Overview
- Biology of microRNAs for use as tools
- Implementation of these molecules to improve processes in the Biopharmceutical field
- Methods to implement these molecules in the Bioprocess setting
- Includes supplementary material: sn.pub/extras
- Includes supplementary material: sn.pub/extras
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About this book
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, Â miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
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Keywords
Table of contents (8 chapters)
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Front Matter
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Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: MicroRNAs as Tools in Biopharmaceutical Production
Editors: Niall Barron
DOI: https://doi.org/10.1007/978-94-007-5128-6
Publisher: Springer Dordrecht
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Science+Business Media Dordrecht 2012
Hardcover ISBN: 978-94-007-5127-9Published: 16 November 2012
Softcover ISBN: 978-94-007-9526-6Published: 14 December 2014
eBook ISBN: 978-94-007-5128-6Published: 15 November 2012
Edition Number: 1
Number of Pages: X, 126
Topics: Pharmaceutical Sciences/Technology, Genetic Engineering, Animal Genetics and Genomics