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Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle)

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Abstract

We report the occurrence of cytomixis in wild populations of Himalayan poppy (Meconopsis aculeata Royle), which is considered to be an important and threatened medicinal plant growing in the high hills of the Himalayas. The impact of cytomixis on meiotic behaviour, reduced pollen viability and heterogeneous-sized pollen grains was also studied. Cytological studies in the seven wild populations from the high hills of Himachal Pradesh revealed that all the Himalayan populations exist uniformly at the tetraploid level (2n=56) on x=14. The phenomenon of chromatin transfer among the proximate pollen mother cells (PMCs) in six populations caused various meiotic abnormalities. Chromatin transfer also resulted in the formation of coenocytes, aneuploid, polyploid and anucleated PMCs. Among individuals that showed chromatin transfer, chromosome stickiness and interbivalent connections were frequently observed in some PMCs. The phenomenon of cytomixis in the species seems to be directly under genetic control; it affects the meiotic course considerably and results in reduced pollen viability.

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References

  • Ajay K J and Sarbhoy R K 1987 Cytogenetical studies on the effect of some chlorinated pesticides II. Effect on meiotic chromosomes of Lens and Pisum; Cytologia 52 55–61

    Article  Google Scholar 

  • Akpabio K E 1990 Chromosomal interconnections and metaphase 1 clumping in meiosis of four species of Crotalaria L.; Nig.J. Bot. 3 191–195

    Google Scholar 

  • Alice M S and Maria S P 1997 Cytomixis in Brassica napus var. oleifera and Brassica campestris var. oleifera (Brassicaceae); Cytologia 62 25–29

    Article  Google Scholar 

  • Ashraf M and Gohil R N 1994 Cytology of legumes of Kashmir Himalaya V. Cytomixis and chromosome migration in Astragalus subuliformis DC; Nucleus 37 119–122

    Google Scholar 

  • Bahl J R and Tyagi B R 1988 Cytomixis in pollen mother cells of Papaver dubium L.; Cytologia 53 771–775

    Article  Google Scholar 

  • Bedi Y S 1990 Cytomixis in woody species; Proc. Indian Natl. Sci. Acad. (Plant Sci.) 100 B 233–238

    Google Scholar 

  • Bell C R 1964 Cytomixis in Tauschia nudicaulis Schlecht (Apiaceae); Cytologia 29 369–398

    Article  Google Scholar 

  • Bellucci M, Roscini C and Mariani A 2003 Cytomixis in pollen mother cells of Medicago sativa L.; J. Heredity 94 512–516

    Article  CAS  Google Scholar 

  • Bhat T A, Parveen S and Khan A H 2006 MMS-induced cytomixis in pollen mother cells of broad bean (Vicia faba L.); Turk. J. Bot. 30 273–279

    Google Scholar 

  • Bione N C P, Pagliarini M S and de Toledo J F F 2000 Meiotic behavior of several Brazilian soybean varieties; Genet. Mol. Biol. 23 623–631

    Article  Google Scholar 

  • Bobak M and Herich R 1978 Cytomixis as a manifestation of pathological changes after the application of trifuraline; Nucleus 21 22–26

    Google Scholar 

  • Cooper D D 1952 The transfer of deoxyribose nucleic acid from the tapetum to the microsporocytes at the onset of meiosis; Cytologia 86 271–274

    Google Scholar 

  • Consolaro M E L and Pagliarini M S 1995 Cytomixis in pollen mother cells of Centella asiatica L.; Nucleus 38 80–85

    Google Scholar 

  • Chauhan A K S 1981 Cytomixis in Papaver rhoeas; Perspectives in cytology and genetics; in Proceedings of the third All India Congress of Cytology and Genetics (eds) G K Manna and U Sinha (New Delhi: Hindasia Publishers) pp 309–312

    Google Scholar 

  • Dagne K 1994 Meiosis in interspecific hybrids and genomic interrelationships in Guizotia Cass. (Compositae); Hereditas 121 119–129

