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High-Throughput Glycomics and Glycoproteomics

Methods and Protocols

  • Gordan Lauc
  • Manfred Wuhrer

Part of the Methods in Molecular Biology book series (MIMB, volume 1503)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jasminka Krištić, Gordan Lauc
    Pages 1-12
  3. Ivo Ugrina, Harry Campbell, Frano Vučković
    Pages 13-19
  4. David Falck, Bas C. Jansen, Noortje de Haan, Manfred Wuhrer
    Pages 31-47
  5. Barbara Adamczyk, Henning Stöckmann, Róisín O’Flaherty, Niclas G. Karlsson, Pauline M. Rudd
    Pages 97-108
  6. Kathrin Stavenhagen, Hannes Hinneburg, Daniel Kolarich, Manfred Wuhrer
    Pages 109-119
  7. Lauren D. Wu, L. Renee Ruhaak, Carlito B. Lebrilla
    Pages 121-129
  8. Hannes Hinneburg, Falko Schirmeister, Petra Korać, Daniel Kolarich
    Pages 131-145
  9. Stjepan Krešimir Kračun, Jonatan Ulrik Fangel, Maja Gro Rydahl, Henriette Lodberg Pedersen, Silvia Vidal-Melgosa, William George Tycho Willats
    Pages 147-165
  10. Alba Hykollari, Katharina Paschinger, Barbara Eckmair, Iain B. H. Wilson
    Pages 167-184
  11. Stephanie Holst, Gabi W. van Pelt, Wilma E. Mesker, Rob A. Tollenaar, Ana I. Belo, Irma van Die et al.
    Pages 185-196
  12. Tamara Pavić, Olga Gornik
    Pages 197-205
  13. Thomas Klarić, Ivan Gudelj
    Pages 207-216
  14. Anna Agakova, Frano Vučković, Lucija Klarić, Gordan Lauc, Felix Agakov
    Pages 217-233
  15. Frederique Lisacek, Julien Mariethoz, Davide Alocci, Pauline M. Rudd, Jodie L. Abrahams, Matthew P. Campbell et al.
    Pages 235-264
  16. Back Matter
    Pages 273-274

About this book

Introduction

This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. This book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughput with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, High-Throughput Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field.

Keywords

glycosylation glycans in eukaryotic life tandem mass spectrometric analysis of protein glycosylation glycans with fluorescent labeling capillary electrophoresis laser induced fluorescence detection brain tissue in vitro cell culture milk oligosaccharides invertebrate and protist N-glycans

Editors and affiliations

  • Gordan Lauc
    • 1
  • Manfred Wuhrer
    • 2
  1. 1.Faculty of Pharmacy and BiochemistryUniversity of ZagrebZagrebCroatia
  2. 2.Center for Proteomics and MetabolomicsLeiden University Medical CenterLeidenThe Netherlands

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6493-2
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6491-8
  • Online ISBN 978-1-4939-6493-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site