  2. Graduate School of Science, The University of Tokyo, Tokyo, Japan

    Takafumi Hirata

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Miyashita, Si., Fujii, Si., Shigeta, K., Inagaki, K. (2017). Single Cell Analysis by Using ICP-MS. In: Ogra, Y., Hirata, T. (eds) Metallomics. Springer, Tokyo. https://doi.org/10.1007/978-4-431-56463-8_5

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