    Article  Google Scholar 

  • Datta A K, Mukherjee M and Iqbal M 2005 Persistent cytomixis in Occimum basilicum L. (Lamiaceae) and Withania somnifera (L.) Dun (Solanaceae); Cytologia 70 309–313

    Article  Google Scholar 

  • De M and Sharma A K 1983 Cytomixis in pollen mother cells of an apomictic ornamental Ervatamia divaricata (Linn.) Alston; Cytologia 48 201–207

    Article  Google Scholar 

  • de Nettancourt D and Grant W F 1964 Lacytogénétiquede Lotus (Leguminosae) III. Un cas de cytomixie dans un hybride interspécifique; Cytologia 29 191–195

    Article  Google Scholar 

  • de Souza A and Pagliarini M 1997 Cytomixis in Brassica napus var. oleifera and Brassica campestris var. oleifera (Brassicaceae); Cytologia 62 25–29

    Article  Google Scholar 

  • Dhar U, Manjkhola S, Joshi M, Bhatt A, Bisht A K and Meena Joshi 2002 Current status and future strategy for development of medicinal plants sector in Uttaranchal, India; Curr. Sci. 86 956–964

    Google Scholar 

  • Ernst W R 1965 Meconopsis in documented chromosome numbers of plants; Madrono 18 123–126

    Google Scholar 

  • Falistocco E, Tosti N and Falcinelli M 1995 Cytomixis in pollen mother cells of diploid Dactylis one of the origins of 2n gametes; J. Heredity 86 448–453

    Article  Google Scholar 

  • Falusi O A 2006 Interchromosomal connections and metaphase 1 clumping in meiosis of two Capsicum Linn. species in Nigeria; Afr. J. Biotechnol. 5 2066–2068

    CAS  Google Scholar 

  • Ghaffari G M 2006 Occurrence of diploid and polyploid microspores in Sorghum bicolor (Poaceae) is the result of cytomixis; Afr. J. Biotechnol. 5 1450–1453

    Google Scholar 

  • Ghanima A M and Talaat A A 2003 Cytomixis and its possible evolutionary role in a Kuwait population of Diplotaxis harra (Boraginaceae); Bot. J. Linn. Soc. 143 169–175

    Article  Google Scholar 

  • Gottschalk W 1970 Chromosome and nucleus migration during microsporogenesis of Pisum satium; Nucleus 13 1–9

    Google Scholar 

  • Guzicka M and Wozny A 2005 Cytomixis in shoot apex of Norway spruce [Picea abies (L.) Karst.]; Trees 18 722–724

    Article  Google Scholar 

  • Haroun S A 1995 Cytomixis in pollen mother cells of Polygonum tomentosum Schrank; Cytologia 60 257–260

    Article  Google Scholar 

  • Haroun S A 1996 Induced cytomixis and male sterility in pollen mother cells of Hordeum vulgare L.; Delta J. Sci. 20 172–183

    Google Scholar 

  • Haroun S A, Al Shehri A M and Al Wadie H M 2004 Cytomixis in the microsporogenesis of Vicia faba L. (Fabaceae); Cytologia 69 7–11

    Article  Google Scholar 

  • Heslop-Harrison J 1966 Cytoplasmic connexions between angiosperm meiocytes; Ann. Bot. 30 221–234

    Article  Google Scholar 

  • Holmgren P K and Keuken W 1974 Index herbariorum Part I 6th edition Reg. Veg. 92VII pp 397

    Google Scholar 

  • Hooker J D 1872 The flora of British India I (Ashford, Kent, England: L Reeve and Co.)

