Skip to main content
  • Book
  • Open Access
  • © 2015

The Impact of Food Bioactives on Health

in vitro and ex vivo models

  • Important contribution to "food and health" research

  • Highlights the strengths and weaknesses of various assays

  • Covers what outputs/data can be generated

  • Includes supplementary material:

Buying options

Softcover Book USD 59.99
Price excludes VAT (USA)
Hardcover Book USD 59.99
Price excludes VAT (USA)

Table of contents (28 chapters)

  1. Front Matter

    Pages i-xvii
  2. Gastrointestinal Digestion Models, General Introduction

    1. Front Matter

      Pages 1-2
    2. Static Digestion Models: General Introduction

      • Amparo Alegría, Guadalupe Garcia-Llatas, Antonio Cilla
      Pages 3-12Open Access
    3. InfoGest Consensus Method

      • Alan Mackie, Neil Rigby
      Pages 13-22Open Access
    4. Approaches to Static Digestion Models

      • Alan Mackie, Neil Rigby, Adam Macierzanka, Balazs Bajka
      Pages 23-31Open Access
    5. Dynamic Digestion Models: General Introduction

      • Eva C. Thuenemann
      Pages 33-36Open Access
    6. The TNO Gastro-Intestinal Model (TIM)

      • Mans Minekus
      Pages 37-46Open Access
    7. Dynamic Gastric Model (DGM)

      • Eva C. Thuenemann, Giuseppina Mandalari, Gillian T. Rich, Richard M. Faulks
      Pages 47-59Open Access
    8. Human Gastric Simulator (Riddet Model)

      • Maria J. Ferrua, R. Paul Singh
      Pages 61-71Open Access
    9. The DIDGI® System

      • Olivia Ménard, Daniel Picque, Didier Dupont
      Pages 73-81Open Access
  3. General Introduction to Cells, Cell Lines and Cell Culture

    1. Front Matter

      Pages 83-93
    2. Epithelial Cell Models; General Introduction

      • Tor Lea
      Pages 95-102Open Access
    3. Caco-2 Cell Line

      • Tor Lea
      Pages 103-111Open Access
    4. HT29 Cell Line

      • Daniel Martínez-Maqueda, Beatriz Miralles, Isidra Recio
      Pages 113-124Open Access
    5. The IPEC-J2 Cell Line

      • Hans Vergauwen
      Pages 125-134Open Access
    6. Co-cultivation of Caco-2 and HT-29MTX

      • Charlotte R. Kleiveland
      Pages 135-140Open Access
  4. Innate and Adaptive Immune Cells: General Introduction

    1. Front Matter

      Pages 141-145
    2. THP-1 and U937 Cells

      • Wasaporn Chanput, Vera Peters, Harry Wichers
      Pages 147-159Open Access
    3. Peripheral Blood Mononuclear Cells

      • Charlotte R. Kleiveland
      Pages 161-167Open Access
    4. PBMC-Derived T Cells

      • Daniel Lozano-Ojalvo, Rosina López-Fandiño, Iván López-Expósito
      Pages 169-180Open Access

About this book

“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists…). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.


  • Assays
  • Ex vivo
  • Health
  • In vitro
  • food bioactive

Editors and Affiliations

  • TNO, Zeist, The Netherlands

    Kitty Verhoeckx

  • Cork, Ireland

    Paul Cotter

  • Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Madrid, Spain

    Iván López-Expósito

  • Norwegian University of Life Sciences, As, Norway

    Charlotte Kleiveland, Tor Lea

  • Institute of Food Research Norwich Research Park, Norwich, United Kingdom

    Alan Mackie

  • Universidad Autonoma de Madrid, Madrid, Spain

    Teresa Requena

  • Polish Academy of Sciences, Olsztyn, Poland

    Dominika Swiatecka

  • Wageningen University, Wageningen, The Netherlands

    Harry Wichers

Bibliographic Information

  • Book Title: The Impact of Food Bioactives on Health

  • Book Subtitle: in vitro and ex vivo models

  • Editors: Kitty Verhoeckx, Paul Cotter, Iván López-Expósito, Charlotte Kleiveland, Tor Lea, Alan Mackie, Teresa Requena, Dominika Swiatecka, Harry Wichers

  • DOI:

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: The Editor(s) (if applicable) and the Author(s) 2015

  • License: CC BY-NC

  • Hardcover ISBN: 978-3-319-15791-7Published: 19 May 2015

  • Softcover ISBN: 978-3-319-35366-1Published: 09 October 2016

  • eBook ISBN: 978-3-319-16104-4Published: 29 April 2015

  • Edition Number: 1

  • Number of Pages: XVII, 338

  • Number of Illustrations: 22 b/w illustrations, 35 illustrations in colour

  • Topics: Food Microbiology, Food Science, Human Physiology

Buying options

Softcover Book USD 59.99
Price excludes VAT (USA)
Hardcover Book USD 59.99
Price excludes VAT (USA)