Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1586)

Abstract

The major goal of any protein expression experiment is to combine the maximum production per cell of soluble protein with the highest possible cell density to most efficiently obtain high yields of protein. A large number of parameters can be optimized in these experiments, but one of the most interesting parameters that have a strong effect on both per cell productivity and cell density is the cellular growth media coupled to the expression induction process. Using specialized media and testing multiple induction conditions, it is possible to significantly enhance the production of heterologous proteins from E. coli.

Key words

Protein expression Growth media Autoinduction IPTG Solubility 

Notes

Acknowledgments

This work has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract HHSN261200800001E. The content of this chapter does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Troy Taylor
    • 1
  • John-Paul Denson
    • 1
  • Dominic Esposito
    • 1
  1. 1.Protein Expression Laboratory, Cancer Research Technology ProgramFrederick National Laboratory for Cancer ResearchFrederickUSA

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