    Google Scholar 

  • Jacob K T 1941 Certain abnormalities in the root tips of cotton; Curr. Sci. 10 174–175

    Google Scholar 

  • Jee V, Dhar U and Kachroo 1989 Cytogeography of some endemic taxa of Kashmir Himalaya; Proc. Indian Natl. Sci. Acad. (Bio. Sci.) B55 177–184

    Google Scholar 

  • Kagawa F 1929 Cytological studies on the pollen formation of the hybrids between Triticum and Aegilops; Jpn. J. Bot. 4 345–361

    Google Scholar 

  • Kala C P 2000 Status and conservation of rare and endangered medicinal plants in the Indian trans-Himalaya; Biol. Cons. 93 371–379

    Article  Google Scholar 

  • Kihara H and Lilienfeld F 1934 Kerneiwanderung und bildung syndipolider Mutterzeren bei dem F1 Bastard Triticum aegilopoides x Aegilops squarosa; Jpn. J. Genet. 10 1–28

    Article  Google Scholar 

  • Kornicke M 1901 Uber ortsveranderung von Zellkarnern S B Niederhein Ges Natur-U Heilkunde Bonn A pp 14–25

  • Koul K K 1990 Cytomixis in pollen mother cells of Alopecurus arundinaceus Poir.; Cytologia 55 169–173

    Article  Google Scholar 

  • Lattoo S K, Khan S, Bamotra S and Dhar A K 2006 Cytomixis impairs meiosis and influences reproductive success in Chlorophytum comosum (Thunb) Jacq. — an additional strategy and possible implications; J. Biosci. 31 629–637

    Article  CAS  Google Scholar 

  • Mary T N 1979 Cytomixis in Althea rosea L.; Indian J. Bot. 2 80–82

    Google Scholar 

  • Mary T N and Suvarnalatha B 1981 Cytomixis and deviation of chromosome numbers in pollen mother cells of Gossypium species; J. Indian Bot. Soc. 60 74

    Google Scholar 

  • Mehra P N and Kalia V 1973 Accessory chromosomes and multinucleate pollen mother cells in Saccharum benghalense Retz. Complex; Nucleus 16 75–78

    Google Scholar 

  • Mendes-Bonato A B, Pagliarini M S, Silva N and Valle C B 2001 Meiotic instability in invader plants of signal grass Brachiaria decumbens Stapf (Gramineae); Genet. Mol. Biol. 23 619–625

    Google Scholar 

  • Mendes E J and Rijo L 1951 A new interpretation for cytomixis; Portug. Acta. Biol. A-3 211–288

    Google Scholar 

  • Morikawa T and Leggett M 1996 Cytological and morphological variations in wild populations of Avena canariensis from the Canary Islands; Genes Genet. Syst. 71 15–21

    Article  Google Scholar 

  • Morrisset P 1978 Cytomixis in pollen mother cells of Ononis (Leguminosae); Can. J. Genet. Cytol. 20 383

    Article  Google Scholar 

  • Nakajima G 1954a Genetical and cytological studies in the breeding of amphidiploid type between Triticum and Secale; Jpn. J. Breeding 3 47–52

    Article  Google Scholar 

  • Nakajima G 1954b Meiosis of pollen mother cells of T. compactum × S. cereale F2 plants with 2n=56 and 58 chromosomes; Jpn. J. Breeding 3 53–58

    Article  Google Scholar 

  • Narain P 1976 Cytomixis in pollen mother cells of Hemerocallis Linn.; Curr. Sci. 48 996–998

    Google Scholar 

  • Nirmala A and Rao P N 1996 Genesis of chromosome numerical mosaicism in higher plants; Nucleus 39 15–175

    Google Scholar 

  • Omara M K 1976 Cytomixis in Lolium perenne; Chromosoma 55 267–271

    Article  Google Scholar 

  • Pierozzi N I and Benatti R 1998 Cytological analysis in the microsporogenesis of ramie Boehmeria nivea Gaud. (Urticaceae) and the effect of colchicine on the chiasma frequency; Cytologia 63 213–221

    Article  CAS  Google Scholar 

  • Poggio L, Rosoto M, Mazoti L B and Naranjo C A 1997 Variable meiotic behaviour among plants of an alloplasmic line of maize; Cytologia 62 271–274

    Article  Google Scholar 

  • Price S 1956 Cytological studies in Saccharum and allied genera 1. Syncytes in certain clones of Saccharum and Erianthus; Cytologia 21 21–37

