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Chromosome number and secondary chromosomal associations in wild populations of Geranium pratense L. from the cold deserts of Lahaul-Spiti (India)

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Abstract

In this work we studied the meiotic chromosome number and details of secondary chromosomal associations recorded for the first time in Geranium pratense L. from the alpine environments in the cold deserts of Lahaul-Spiti (India). All the presently studied individuals of the species existed at 4x level (x = 14). The present chromosome count of n = 28 in the species adds a new cytotype to the already existing diploid chromosome count of 2n = 28 from the Eastern Himalayas and outside of India. Out of the six accessions scored presently four showed normal meiotic course. However, two accessions investigated from Mud, 3800 m and Koksar, 3140 m depicted abnormal meiotic course due to the presence of multivalents and univalents, and secondary associations of bivalents/chromosomes. The secondary chromosomal associations in the species existed among bivalents/chromosomes were noticed in the PMCs at prophase-I (diakinesis) and persisted till the separation of sister chromatids at M-II. The variation in the number of bivalents/chromosomes involved in the secondary associations at M-I (2–8) and A-I/M-II (2–12) has also been recorded. The occurrence of such secondary associations of bivalents/chromosomes in G. pratense which existed at 4x level indicated the secondary polyploid nature of the species.

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References

  1. Darlington, C.D., Cytology, London: Churchill, 1965.

    Google Scholar 

  2. Kuwada, Y., A Cytological Study of Oryza sativa L., Bot. Mag., 1910, vol. 24, pp. 267–280.

    Google Scholar 

  3. Ishikawa, M., Cytologische Studien von Dahlien, Bot. Mag., 1911, vol. 25, pp. 1–8.

    Google Scholar 

  4. Marchal, E., Recherches Cytologiques sur le Genre Amblystegium, Bull. Soc. R. Bot. Belg., 1912, vol. 51, pp. 189–200.

    Google Scholar 

  5. Hagerup, O., Empetrum hermaphroditum (Lge.), a New Tetraploid, Bisexual Species, Dansk Botanisk Arkiv, 1927, vol. 5, no. 2.

    Google Scholar 

  6. Jacob, K.M., Secondary Associations in the Castor Oil Plant, Cytologia, 1957, vol. 22, pp. 380–392.

    Article  Google Scholar 

  7. Riley, R., The Secondary Pairing of Bivalents with Genetically Similar Chromosomes, Nature, 1960, vol. 185, pp. 751–752.

    Article  Google Scholar 

  8. Kempanna, C., Investigations into the Genetic Regulation of Meiotic Chromosome Behaviour in Triticum aestivum L., Ph.D. Thesis, University of Cambridge, 1963.

    Google Scholar 

  9. Kempanna, C. and Riley, R., Secondary Association between Genetically Equivalent Bivalents, Heredity, 1964, vol. 19, pp. 289–299.

    Article  Google Scholar 

  10. Gupta, P.P. and Roy, S.K., Primary and Secondary Chromosome Association in Euryale ferox Salisb, Cytologia, 1973, vol. 38, pp. 645–649.

    Google Scholar 

  11. Jelenkovic, G., Shifriss, Q., and Harington, E., Association and Distribution of Meiotic Chromosomes in a Haploid of Ricinus communis L., Cytologia, 1980, vol. 45, pp. 571–577.

    Article  Google Scholar 

  12. Agarwal, P.K., Secondary Association of Bivalents in Cissus discolor Blume, Cytologia, 1983, vol. 48, pp. 577–580.

    Article  Google Scholar 

  13. Malgwi, M.M., Oyewole, S.O., and Khan, A.U., Chromosomes and Secondary Associations in Tetraploid Cleome polyanthera L., Nucleus, 1997, vol. 40, pp. 20–25.

    Google Scholar 

  14. Argimyn, S., Wulff, A.F., and Xifreda, C.C., Chromosome Association and Basic Number in Anredera krapovickasii (Basellaceae), Caryologia, 1999, vol. 52, pp. 203–206.

