Yeast Genetics

Methods and Protocols

  • Jeffrey S. Smith
  • Daniel J. Burke

Part of the Methods in Molecular Biology book series (MIMB, volume 1205)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mark Weir, Jill B. Keeney
    Pages 29-35
  3. Radhika Mathur, Peter Kaiser
    Pages 37-44
  4. Jennifer M. Gardner, Sue L. Jaspersen
    Pages 45-78
  5. Heimo Wolinski, Sepp D. Kohlwein
    Pages 91-109
  6. Elena Kuzmin, Sara Sharifpoor, Anastasia Baryshnikova, Michael Costanzo, Chad L. Myers, Brenda J. Andrews et al.
    Pages 143-168
  7. Namal V. C. Coorey, Liam D. P. Sampson, Jacqueline M. Barber, David S. Bellows
    Pages 169-186
  8. John Rodgers, Jingyu Guo, John L. Hartman IV
    Pages 187-208
  9. Maria D. Mayán, Luis Aragón
    Pages 219-229
  10. Philippe Lefrançois, Jennifer E. G. Gallagher, Michael Snyder
    Pages 231-255
  11. Lior David, Sandra Clauder-Münster, Lars M. Steinmetz
    Pages 257-273
  12. Andrew Miller, Ann L. Kirchmaier
    Pages 275-302
  13. Joseph M. Johnson, Jeffrey S. Smith, David A. Schneider
    Pages 303-328
  14. Sandra K. Villwock, Oscar M. Aparicio
    Pages 329-340
  15. George L. Sutphin, Joe R. Delaney, Matt Kaeberlein
    Pages 341-357
  16. Vincent R. Richard, Simon D. Bourque, Vladimir I. Titorenko
    Pages 359-373
  17. Back Matter
    Pages 375-378

About this book


Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome, and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered, and tagged at will. Saccharomyces cerevisiae, has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription, and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.


PCR mutagenesis cell imaging eukaryotic organism yeast genome yeast transformation

Editors and affiliations

  • Jeffrey S. Smith
    • 1
  • Daniel J. Burke
    • 2
  1. 1.Department of Biochemistry and Molecular GeneticsUniversity of Virginia School of MedicineCharlottesvilleUSA
  2. 2.Department of Biochemistry and Molecular GeneticsUniversity of Virginia School of MedicineCharlottesvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1362-6
  • Online ISBN 978-1-4939-1363-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site