Skip to main content
SpringerLink
Log in

MR images of mouse brain using clinical 3T MR scanner and 4CH-Mouse coil

  • Published:
Journal of the Korean Physical Society Aims and scope Submit manuscript

Abstract

Objectives: Although small-bore high-field magnets are useful for research in small rodent models,this technology, however, has not been easily accessible to most researchers. This current study, thus,tried to evaluate the usability of 4CH-Mouse coil (Philips Healthcare, Best, the Netherlands) forpreclinical investigations in clinical 3T MR scan environment. We evaluated the effects of ischemicpreconditioning (IP) in the mouse stroke model with clinical 3T MR scanner and 4CH-Mouse coil.

Materials and Methods: Experiments were performed on male C57BL/6 mice that either received the IP or sham operation (control). Three different MR sequences including diffusion weighted images (DWI), T2-weighted images (T2WI), and fluid attenuated inversion recovery (FLAIR) were performed on the mouse brains following 24, 72 hours of middle cerebral artery occlusion (MCAO) and analyzed for infarct lesions.

Results: The images showed that the IP-treated mouse brains had significantly smaller infarct volumes compared to the control group. Of the MR sequences employed, the T2WI showed the highest level of correlations with postmortem infarct volume measurements.

Conclusions: The clinical 3T MR scanner turned out to have a solid potential as a practical tool for imaging small animal brains. MR sequences including DWI, T2WI, FLAIR were obtained with acceptable resolution and in a reasonable time constraint in evaluating a mouse stroke model brain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. L. Swain, R. L. Sabina, J. J. Hines, J. C. Greenfield Jr. and E. W. Holmes, Cardiovasc. Res. 18, 264 (1984).

    Article  Google Scholar 

  2. Y. M. Yang, X. Feng, Z. W. Yao, W. J. Tang, H. Q. Liu and L. Zhang, J. Neurosci. Methods 167, 176 (2008).

    Article  Google Scholar 

  3. A. Barrier et al., Dis. Colon Rectum 48, 2238 (2005).

    Article  Google Scholar 

  4. E. M. Park, T. H. Joh, B. T. Volpe, C. K. Chu, G. Song and S. Cho, Neuroscience 123, 147 (2004).

    Article  Google Scholar 

  5. P. G. Mullins, D. G. Reid, P. D. Hockings, S. J. Hadingham, C. A. Cambell, J. B. Chalk and D. M. Doddrell, MR Biomed. 14, 204 (2001).

    Google Scholar 

  6. T. N. Lin, Y. Y. He, G. Wu, M. Khan and C. Y. Hsu, Stroke 24, 117 (1993).

    Article  Google Scholar 

  7. K. Kitagawa et al., Brain Res. 528, 21 (1990).

    Article  Google Scholar 

  8. S. Cho, E. M. Park, P. Zhou, K. Frys, M. E. Ross and C. Iadecola, J. Cereb. Blood Flow Metab. 25, 493 (2005).

    Article  Google Scholar 

  9. A. Barrier et al., FASEB. J. 19, 1617 (2005).

    Article  Google Scholar 

  10. T. Ohtsuki, C. A. Ruetzler, K. Tasaki and J. M. Hallenbeck, J. Cereb. Blood Flow Metab. 16, 1137 (1996).

    Article  Google Scholar 

  11. F. C. Barone, R. F. White, P. A. Spera, P. A. Spera, J. Ellison, R. W. Currie, X. Wang and G. Z. Feuerstein, Stroke 29, 1937 (1998).

    Article  Google Scholar 

  12. J. A. Shin, E. M. Park, J. S. Choi, S. M. Seo, J. L. Kang, K. E. Lee and S. Cho, J. Neuroimmunol 217, 14 (2009).

    Article  Google Scholar 

  13. U. Dirnagl, R. P. Simon and J. M. Hallenbeck, Trends Neurosci. 26, 248 (2003).

    Article  Google Scholar 

  14. U. Dirnagl and A. Meisel, Neuropharmacology 55, 334 (2008).

    Article  Google Scholar 

  15. F. Chen, F. De Keyzer, H. Wang, V. Vandecaveye, W. Landuyt, H. Bosmans, R. Hermans, G. Marchal and Y. Ni, Methods 43, 12 (2007).

    Article  MATH  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Radiology, Ewha Womans University Hospital, Seoul, 158-710, Korea

    Soo Mee Lim

  2. Department of Pharmacology, Ewha Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea

    Eun Mi Park

  3. Department of Pharmacy, Ewha University College of Pharmacy, Seoul, 158-710, Korea

    In Kyoon Lyoo & Junghyun Lee

  4. Ewha Medical Research Institute, School of Medicine, Ewha Womans University, Seoul, 158-710, Korea

    Bo Mi Han

  5. Department of Radiology, Ewha Womans University Hospital, Seoul, 158-710, Korea

    Jeong Kyong Lee

  6. Department of Brain and Cognitive Sciences, Ewha Womans University, Seoul, 158-710, Korea

    Su Bin Lee

Authors
  1. Soo Mee Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eun Mi Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. In Kyoon Lyoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Junghyun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bo Mi Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jeong Kyong Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Su Bin Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Soo Mee Lim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lim, S.M., Park, E.M., Lyoo, I.K. et al. MR images of mouse brain using clinical 3T MR scanner and 4CH-Mouse coil. Journal of the Korean Physical Society 67, 237–242 (2015). https://doi.org/10.3938/jkps.67.237

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3938/jkps.67.237

Keywords

Search

Navigation