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Gene Delivery to Mammalian Cells pp 53–68Cite as

Delivery of DNA to Lung Airway Epithelium

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 246))

Abstract

Delivering exogenous DNA or genes directly to the lung airways offers a unique and appealing opportunity for specifically targeting gene expression to airway and alveolar epithelium. A large body of literature and experience supports the feasibility of this approach. However, airway-directed gene delivery is not as simple as was originally anticipated. The lung has evolved both physical and immunologic barriers that can hinder effective transduction of epithelial cells (1–3). Much current work in lung gene therapy is directed toward overcoming the inflammatory and immune responses provoked by gene-transfer vectors while simultaneously maximizing vector delivery and subsequent gene expression.

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Author information

Authors and Affiliations

  1. Pulmonary and Critical Care, Vermont Lung Center, University of Vermont College of Medicine, Burlington, VT

    Daniel J. Weiss

Authors
  1. Daniel J. Weiss
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Editor information

Editors and Affiliations

  1. Bio-Rad Laboratories, Hercules, CA

    William C. Heiser

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© 2004 Humana Press Inc.,Totowa ,NJ

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Cite this protocol

Weiss, D.J. (2004). Delivery of DNA to Lung Airway Epithelium. In: Heiser, W.C. (eds) Gene Delivery to Mammalian Cells. Methods in Molecular Biology™, vol 246. Humana Press. https://doi.org/10.1385/1-59259-650-9:53

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  • DOI: https://doi.org/10.1385/1-59259-650-9:53

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-095-3

  • Online ISBN: 978-1-59259-650-8

  • eBook Packages: Springer Protocols

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