    Article  Google Scholar 

  15. Sengupta, S. and Datta, A.K., Secondary Association of Bivalents in an Induced Mutant Line of Sesame (Sesamum indicum L.), J. Phytol. Res., 2002, vol. 15, pp. 13–16.

    Google Scholar 

  16. Sengupta, S. and Datta, A.K., Secondary Chromosome Associations in Control and Mutant Plant Types of Sesamum indicum L., Cytologia, 2003, vol. 68, pp. 141–145.

    Article  Google Scholar 

  17. Lopez, M.G., Wulf, A.F., Poggio, L., and Xifreda, C.C., Chromosome Numbers and Meiotic Studies in Species of Senecio (Asteraceae) from Argentina, Bot. J. Linn. Soc., 2005, vol. 148, pp. 465–474.

    Article  Google Scholar 

  18. Mukherjee, M. and Datta, A.K., Secondary Chromosome Associations in Ocimum basilicum L. and Ocimum tenuiflorum L., Cytologia, 2005, vol. 70, pp. 149–152.

    Article  Google Scholar 

  19. Mukherjee, M. and Datta, A.K., Secondary Chromosome Associations in Ocimum spp., Cytologia, 2006, vol. 71, pp. 149–152.

    Article  Google Scholar 

  20. Iqbal, M. and Datta, A.K., Cytogenetic Studies in Withania somnifera (L.) Dun. (Solanaceae), Cytologia, 2006, vol. 71, pp. 43–47.

    Google Scholar 

  21. Bhattacharya, A. and Datta, A.K., Secondary Chromosome Associations in Uraria picta (Jacq.) DC. (Family: Leguminosae), Cytologia, 2010, vol. 75, pp. 37–40.

    Article  Google Scholar 

  22. Sood, S.K., Nath, R., and Kalia, D.C., Ethnobotany of Cold Desert Tribes of Lahaul-Spiti (N. W. Himalaya), Delhi, India: Deep Publ., 2001.

    Google Scholar 

  23. Korner, Ch., Alpine Plant Diversity: A Global Survey and Functional Interpretations, in Arctic and Alpine Biodiversity: Patterns, Causes and Ecosystem Consequences, Chapin, F.S. and Korner, Ch., Eds., Berlin: Springer, 1995.

  24. Stöcklin, J., Kuss, P., and Pluess, A.R., Genetic Diversity, Phenotypic Variation and Local Adaptation in the Alpine Landscape: Case Studies with Alpine Plant Species, Bot. Helv., 2009, vol. 119, pp. 125–133.

    Article  Google Scholar 

  25. Aswal, B.S. and Mehrotra, B.N., Flora of Lahaul-Spiti (a Cold Desert in North West Himalaya), Dehra Dun, India, 1994.

    Google Scholar 

  26. Chowdhery, H.J. and Wadhwa, B.M., Flora of Himachal Pradesh: Analysis, Botanical Survey of India, Calcutta, 1984, vols. 1–3.

  27. Chauhan, N.S., Important Medicinal and Aromatic Plants of Himachal Pradesh, Indian For, 2003, vol. 129, pp. 979–998.

    Google Scholar 

  28. Sharma, P.K. and Lal, B., Ethnobotanical Notes on Some Medicinal and Aromatic Plants of Himachal Pradesh, Indian J. Tradit. Knowl., 2005, vol. 4, pp. 424–428.

    Google Scholar 

  29. Kala, C.P. and Prakash, C., Medicinal Plants of the High Altitude Cold Desert in India: Diversity, Distribution and Traditional Uses, Intl. J. Biodivers. Sci. Management, 2006, vol. 2, pp. 43–56.

    Article  Google Scholar 

  30. Singh, K.N. and Lal, B., Ethnomedicines Used Against Four Common Ailments by the Tribal Communities of Lahaul-Spiti in Western Himalaya, J. Ethnopharmacol., 2008, vol. 115, pp. 147–159.

