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In vitro studies to produce double haploid in Indica hybrid rice

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Abstract

The aim of this investigation was to improve in vitro the technique of production of double haploid in Indica hybrid rice by combining anther culture, hormone shock and doubling chromosome. It was discussed how to avoid somaclonal variation during culturing and to reduce the time of this process. The anthers of KDML 105 × SPR 1 (Indica × Indica) were cultured in Linsmaier and Skoog (LS) medium, which contained nutrients, growth regulators [(2,4,-dichlorophenoxy acetic acid (2,4-D) and naphthalene acetic acid (NAA)] and organic compounds, and then subcultured by inducing embryo-like structure (ELS) LS media. During 4 weeks used LS media supplemented with 10 μM KNO3 + 2 mg/L 2,4-D + 2 mg/L NAA + 20% coconut water + 1 mg/L of activated charcoal had induced high embryogenic frequent callus with length of 4–5 mm. The supplementation of 0.2 g/L colchicine and 100 μM 2,4-D was the most efficient in LS media. Over 70% of viable double haploid ELS were produced in 8 weeks and subcultured only twice compared with conventional anther which takes more than 12 weeks. This new technique can therefore be applied to rice in order in shorten time to produce higher number of double haploid plantlets.

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Abbreviations

2,4-D, 2,4:

dichlorophenoxy acetic acid

ELS:

embryo-like structure

KDML:

Thai rice variety of donor plants

LS:

Linsmaier and Skoog

NAA:

naphthalene acetic acid

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Authors and Affiliations

  1. Department of Plant Science and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand

    Piyachai Premvaranon, Suchada Vearasilp, Sa-nguansak Thanapornpoonpong & Dumnern Karladee

  2. Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, 10900, Thailand

    Piyachai Premvaranon

  3. Postharvest Technology Research Institute, Chiang Mai University, Chiang Mai, 50200, Thailand

    Suchada Vearasilp & Sa-nguansak Thanapornpoonpong

  4. Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok, 10400, Thailand

    Suchada Vearasilp & Sa-nguansak Thanapornpoonpong

  5. Institute for Drug Research, School of Pharmacy, The Hebrew University — Hadassah Medical School, Jerusalem, Israel

    Shela Gorinstein

Authors
  1. Piyachai Premvaranon
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  2. Suchada Vearasilp
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  3. Sa-nguansak Thanapornpoonpong
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  4. Dumnern Karladee
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  5. Shela Gorinstein
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Premvaranon, P., Vearasilp, S., Thanapornpoonpong, Sn. et al. In vitro studies to produce double haploid in Indica hybrid rice. Biologia 66, 1074–1081 (2011). https://doi.org/10.2478/s11756-011-0129-8

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  • DOI: https://doi.org/10.2478/s11756-011-0129-8

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