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Carbohydrate Biotechnology Protocols

  • Book
  • © 1999

Overview

Editors:
  1. Christopher Bucke

    1. School of Biosciences, University of Westminster, London, UK

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Part of the book series: Methods in Biotechnology (MIBT, volume 10)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Introduction to Carbohydrate Biotechnology

    • Christopher Bucke
    Pages 1-5

  3. Production and Isolation of Xanthan Gum

    • Félix García-Ochoa, Victoria E. Santos, José A. Casas
    Pages 7-21

  4. Alginate from Azotobacter vinelandii

    • Francesca Clementi, Mauro Moresi, Eugenio Parente
    Pages 23-42

  5. Production of Schizophyllan

    • Udo Rau
    Pages 43-55

  6. Enzymatic Synthesis of Cellulose

    • Shiro Kobayashi, Shin-ichiro Shoda, Michael J. Donnelly, Stephen P. Church
    Pages 57-69

  7. Modification of Alginate Using Mannuronan C-5-Epimerases

    • Helga ErtesvÃ¥g, Gudmund SkjÃ¥k-Bræk
    Pages 71-78

  8. Viscosity Control of Guar Polysaccharide Solutions by Treatment with Galactose Oxidase and Catalase Enzymes

    • Michael J. Donnelly
    Pages 79-88

  9. The Production of Cyclodextrins Using CGTase from Bacillus macerans

    • Jacob A. Rendleman Jr.
    Pages 89-101

  10. Production of Microbial Glycolipids

    • Siegmund Lang
    Pages 103-118

  11. Partial Enzymatic Hydrolysis of Starch to Maltodextrins on the Laboratory Scale

    • Leon M. Marchal
    Pages 119-127

  12. The Production of α(1→2)-Terminated Glucooligosaccharides

    • Marguerite Dols, Vincent Monchois, Magali Remaud-Siméon, René-Marc Willemot, Pierre F. Monsan
    Pages 129-139

  13. Enzymatic Production of Fructooligosaccharides from Sucrose

    • Jong Won Yun, Seung Koo Song
    Pages 141-151

  14. Enzymatic Production of Inulooligosaccharides from Inulin

    • Jong Won Yun, Dong Hyun Kim
    Pages 153-163

  15. One-Pot Enzymatic Synthesis of Sialyl T-Epitope

    • Vladimír KÅ™en
    Pages 165-182

  16. Hydrolysis of Hemicelluloses Using Combinations of Xylanases and Feruloyl Esterases

    • Craig B. Faulds, Paul A. Kroon, Begoña Bartolomé, Gary Williamson
    Pages 183-195

  17. Enzymatic Depolymerization of Chitins and Chitosans

    • Riccardo A. A. Muzzarelli, Vesna Stanic, Viviana Ramos
    Pages 197-211

  18. Synthesis of Homo- and Hetero-Oligosaccharides from Underivatized Sugars Using Glycosidases

    • Christopher Bucke, John Packwood, Sony Suwasono, Robert A. Rastall
    Pages 213-219

  19. Use of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE®) in the Elucidation of N-Linked Oligosaccharide Structures

    • Harish P. M. Kumar, Patricia A. Denny, Paul C. Denny
    Pages 221-234

  20. Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides

    • Astrid Zervosen, Lothar Elling
    Pages 235-254
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About this book

We are in a phase of the evolution of biotechnology in which the true and potential commercial importance of carbohydrates is becoming appre- ated more fully. Progress in providing hard facts to establish the commercial value ofpolysaccharides and oligosaccharides is limited, as always, by lack of funding and by a relative shortage of skilled practitioners in the production and analysis of those materials. Carbohydrate science has a reputation, not unmerited, for technical difficulty owing to the structural similarity of the many monosaccharide monomers and the potential, and real, complexity of oligosaccharides and polysaccharides, particularly heterosaccharides conta- ing many different monomers. Modem analytical and synthetic methods, in many cases using enzyme technology, are beginning to allow this complexity to be unraveled. Carbohydrate Biotechnology Protocols is aimed at those newcomers who have an interest in the production and use of carbohydrate materials, but have shied away from involvement for lack of detailed descriptions of appropriate methods, including the type of practical hints that may be provided by those skilled in those methods, but that are rarely described in research papers. The majority of the contributions to this book conform to the established format of the Methods in Biotechnology series. They begin with the theoretical and c- mercial background to the method or group of methods, provide a list of the reagents and equipment required for the procedure, then give a detailed st- by-step description of how to carry out the protocol.

Reviews

"...The recommendable book is very useful for students to start experiment work and for scientists and practitioners in doing experimental tasks."-Nahrung

"...the individual chapters are well written by subject matter authorities, and provide useful insights to the original research literature. The bench manual format of the book perhaps makes it most useful to active researchers interested in the specific protocols selected for this volume, although a wealth of interesting information is found among the chapter introductions and notes."-ASM News

"Each chapter of the volume is armed with necessary literature references, as basic sources of knowledge. The contributions throughout the book are easily readable and understandable, this is why I recommend the book not only to experts in synthetic chemistry, biochemistry, fermentation biotechnology, and applied enzymology, but also to beginners and students who are new to the field of carbohydrate biotechnology.-Models of Chemistry

"The protocols are clearly arranged and begin with a short theoretical and commercial background of the method, the list of reagents and equipment and continue with a comprehensive step-by-step description and a set of notes. Such procedure saves time of the user, indicating quickly which problems are likely to arise. The recommendable book is very useful for students to start experimental work and for scientists and practitioners in doing experimental tasks."-Nahrung

"...a valuable resource to more-experienced practitioners who require alternative methodologies....this volume will prove a useful resource for researchers of graduate level and above, with an interest in investigating carbohydrate biochemistry as applied to the fields of biotechnology and pharmacology." - Tibtech

Editors and Affiliations

  • School of Biosciences, University of Westminster, London, UK

    Christopher Bucke

Bibliographic Information

  • Book Title: Carbohydrate Biotechnology Protocols

  • Editors: Christopher Bucke

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1007/978-1-59259-261-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • Hardcover ISBN: 978-0-89603-563-8Published: 23 July 1999

  • Softcover ISBN: 978-1-61737-074-8Published: 19 November 2010

  • eBook ISBN: 978-1-59259-261-6Published: 05 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XII, 337

  • Topics: Biochemistry, general

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