    Article  PubMed  CAS  Google Scholar 

  31. Kumar, P. and Singhal, V.K., Cytology of Caltha palustris L. (Ranunculaceae) from Cold Regions of Western Himalayas, Cytologia, 2008, vol. 73, pp. 137–143.

    Article  Google Scholar 

  32. Kumar, P. and Singhal, V.K., Chromosome Number, Male Meiosis and Pollen Fertility in Selected Angiosperms of the Cold Deserts of Lahaul-Spiti and Adjoining Areas (Himachal Pradesh, India), Plant Syst. Evol., 2011, vol. 297, pp. 271–297.

    Article  Google Scholar 

  33. Kumar, P. and Singhal, V.K., Meiotic Aberrations and Chromosomal Variation in the Plants of Lahaul-Spiti and Adjoining High Hills in Himachal Pradesh, in Biodiversity Evaluation-Botanical Perspective, Atri, N.S., Gupta, R.C., Saggoo, M.I.S., and Singhal, V.K., Eds., Dehra Dun, India: Bishen Singh Mahendra Pal Singh, 2012, pp. 147–170.

    Google Scholar 

  34. Kumar, P. and Singhal, V.K., Male Meiosis, Morphometric Analysis and Distribution Pattern of 2× and 4× Cytotypes of Ranunculus hirtellus Royle, 1834 (Ranunculaceae) from the Cold Regions of Northwest Himalayas (India), Comp. Cytogenet., 2011, vol. 5, pp. 143–161.

    Article  Google Scholar 

  35. Kumar, P., Singhal, V.K., and Kaur, D., Meiotic Studies in Species from the Cold Deserts of Lahaul-Spiti and Adjoining Areas (Northwest Himalayas), Cytologia, 2008, vol. 73, pp. 463–470.

    Article  Google Scholar 

  36. Kumar, P., Singhal, V.K., Kaur, D., and Kaur, S., Cytomixis and Associated Meiotic Abnormalities Affecting Pollen Fertility in Clematis orientalis, Biol. Plant., 2010, vol. 54, pp. 181–184.

    Article  Google Scholar 

  37. Kumar, P., Singhal, V.K., and Kaur, D., Impaired Male Meiosis due to Irregular Synapsis Coupled with Cytomixis in a New Diploid Cytotype of Dianthus angulatus (Caryophyllaceae) from Indian Cold Deserts, Folia Geobot., 2012, vol. 47, pp. 59–68.

    Article  Google Scholar 

  38. Kumar, P., Singhal, V.K., Rana, P.K., Kaur, S., and Kaur, D., Cytology of Ranunculus laetus Wall. ex Royle from Cold Desert Regions and Adjoining Hills of North-west Himalayas, Caryologia, 2011, vol. 64, pp. 25–32.

    Google Scholar 

  39. Singhal, V.K. and Kumar, P., Impact of Cytomixis on Meiosis, Pollen Viability and Pollen Size in Wild Populations of Himalayan Poppy (Meconopsis aculeata Royle), J. Biosci., 2008, vol. 33, pp. 371–380.

    Article  PubMed  CAS  Google Scholar 

  40. Singhal, V.K. and Kumar, P., Variable Sized Pollen Grains Due to Impaired Male Meiosis in the Cold Desert Plants of Northwest Himalayas (India), in Pollen: Structure, Types and Effects, Kaiser, B.J. Ed., New York, USA: Nova Sci. Publ., 2010, pp. 101–126.

    Google Scholar 

  41. Gupta, R.C., Himshikha, Kumar, P., and Dhaliwal, R.S., Cytological Studies in Some Plants from Cold Deserts of India, Lahaul and Spiti (Himachal Pradesh), Chromosome Bot., 2009, vol. 4, pp. 5–11.

