Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters
The major goal of any protein expression experiment is to combine the maximum production per cell of soluble protein with the highest possible cell density to most efficiently obtain high yields of protein. A large number of parameters can be optimized in these experiments, but one of the most interesting parameters that have a strong effect on both per cell productivity and cell density is the cellular growth media coupled to the expression induction process. Using specialized media and testing multiple induction conditions, it is possible to significantly enhance the production of heterologous proteins from E. coli.
Key wordsProtein expression Growth media Autoinduction IPTG Solubility
This work has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract HHSN261200800001E. The content of this chapter does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.