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Development of Flax (Linum usitatissimum L.) Mutant Populations for Forward and Reverse Genetics

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Genetics and Genomics of Linum

Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 23))

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Abstract

Mutagenesis has long been used to increase genetic diversity in different crop species including cultivated flax. In particular, ethyl methane sulfonate (EMS) has been used to create large mutant populations that can be screened using TILLING to identify mutations in specific genes via a reverse genetics approach. Additionally, forward genetics can be used to identify mutants showing interesting phenotypes. Such approaches have been used to identify flax mutants showing modifications in fiber cell wall structure and seed metabolism. The creation and characterization of flax mutant populations is a powerful strategy for gene discovery and for introducing genetic variability in this species that has a narrow genetic base.

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Acknowledgments

SH and SG gratefully acknowledge the support of the University of Lille, the Research Federation (Université de Lille, CNRS FR 3688, FRABio, Biochimie Structurale et Fonctionnelle des Assemblages Biomoléculaires), the Region “Hauts-de-France” (project ALIBIOTECH), and the French National Research Agency (ANR, project PT-Flax).

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Authors and Affiliations

  1. Université de Lille, CNRS, UMR 8576 – UGSF – Unité de Glycobiologie Structurale et Fonctionnelle, Lille, 5900, France

    Sébastien Grec & Simon Hawkins

  2. Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Orsay, France

    Marion Dalmais & Abdelhafid Bendahmane

  3. Bench Bio Pvt. Ltd, Vapi, India

    Manash Chatterjee

Authors
  1. Sébastien Grec
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  2. Marion Dalmais
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  3. Manash Chatterjee
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  4. Abdelhafid Bendahmane
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  5. Simon Hawkins
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Correspondence to Simon Hawkins .

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Editors and Affiliations

  1. Department of Biology, Case Western Reserve University, Cleveland, OH, USA

    Christopher A. Cullis

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Grec, S., Dalmais, M., Chatterjee, M., Bendahmane, A., Hawkins, S. (2019). Development of Flax (Linum usitatissimum L.) Mutant Populations for Forward and Reverse Genetics. In: Cullis, C. (eds) Genetics and Genomics of Linum. Plant Genetics and Genomics: Crops and Models, vol 23. Springer, Cham. https://doi.org/10.1007/978-3-030-23964-0_10

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