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Hepatocytes

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Naoki Tanimizu

    1. Research Institute for Frontier Medicine, Sapporo Medical University, School of Medicine, Sapporo, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2544)

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About this book

This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Long-Term Expansion of Murine Primary Hepatocyte Organoids

    • Thomas A. Kluiver, Lianne J. Kraaier, Weng Chuan Peng
    Pages 1-13

  3. Generation of In Vivo Traceable Hepatocyte-Like Cells from Human iPSCs

    • Candice Ashmore-Harris, Gilbert O. Fruhwirth
    Pages 15-49

  4. Generation of Hepatocyte Organoids from Human iPS Cells

    • Giuseppe Pettinato
    Pages 51-70

  5. Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells

    • Lavinija Matakovic, Arend W. Overeem, Karin Klappe, Sven C. D. van IJzendoorn
    Pages 71-82

  6. Human Hepatocyte Transduction with Adeno-Associated Virus Vector

    • Zhenwei Song, Wenwei Shao, Liujiang Song, Xieolei Pei, Chengwen Li
    Pages 83-93

  7. Hepatocyte-Directed Delivery of Lipid-Encapsulated Small Interfering RNA

    • Laura Morán, Marius Maximilian Woitok, Matthias Bartneck, Francisco Javier Cubero
    Pages 95-106

  8. Co-culture Model for Hepatitis B Virus Infection Using iPSC-Derived Liver Progenitor Cells and Liver Sinusoidal Endothelial Cells

    • Misao Himeno, Shin-Wei Chen, Taketomo Kido
    Pages 107-117

  9. In Vitro Assay System to Detect Drug-Induced Bile Acid-Dependent Cytotoxicity Using Hepatocytes

    • Akinori Takemura, Kousei Ito
    Pages 119-127

  10. Evaluation of Urea Cycle Activity by Metabolic Flux Analysis Using Mass Spectrometry

    • Yasuharu Ueno, Takuji Maeda, Satoshi Okamoto, Hideki Taniguchi
    Pages 129-144

  11. Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes

    • Francisco Lázaro-Diéguez, Anne Müsch
    Pages 145-157

  12. Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations

    • Dong Fu
    Pages 159-169

  13. Assessment of Hepatocyte Ploidy by Flow Cytometry

    • Yinhua Jin, Teni Anbarchian, Roel Nusse
    Pages 171-181

  14. Isolation of Small Hepatocyte-Like Progenitor Cells from Retrorsine/Partial Hepatectomy Rat Livers by Laser Microdissection

    • Norihisa Ichinohe, Naoki Tanimizu, Toshihiro Mitaka
    Pages 183-193

  15. Application of EdU-Based DNA Synthesis Assay to Measure Hepatocyte Proliferation In Situ During Liver Regeneration

    • Amit Ghosh, Awais Ullah Ihsan, Madhuparna Nandi, Maryse Cloutier, Md Gulam Musawwir Khan, Sheela Ramanathan et al.
    Pages 195-206

  16. Quantification of Clonal Expansion of Hepatocytes in Normal and Injured Liver

    • Feng Chen
    Pages 207-216

  17. Evaluation of SOX9-Positive Hepatocytes in Human Liver Specimens and Mature Mouse Hepatocytes

    • Yuji Suzuki, Tokio Sasaki, Keisuke Kakisaka, Hiroaki Abe, Yasuhiro Takikawa
    Pages 217-225

  18. Identification of Genes Regulating Hepatocyte Injury by a Genome-Wide CRISPR-Cas9 Screen

    • Katherine Shortt, Daniel P. Heruth
    Pages 227-251

  19. Identification of Subtypes of HCC Using Bioinformatics and the Hepatocyte Differentiation Model

    • Wei Cheng, Yan Zhang, Ming Liu
    Pages 253-258

  20. 3D Culture of Primary Patient-Derived Hepatoblastoma Tumoroids

    • Peng V. Wu, Roel Nusse
    Pages 259-267
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Editors and Affiliations

  • Research Institute for Frontier Medicine, Sapporo Medical University, School of Medicine, Sapporo, Japan

    Naoki Tanimizu

Bibliographic Information

  • Book Title: Hepatocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Naoki Tanimizu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2557-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2556-9Published: 21 September 2022

  • Softcover ISBN: 978-1-0716-2559-0Published: 22 September 2023

  • eBook ISBN: 978-1-0716-2557-6Published: 20 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 281

  • Number of Illustrations: 7 b/w illustrations, 42 illustrations in colour

  • Topics: Physiology, Biomedicine, general

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