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Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

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Abstract

The interaction between double-strand calf thymus gland DNA (ds-DNA) and Al(III) was studied by using differential pulse voltammetry (DPV) at a hanging mercury drop electrode (HMDE), Raman spectrometry and circular dichroism (CD) spectra. It was shown that at neutral pH ds-DNA did not produce any cathodic peak at the HMDE in the potential window from −550 to −2000 mV vs. SCE. However, in the presence of Al(III), a cathodic peak was generated at about −1660 mV, which is ascribed to a reduction of adenine and cytosine residues of single denatured DNA (sd-DNA). It was concluded that ds-DNA was completely denatured to sd-DNA by Al(III) at a neutral pH. The apparent denaturing kinetic velocity constants of ds-DNA by Al(III) were derived from linear increases of the cathodic peak currents with time. When [Al(III)]×[OH−]3 ≥ 2 × 10−26, the precipitation of Al(OH)3 was observed and identified by the Raman spectrum, and inductively coupled plasma atomic emission spectrometry (ICP-AES). CD spectra showed that the B-type of structure conformations of ds-DNA and related sd-DNA did not change with the increment of Al(III) from 5.0 × 10−7 to 1.0 × 10−5 M, but the corresponding absorption strengths increased. The related physiological significances and possible applications of the observations were considered.

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References

  1. M. Gomez, J. L. Esparza, M. Cabre, T. Garcia, and J. L. Domingo, Toxicology, 2008, 249, 214.

    Article  CAS  PubMed  Google Scholar 

  2. M. Kawahara, K. Konoha, T. Nagata, and Y. Sadakane, Biomedical Res. Trace Elements, 2007, 18, 211.

    CAS  Google Scholar 

  3. I. Shcherbatykh and D. O. Carpenter, J. Alzheimer’s Dis., 2007, 11, 191.

    Article  CAS  Google Scholar 

  4. R. A. Yokel, J. Alzheimer’s Dis., 2006, 10, 223.

    Article  Google Scholar 

  5. S. Mario, B. Silvia, and Z. Paolo, J. Alzheimer’s Dis., 2009, 17, 81.

    Article  Google Scholar 

  6. M. Meyer-Baron, M. Schäper, G. Knapp, and C. Van Thriel, Neurotoxcology, 2007, 28, 1068.

    Article  CAS  Google Scholar 

  7. B. Hartzell and B. McCord, Electrophoresis, 2005, 26, 1046.

    Article  CAS  PubMed  Google Scholar 

  8. J. Anastassopoulou, J Mol. Struct., 2003, 651, 19.

    Article  Google Scholar 

  9. J. Sponer, M. Sabat, J. V. Burda, J. Leszczynski, H. Jerzy, P. Hobza, and B. Lippert, J. Biolog. Inorg. Chem., 1999, 4, 537.

    Article  CAS  Google Scholar 

  10. J. V. Burda, J. Sponer, J. Leszczynski, H. Jerzy, and P. Hobza, J. Phys. Chem., 1997, 101, 9670.

    Article  CAS  Google Scholar 

  11. F. E. Rossetto and E. Nieboer, J. Inorg. Biochem., 1994, 54, 167.

    Article  CAS  PubMed  Google Scholar 

  12. G. L. Eichhorn and Y. A. Shin, J. Am. Chem. Soc., 1968, 90, 7323.

    Article  CAS  PubMed  Google Scholar 

  13. H. Zhou, X. Wu, and J. Yang, Talanta, 2009, 78, 809.

    Article  CAS  PubMed  Google Scholar 

  14. N. Graf, M. Goeritz, K. Mareike, and R. Kraemer, Angew. Chem. Int. Ed., 2006, 45, 4013.

    Article  CAS  Google Scholar 

  15. G. D. Liu, X. Yang, Z. P. Chen, G. L. Shen, and R. Q. Yu, Anal. Sci., 2000, 16, 1255.

    Article  CAS  Google Scholar 

  16. X. Jia and L. G. Marzilli, Biopolymers, 1991, 31, 23.

    Article  CAS  PubMed  Google Scholar 

  17. P. D. L. Lima, D. S. Leite, M. C. Vasconcellos, B. C. Cavalcanti, R. A. Santos, L. V. Costa-Lotufo, C. Pessoa, M. O. Moraes, and R. R. Burbano, Food Chem. Toxicol., 2007, 45, 1154.

    Article  CAS  PubMed  Google Scholar 

  18. P. D. L. Lima, D. S. Leite, M. C. Vasconcelles, B. C. Cavalcanti, R. A. Santos, L. V. Costa-Lotufo, C. Pessoa, M. O. Moraes, and R. R. Burbano, Food Chem. Toxicol., 2007, 45, 1154.

    Article  CAS  PubMed  Google Scholar 

  19. A. Lankoff, A. Banasik, A. Duma, E. Ochniak, H. Lisowska, T. Kuszewski, S. Góz´dz´, and A. Wojcik, Toxicol. Lett., 2006, 161, 27.

