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Manipulating Levels of Specific MicroRNAs in Mammalian Cells

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MicroRNAs as Tools in Biopharmaceutical Production

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Abstract

In this chapter, we discuss why microRNA (miRNA) manipulation is of interest to cell line engineering, and review the various approaches used to alter miRNA levels. We review the design and use of both synthetic and expressed miRNAs and miRNA inhibitors, as well as targeted insertion and deletion.

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Author information

Authors and Affiliations

  1. Research Biotechnology, Sigma-Aldrich, 2909 Laclede Avenue, 63103, Saint Louis, MO, USA

    Carol Kreader Ph.D.

  2. Cell Sciences and Development, SAFC/Sigma-Aldrich, 2909 Laclede Avenue, 63103, Saint Louis, MO, USA

    Nan Lin Ph.D.

Authors
  1. Carol Kreader Ph.D.
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  2. Nan Lin Ph.D.
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Correspondence to Carol Kreader Ph.D. .

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Editors and Affiliations

  1. , National Institute for Cellular Biotechn, Dublin City University, Dublin, Ireland

    Niall Barron

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Kreader, C., Lin, N. (2012). Manipulating Levels of Specific MicroRNAs in Mammalian Cells. In: Barron, N. (eds) MicroRNAs as Tools in Biopharmaceutical Production. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5128-6_8

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