Gene Delivery to Mammalian Cells

Volume 2: Viral Gene Transfer Techniques

  • William C. Heiser

Part of the Methods in Molecular Biology™ book series (MIMB, volume 246)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Delivery Using Adenoviruses

    1. Front Matter
      Pages 1-1
    2. Joanne T. Douglas
      Pages 3-14
    3. Miroslava Ogorelkova, Seyyed Mehdy Elahi, David Gagnon, Bernard Massie
      Pages 15-27
    4. Sheila Connelly, Christine Mech
      Pages 37-52
    5. Daniel J. Weiss
      Pages 53-68
    6. Joseph M. Alisky, Beverly L. Davidson
      Pages 91-120
    7. Manel Cascalló, Ramon Alemany
      Pages 121-138
    8. Laura Timares, Joanne T. Douglas, Bryan W. Tillman, Victor Krasnykh, David T. Curiel
      Pages 139-154
  3. Delivery Using Adeno-Associated Viruses

    1. Front Matter
      Pages 155-155
    2. Thomas M. Daly
      Pages 157-165
    3. Andrew Smith, Roy Collaco, James P. Trempe
      Pages 167-177
    4. Roland W. Herzog
      Pages 179-194
    5. Thomas M. Daly
      Pages 195-199
    6. Christine L. Halbert, A. Dusty Miller
      Pages 201-212
    7. Danny Chu, Patricia A. Thistlethwaite, Christopher C. Sullivan, Mirta S. Grifman, Matthew D. Weitzman
      Pages 213-224
    8. Marco A. Passini, Deborah J. Watson, John H. Wolfe
      Pages 225-236
    9. Selvarangan Ponnazhagan, Frank Hoover
      Pages 237-243

About this book


Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors, adenovirus, adeno-associated viruses, alphavirus, herpes simplex virus, baculovirus, and retrovirus. Many of these techniques have only been in practice for a few years and are still being refined and updated. Some are being used not only in basic science, but also in gene therapy applications. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and physical methods.
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.

Editors and affiliations

  • William C. Heiser
    • 1
  1. 1.Bio-Rad LaboratoriesHercules

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-095-3
  • Online ISBN 978-1-59259-650-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book