    Article  CAS  PubMed  Google Scholar 

  20. J. M. Howard, J. Nutr. Environ. Med., 2002, 12, 19.

    Article  CAS  Google Scholar 

  21. S. J. Karlik, G. L. Eichhorn, P. N. Lewis, and D. R. Crapper, Biochemistry, 1980, 19, 5991.

    Article  CAS  PubMed  Google Scholar 

  22. K. S. Latha, S. Anitha, K. S. J. Rao, and M. A. Viswamitra, Biochim. Biophys. Acta, 2002, 1588, 56.

    Article  CAS  PubMed  Google Scholar 

  23. J. Wu, F. Du, P. Zhang, I. A. Khan, J. Chen, and Y. Liang, J. Inorg. Biochem., 2005, 99, 1145.

    Article  CAS  PubMed  Google Scholar 

  24. K. S. J. Rao and S. Divark, Bull. Environ. Contam. Toxicol., 1993, 50, 92.

    CAS  PubMed  Google Scholar 

  25. Z. S. Yang, Y. L. Wang, Y. C. Liu, and G. C. Zhao, Anal. Sci., 2005, 21, 1355.

    Article  CAS  PubMed  Google Scholar 

  26. Z. S. Yang, Y. L. Wang, and G. C. Zhao, Anal. Sci., 2004, 20, 1127.

    Article  CAS  PubMed  Google Scholar 

  27. P. Palecˇek and B. D. Frary, Arch. Biochem. Biophys., 1966, 115, 431.

    Article  PubMed  Google Scholar 

  28. D. L. Smith and P. J. Elving, J. Am. Chem. Soc., 1962, 84, 1412.

    Article  CAS  Google Scholar 

  29. P. J. E. Palecˇek, M. Tomschik, V. Stañková, and L. Havran, Electroanalysis, 1997, 9, 990.

    Article  Google Scholar 

  30. P. Valenta, H. W. Nürnberg, and P. Klahre, Bioelectrochem. Bioenerg., 1974, 1, 487.

    Article  CAS  Google Scholar 

  31. B. Janik and P. J. Elving, Chem. Rev., 1968, 68, 295.

    Article  CAS  PubMed  Google Scholar 

  32. L. Trnkovaˇ, M. Stunicˇkovaˇ, and E. Palecˇek, J. Electroanal. Chem., 1980, 116, 643.

    Article  Google Scholar 

  33. M. Stunicˇkovaˇ, L. Trnkovaˇ, and J. Zetek, J. Electroanal. Chem., 1989, 275, 83.

    Article  Google Scholar 

  34. M. Fojta, R. P. Bowater, V. Stañková, L. Havran, D. M. J. Lilley, and E. Palecˇek, Biochemistry, 1998, 37, 4853.

    Article  CAS  PubMed  Google Scholar 

  35. K. S. J. Rao, B. S. Rao, D. Vishnuvardhan, and K. V. S. Prasad, Biochim. Biophys. Acta, Gene Struct. Expression, 1993, 1172, 17.

    Article  CAS  Google Scholar 

  36. M. Yamada, M. Yokoda, M. Kaya, S. Satoh, B. Jonganurakkun, M. Nomizu, and N. Nishi, Polymer, 2005, 46, 10102.

    Article  CAS  Google Scholar 

  37. M. T. Rajan, D. Champion, C. S. Kumar, D. Vishnuvardhan, K. S. J. Rao, and M. A. Viswamitra, Mol. Biol. Rep., 1996, 22, 47.

    Article  CAS  Google Scholar 

  38. D. Dyrssen, C. Haraldsson, E. Nyberg, and M. Wedborg, J. Inorg. Biochem., 1987, 29, 67.

    Article  CAS  PubMed  Google Scholar 

  39. R. Y. Zhang, Y. Liu, D. W. Pang, R. X. Cai, and Y. P. Qi, Anal. Sci., 2002, 18, 761.

    Article  CAS  PubMed  Google Scholar 

  40. F. P. Zhang, M. Zhang, J. J. Chen, L. Yang, M. Ji, and S. P. Bi, Anal. Sci., 2007, 23, 1325.

    Article  CAS  PubMed  Google Scholar 

  41. J. Liu, Z. H. Wang, G. A. Luo, Q. W. Li, and H. W. Sun, Anal. Sci., 2002, 18, 751.

    Article  CAS  PubMed  Google Scholar 

  42. J. B. Lee, M. Barch, E. W. Carstner, J. J. Völker, and K. J. Breslauer, Biochemistry, 2007, 46, 10756.

    Article  CAS  PubMed  Google Scholar 

  43. T. Nishimura, T. Okobra, A. M. Kelly, N. Shimada, Y. Takeda, and K. Sakurai, Biochemistry, 2007, 46, 8151.

    Article  Google Scholar 

  44. G. D. Fasman, Coord. Chem. Rev., 1996, 149, 125.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Chemistry & Chemical Engineering, State Key Laboratory of Coordination Chemistry of China & MOE Key Laboratory for Life Science, Nanjing University, 210093, Nanjing, China

    Fuping Zhang, Qing Cao, Jiongjia Cheng, Caihua Zhang, Na An & Shuping Bi

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  1. Fuping Zhang
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  2. Qing Cao
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  3. Jiongjia Cheng
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  4. Caihua Zhang
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  5. Na An
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  6. Shuping Bi
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Correspondence to Shuping Bi.

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Zhang, F., Cao, Q., Cheng, J. et al. Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH. ANAL. SCI. 25, 1019–1023 (2009). https://doi.org/10.2116/analsci.25.1019

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  • DOI: https://doi.org/10.2116/analsci.25.1019

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