Abstract
The in vitro maintenance and expansion of primary hepatocytes provide immense opportunities for disease modeling and other biological, viral, and toxicological studies, as well as for applications in regenerative medicine such as cell transplantation for the treatment of metabolic liver diseases. Here, we describe a protocol for the isolation and in vitro culture of primary hepatocytes in a three-dimensional extracellular matrix gel. The inflammatory cytokine tumor necrosis factor alpha (TNFα) is crucial for the long-term expansion of mouse hepatocyte organoids by mimicking the regenerative response in vitro. Long-term cultured hepatocyte organoids express high levels of hepatocyte markers upon differentiation.
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Change history
19 November 2022
In the original version of this book, Figure 1 of Chapter 1 has been published incorrectly. This has been rectified in the updated version of this book.
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Acknowledgments
We are grateful to the members of the Nusse lab (Stanford University) and Ziqin Tang (Princess Máxima Center for Pediatric Oncology) for providing materials and assistance for this manuscript.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Kluiver, T.A., Kraaier, L.J., Peng, W.C. (2022). Long-Term Expansion of Murine Primary Hepatocyte Organoids. In: Tanimizu, N. (eds) Hepatocytes. Methods in Molecular Biology, vol 2544. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2557-6_1
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DOI: https://doi.org/10.1007/978-1-0716-2557-6_1
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2556-9
Online ISBN: 978-1-0716-2557-6
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