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Linear Megaplasmids Spreading in the Andean Resistome

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Microbial Ecosystems in Central Andes Extreme Environments

Abstract

High-altitude wetlands and lakes in the Argentinean Puna are extreme and pristine environments. Bacteria living in such habitats must adapt to extremely harsh conditions, such as oligotrophy, high ultraviolet radiation, and high arsenic concentrations, among others. Commonly, genetic traits that confer resistance to the above factors could be encoded in extrachromosomal elements. In actinobacteria isolated from these high-altitude habitats, screening for plasmids has revealed the presence of novel linear megaplasmids for the genera Micrococcus and Brevibacterium. These linear replicons belong to a class of elements called invertrons, which are characterized by terminal inverted repeats and terminal proteins covalently attached to the DNA 5′ ends. Nucleotide sequencing studies of these linear plasmids has shown the presence of genes required for the survival in these particular habitats.

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Acknowledgements

This work was partially supported by the Agencia Nacional de Promoción Científica y Tecnológica (Proyectos de Investigación Científica y Tecnológica (PICT) number 2014-1716) and by the Alexander von Humboldt Foundation.

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Authors and Affiliations

  1. Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria

    Martin Wagenknecht

  2. Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (LIMLA), Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), Centros Científico Tecnológicos (CCT), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Tucumán, Argentina

    María Florencia Pérez & Julián Rafael Dib

  3. Instituto de Microbiología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina

    Julián Rafael Dib

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  1. Martin Wagenknecht
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Correspondence to Julián Rafael Dib .

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  1. LIMLA-PROIMI-CONICET, Tucuman, Argentina

    María Eugenia Farías

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Wagenknecht, M., Pérez, M.F., Dib, J.R. (2020). Linear Megaplasmids Spreading in the Andean Resistome. In: Farías, M. (eds) Microbial Ecosystems in Central Andes Extreme Environments. Springer, Cham. https://doi.org/10.1007/978-3-030-36192-1_18

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