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Single Yeast Cell Imaging

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Book cover Yeast Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1205))

Abstract

Microscopic imaging techniques play a pivotal role in the life sciences. Here we describe labeling and imaging methods for live yeast cell imaging. Yeast is an excellent reference organism for biomedical research to investigate fundamental cellular processes, and has gained great popularity also for large-scale imaging-based screens. Methods are described to label live yeast cells with organelle-specific fluorescent dyes or GFP-tagged proteins, and how cells are maintained viable over extended periods of time during microscopy. We point out common pitfalls and potential microscopy artifacts arising from inhomogeneous labeling and depending on cellular physiology. Application and limitation of bleaching techniques to address dynamic processes in the yeast cell are described.

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Acknowledgements

Work in the authors’ laboratories is supported by grants from the Austrian Science Funds FWF (project SFB Lipotox F3005 and the PhD program Molecular Enzymology W901-B12) and the Austrian Federal Ministry for Science and Research (project GOLD, in the framework of the Austrian Genomics Program, GEN-AU), and by NAWI Graz.

Author information

Authors and Affiliations

  1. Institute of Molecular Biosciences, University of Graz, Graz, Austria

    Heimo Wolinski

  2. Institute of Molecular Biosciences, BioTechMed Graz and BioImaging Graz, University of Graz, Humboldtstrasse 50/II, 8010, Graz, Austria

    Sepp D. Kohlwein

Authors
  1. Heimo Wolinski
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  2. Sepp D. Kohlwein
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Corresponding author

Correspondence to Sepp D. Kohlwein .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

    Jeffrey S. Smith

  2. Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA

    Daniel J. Burke

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© 2014 Springer Science+Business Media New York

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Wolinski, H., Kohlwein, S.D. (2014). Single Yeast Cell Imaging. In: Smith, J., Burke, D. (eds) Yeast Genetics. Methods in Molecular Biology, vol 1205. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1363-3_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1363-3_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1362-6

  • Online ISBN: 978-1-4939-1363-3

  • eBook Packages: Springer Protocols

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