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  • Open Access
  • © 2022

Whole-Body Regeneration

Methods and Protocols

  • This book is open access, which means that you have free and unlimited access

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2450)

Buying options

Softcover Book USD 49.99
Price excludes VAT (USA)
Hardcover Book USD 59.99
Price excludes VAT (USA)

Table of contents (37 protocols)

  1. Front Matter

    Pages i-xvii
  2. Historical Overviews

    1. Front Matter

      Pages 1-1
    2. The Hazards of Regeneration: From Morgan’s Legacy to Evo-Devo

      • Chiara Sinigaglia, Alexandre Alié, Stefano Tiozzo
      Pages 3-25Open Access
    3. Studying Regeneration in Ascidians: An Historical Overview

      • Virginia Vanni, Loriano Ballarin, Fabio Gasparini, Anna Peronato, Lucia Manni
      Pages 27-48Open Access
  3. Zoological Approaches

    1. Front Matter

      Pages 49-49
    2. Studying Protista WBR and Repair Using Physarum polycephalum

      • Megan M. Sperry, Nirosha J. Murugan, Michael Levin
      Pages 51-67Open Access
    3. Studying Porifera WBR Using the Calcerous Sponges Leucosolenia

      • Andrey I. Lavrov, Alexander V. Ereskovsky
      Pages 69-93Open Access
    4. Studying Ctenophora WBR Using Mnemiopsis leidyi

      • Julia Ramon-Mateu, Allison Edgar, Dorothy Mitchell, Mark Q. Martindale
      Pages 95-119Open Access
    5. Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerens

      • Hans-Jürgen Osigus, Michael Eitel, Karolin Horn, Kai Kamm, Jennifer Kosubek-Langer, Moritz Jonathan Schmidt et al.
      Pages 121-133Open Access
    6. Collecting and Culturing Kamptozoans for Regenerative Studies

      • Achim Meyer, Julia Merkel, Bernhard Lieb
      Pages 135-149Open Access
    7. Collecting and Culturing Bryozoans for Regenerative Studies

      • Abigail M. Smith, Peter B. Batson, Katerina Achilleos, Yuta Tamberg
      Pages 151-177Open Access
    8. Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride

      • Chiao-Ping Chen, Sheridan Ke-Wing Fok, Cheng-Yi Chen, Fei-Man Hsu, Yu-Wen Hsieh, Jiun-Hong Chen
      Pages 179-194Open Access
    9. Studying Annelida Regeneration Using Platynereis dumerilii

      • Michel Vervoort, Eve Gazave
      Pages 207-226Open Access
    10. Collecting and Culturing Lineus sanguineus to Study Nemertea WBR

      • Eduardo E. Zattara, Fernando A. Fernández-Alvarez
      Pages 227-243Open Access
    11. Studying Xenacoelomorpha WBR Using Isodiametra pulchra

      • Bernhard Egger
      Pages 245-261Open Access
    12. Studying Echinodermata Arm Explant Regeneration Using Echinaster sepositus

      • Cinzia Ferrario, Yousra Ben Khadra, Michela Sugni, M. Daniela Candia Carnevali, Pedro Martinez, Francesco Bonasoro
      Pages 263-291Open Access
    13. Studying Hemichordata WBR Using Ptychodera flava

      • Asuka Arimoto, Kuni Tagawa
      Pages 293-309Open Access
    14. Studying Tunicata WBR Using Botrylloides anceps

      • Arzu Karahan, Esra Öztürk, Berivan Temiz, Simon Blanchoud
      Pages 311-332Open Access
  4. Cellular Approaches

    1. Front Matter

      Pages 333-333

About this book

This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.


Keywords

  • immune response
  • cell sorting
  • stem cells
  • cell isolation
  • apoptosis
  • Open Access

Editors and Affiliations

  • Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon Blanchoud

  • Dept Génétique et Evolution, Université de Genève, Genève, Geneva, Switzerland

    Brigitte Galliot

Bibliographic Information

  • Book Title: Whole-Body Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Simon Blanchoud, Brigitte Galliot

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2172-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2022

  • License: CC BY

  • Hardcover ISBN: 978-1-0716-2171-4Published: 01 April 2022

  • Softcover ISBN: 978-1-0716-2174-5Published: 01 April 2022

  • eBook ISBN: 978-1-0716-2172-1Published: 31 March 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 685

  • Number of Illustrations: 8 b/w illustrations, 123 illustrations in colour

  • Topics: Regenerative Medicine and Tissue Engineering, Stem Cell Biology

Buying options

Softcover Book USD 49.99
Price excludes VAT (USA)
Hardcover Book USD 59.99
Price excludes VAT (USA)