    Article  Google Scholar 

  42. Singhal, V.K., Kaur, D., and Kumar, P., Effect of Cytomixis on the Pollen Size in ‘Seabuckthorn’ (Hippophae rhamnoides L., Elaeagnaceae), Cytologia, 2008, vol. 73, pp. 167–172.

    Article  Google Scholar 

  43. Singhal, V.K., Kumar, P., Kaur, D., and Rana, P.K., Chromatin Transfer during Male Meiosis Resulted into Heterogeneous Sized Pollen Grains in Anemone rivularis Buch.-Ham. Ex DC. from Indian Cold Deserts, Cytologia, 2009, vol. 74, pp. 229–234.

    Article  Google Scholar 

  44. Singhal, V.K., Rana, P.K., Kumar, P., and Kaur, D., Persistent Occurrence of Meiotic Abnormalities in a New Hexaploid Cytotype of Thalictrum foetidum L. from Indian Cold Deserts, Biologia, 2011, vol. 66, pp. 458–464.

    Article  Google Scholar 

  45. Kumar, P., Exploration of Cytomorphological Diversity in the Members of Polypetalae from Lahaul-Spiti and Adjoining Areas, Ph.D. Thesis, Patiala, Punjab, India: Punjabi Univ., 2011.

    Google Scholar 

  46. Chatterjee, A. and Sharma, A.K., Chromosome Study in Geraniales, Nucleus, 1970, vol. 13, pp. 179–200.

    Google Scholar 

  47. Sharma, A.K., Chromosome Number Reports of Plants. Annual Report. Cytogenetics Laboratory of Department of Botany, University Calcutta, Res. Bull., 1970, vol. 2, pp. 1–50.

    Google Scholar 

  48. Gauger, W., Ergebnisse Einer Zytologischen Untersuchung der Familie der Geraniaceae, Planta, 1937, vol. 26, pp. 529–531.

    Article  Google Scholar 

  49. Warburg, E.F., Taxonomy and Relationships in the Geraniales in the Light of Their Cytology, New Phytol., 1938, vol. 37, pp. 189–210.

    Article  Google Scholar 

  50. Löve Á. and Löve, D., Cytotaxonomical Conspectus of the Iceland Flora, Acta Horti. Gotob., 1956, vol. 20, pp. 65–290.

    Google Scholar 

  51. Zhukova, P.G., Karyology of Some Plants, Cultivated in the Arctic-Alpine Botanical Garden, in Plantarum in Zonam Polarem Transportation, Avrorin, N.A., Ed., Leningrad, 1967, vol. 2, pp. 139–149.

    Google Scholar 

  52. Majovsky, J., et al., Index of Chromosome Numbers of Slovakian Flora (Part 1), Acta Fac. Rer. Nat. Univ. Comenianae Bot., 1970, vol. 16, pp. 1–26.

    Google Scholar 

  53. Wahlstrom, R., Geranium pretense-a Species New to Northern Norway, Blyttia, 1978, vol. 36, pp. 91–94.

    Google Scholar 

  54. Skalinska, M., Pogan, E., Czapik, R., et al., Further Studies in Chromosome Numbers of Polish Angiosperms, XII, Acta Biol. Cracov. Ser. Bot., 1978, vol. 21, pp. 31–63.

    Google Scholar 

  55. Javurkova-Jarolimova, V., In IOPB Chromosome Number Reports LXIV, Taxon, 1979, vol. 28, pp. 400–401.

    Google Scholar 

  56. van Loon, J.Chr., Hybridization Experiments in Geranium, Genetics, 1984, vol. 65, pp. 167–171.

    Google Scholar 

  57. Parfenov, V.I. and Dmitrieva, S.A., Karyological Characteristics of Representatives of Vascular Plant Flora of the Berezina Biosphere Reserve, Zapov. Belorus. Issl., 1988, vol. 12, pp. 3–8.