    Article  Google Scholar 

  • Ratter J A 1968 Cytological studies in Meconopsis; Notes R. Bot. Gard. Edinburgh 28 191–200

    Google Scholar 

  • Sapre A B and Deshpande D S 1987 A change in chromosome number due to cytomixis in an interspecific hybrid of Coix L.; Cytologia 52 167–174

    Article  Google Scholar 

  • Sarvella P 1958 Cytomixis and loss of chromosomes in meiotic and somatic cells of Gossypium; Cytologia 23 14–24

    Article  Google Scholar 

  • Semyarkhina S Y A and Kuptsou M S 1974 Cytomixis in various forms of sugarbeet; Vests I ANBSSE Ser. Biyal. 4 43–47

    Google Scholar 

  • Sharma C M and Gaur R D 1987 Palynotaxonomy of Himalayan Blue poppy (Meconopsis aculeata Royle); Curr. Sci. 56 551

    Google Scholar 

  • Singhal V K and Gill B 1985 Cytomixis in some woody species; Biologica 1 168–175

    Google Scholar 

  • Singhal V K, Gill B S and Dhaliwal R S 2007 Status of chromosomal diversity in the hardwood tree species of Punjab state; J. Cytol. Genet. 8 67–83

    Google Scholar 

  • Sugiura T 1937 A list of chromosome numbers in angiospermous plants III; Bot. Mag. 51 425–426

    Article  Google Scholar 

  • Sun G, Yen C and Yang J 1993 Intermeiocyte connections and cytomixis in intergeneric hybrids II Triticum aestivum × Psathyrostachys huashanica; Wheat Inf. Serv. 77 13–18

    Google Scholar 

  • Sun G, Yen C and Yang J 1994 Intermeiocyte connections and cytomixis in intergeneric hybrids III Roegneria tsukushiensis × Psathyrostachys huashanica; Wheat Inf. Serv. 79 24–27

    Google Scholar 

  • Tarkowska J 1960 Cytomixis in the epidermis of scales and leaves and in meristems of root apex of Allium cepa L.; Acta Soc. Bot. Pol. 29 149–168

    Article  Google Scholar 

  • Tarkowska J 1965 Experimental analysis of the mechanism of cytomixis I. Cytomixis in vegetative tissues; Acta Soc. Bot. Pol. 34 27–44

    Article  Google Scholar 

  • Tsarong and Tsewang J 1994 Tibetan medicinal plants (India: Tibetan Medical Publications). ISBN No. 81-900489-0-2

    Google Scholar 

  • Villax E and Mota M 1953 Behavior of a Triticum × Secale hybrid under the action of colchicines; Nature (London) 172 412–413

    Article  CAS  Google Scholar 

  • Wang R R C 1988 Coenocytism, ameiosis, and chromosome diminution in intergeneric hybrids in the perennial Triticeae; Genome 30 766–775

    Article  Google Scholar 

  • Yen C, Yan J L and Sun G L 1993 Intermeiocyte connections and cytomixis in intergeneric hybrid of Roegneria ciliaris (Trin.) Nevski with Psathyrostachys huashanica Keng; Cytologia 58 187–193

    Article  Google Scholar 

  • Ying M, Xie H-Y, Nie Z-L, Gul Z-J and Yang Y-P 2006 A karyomorphological study on four species of Meconopsis Vig. (Papaveraceae) from the Hengduan Mountains SW China; Caryologia 59 1–6

    Article  Google Scholar 

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  1. Department of Botany, Punjabi University, Patiala, 147 002, India

    V. K. Singhal & Puneet Kumar

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  1. V. K. Singhal
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Singhal, V.K., Kumar, P. Impact of cytomixis on meiosis, pollen viability and pollen size in wild populations of Himalayan poppy (Meconopsis aculeata Royle). J Biosci 33, 371–380 (2008). https://doi.org/10.1007/s12038-008-0057-0

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