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Square-wave Adsorptive Anodic Stripping Voltammetric Determination of Antidiabetic Drug Linagliptin in Pharmaceutical Formulations and Biological Fluids Using a Pencil Graphite Electrode

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Abstract

A simple, sensitive, low-cost, quick and reliable square-wave anodic stripping voltammetric method is described for the determination of the antidiabetic drug Linagliptin (LNG) in pure form, tablets, and spiked human urine and plasma samples. Using a pencil graphite electrode (PGE), cyclic voltammetry (CV) was applied to study the electrochemical behavior of LNG. In a Teorell-Stenhagen buffer (pH 5.5) containing 0.1 M NaClO4 as a supporting electrolyte, the LNG yields an irreversible well-defined oxidation peak at about 1.2 V vs. Ag/AgCl electrode. The various affecting factors, such as the pH, buffer type, supporting electrolyte, accumulation potential, scan rate and accumulation time, were tested and optimized. Also, square-wave adsorptive anodic stripping voltammetric (SWAdASV) studies show that the peak current various linearly over the LNG concentration range of 0.24 - 5.20 (ig mL−1(R2 = 0.9994). The detection and quantification limits were calculated to be 0.10 and 0.33 μg mL−1, respectively. The proposed procedure exhibits a good precision, selectivity, and stability and was applied successfully to determine the LNG in pharmaceutical formulations (tablets) and biological fluids (spiked human urine and plasma samples).

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Authors and Affiliations

  1. Chemistry Department, College of Science and Arts, Jouf University, Al-Qurayyat, 75911, Saudi Arabia

    Ahmed H. Naggar

  2. Chemistry Department, Faculty of Science, Al-Azhar University, Assiut, 71524, Egypt

    Ahmed H. Naggar

  3. Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt

    Gamal A. Saleh

  4. Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Medinah, Saudi Arabia

    Mahmoud A. Omar

  5. Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt

    Mahmoud A. Omar, Ahmed M. Haredy & Sayed M. Derayea

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  1. Ahmed H. Naggar
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  2. Gamal A. Saleh
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  3. Mahmoud A. Omar
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  4. Ahmed M. Haredy
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  5. Sayed M. Derayea
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Correspondence to Ahmed H. Naggar.

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Naggar, A.H., Saleh, G.A., Omar, M.A. et al. Square-wave Adsorptive Anodic Stripping Voltammetric Determination of Antidiabetic Drug Linagliptin in Pharmaceutical Formulations and Biological Fluids Using a Pencil Graphite Electrode. ANAL. SCI. 36, 1031–1038 (2020). https://doi.org/10.2116/analsci.19P469

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  • DOI: https://doi.org/10.2116/analsci.19P469

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