    Google Scholar 

  58. Kiehn, M., Vitek, E., Hellmayr, E., Walter, J., Tschenett, J., Justin, C., and Mann, M., Beitrage zur Flora von Osterreich: Hromosomenzahlungen, Verh. Zool. Bot. Ges. Wien, 1991, vol. 128, pp. 19–39.

    Google Scholar 

  59. Krasnikov, A.A., Chromosome Numbers in Some Species of Vascular Plants from Novosibirsk Region, Bot. Zh., 1991, vol. 76, pp. 476–479.

    Google Scholar 

  60. Hollingsworth, P.M., Gornall, R.J., and Bailey, J.P., Contribution to a Cytological Catalogue of the British and Irish Flora, 2, Watsonia, 1992, vol. 19, pp. 134–137.

    Google Scholar 

  61. Dobes, C.H., Hahn, B., and Morawetz, W., Chromosomenzahlen zur Gefasspflanzen-Flora Osterreichs, Linzer Biol. Beitrage, 1997, vol. 29, pp. 5–43.

    Google Scholar 

  62. Albers F. and Probsting, W., In Standardliste der Farn- und Blutenpflanzen Deutschlands, Wisskirchen, R. and Haeupler, H., Eds., Bundesamt fur Naturschutz and Verlag Eugen Ulmer, Stuttgart, 1998.

  63. Petrova, A. and Stanimirova, P., Mediterranean Chromosome Number Reports 11 (1248–1253), Fl. Medit., 2001, vol. 11, pp. 460–466.

    Google Scholar 

  64. Tischler, G., Die Bedeutung der Polyploidie fur die Verbreitung der Angiospermen, erlautert an den Arten Schleswig-Holsteins, mit Ausblicken auf andere Florengebiete, Bot. Jahrb. Syst., 1934, vol. 67, pp. 1–36.

    Google Scholar 

  65. Tjebbes, K., The Chromosome Numbers of Some Flowering Plants, Hereditas, 1928, vol. 10, pp. 328–332.

    Article  Google Scholar 

  66. Belyaeva, V.A. and Siplivinsky, V.N., Chromosome Numbers and Taxonomy of Some Species of Baikal Flora, Bot. Zh., 1975, vol. 60, pp. 864–887.

    Google Scholar 

  67. Darlington, C.D., Studies in Prunus. I and II, J. Genet., 1928, vol. 19, pp. 213–256.

    Article  Google Scholar 

  68. Lawrence, J.C., The Secondary Association of Chromosomes, Cytologia, 1931, vol. 2, pp. 352–384.

    Article  Google Scholar 

  69. Brown, W.S., Spurious Secondary Association and Asymmetric Spindles in Luzula, Cytologia, 1950, vol. 15, pp. 259–268.

    Article  Google Scholar 

  70. Stebbins, G.L., Variation and Evolution in Plants, New York: Columbia Univ., 1950.

    Google Scholar 

  71. Olson, M., Bayesian Procedures for Discriminating among Hypotheses with Discrete Distributions: Inheritance in the Tetraploid Astilbe Biternub, Genetics, 1997, vol. 147, pp. 1933–1942.

    PubMed  CAS  Google Scholar 

  72. Lichter, P., Multilocular FISHing: What’s the Catch? Trend Genet., 1997, vol. 13, pp. 475–478.

    Article  CAS  Google Scholar 

  73. Bhojwani, S.S. and Bhatnagar, S.P., The Embryology of Angiosperm, New Delhi, India: Vikas, 1999.

    Google Scholar 

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  1. Department of Botany, Punjabi University, Patiala, 147 002, Punjab, India

    P. Kumar & V. K. Singhal

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Kumar, P., Singhal, V.K. Chromosome number and secondary chromosomal associations in wild populations of Geranium pratense L. from the cold deserts of Lahaul-Spiti (India). Cytol. Genet. 47, 107–114 (2013). https://doi.org/10.3103/S0095452713020084

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