Table of contents
About this book
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
Protein purification Recombinant proteins Escherichia coli Structural biology High-throughput cloning Expression screening Solubility enhancement
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-6887-9
- Copyright Information Springer Science+Business Media LLC 2017
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-6885-5
- Online ISBN 978-1-4939-6887-9
- Series Print ISSN 1064-3745
- Series Online ISSN 1940-6029
- About this book