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TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops

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Abstract

TILLING (Targeting Induced Local Lesions in Genomes) by Sequencing (TbyS) refers to the application of high-throughput sequencing technologies to mutagenised TILLING populations as a tool for functional genomics. TbyS can be used to identify and characterise induced variation in genes (controlling traits of interest) within large mutant populations, and is a powerful approach for the study and harnessing of genetic variation in crop breeding programmes. The extension of existing TILLING platforms by TbyS will accelerate crop functional genomics studies, in concert with the rapid increase in genome editing capabilities and the number and quality of sequenced crop plant genomes. In this mini-review, we provide an overview of the growth of TbyS and its potential applications to crop molecular breeding.

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Acknowledgements

CS is supported by Science Foundation Ireland (SFI; grants 02/IN.1/B49 and 08/IN.1/B1931). AK acknowledges the support of an NUI Galway College of Science studentship and support from BenchBio Pvt. Ltd. and its staff.

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Authors and Affiliations

  1. Genetics and Biotechnology Lab, Plant & AgriBiosciences Research Centre (PABC), School of Natural Sciences, C306 Áras de Brún, National University of Ireland Galway, University Road, Galway, H91 REW4, Ireland

    Anishkumar P. K. Kumar, Peter C. McKeown, Peter Ryder, Galina Brychkova, Manash Chatterjee & Charles Spillane

  2. Bench Bio Pvt.Ltd, Vapi, India

    Anishkumar P. K. Kumar & Manash Chatterjee

  3. Unité de Recherche en Génomique Végétale (URGV), Évry, France

    Adnane Boualem & Abdelhafid Bendahmane

  4. Metabolic Biology Department, John Innes Centre, Norwich, UK

    Abhimanyu Sarkar

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  1. Anishkumar P. K. Kumar
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  2. Peter C. McKeown
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  3. Adnane Boualem
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  8. Manash Chatterjee
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  9. Charles Spillane
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Correspondence to Charles Spillane.

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Kumar, A.P.K., McKeown, P.C., Boualem, A. et al. TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops. Mol Breeding 37, 14 (2017). https://doi.org/10.1007/s11032-017-0620-1

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  • DOI: https://doi.org/10.1007/s11032-017-0620-1

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