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Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish

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Abstract

Type 2 diabetes mellitus is a serious metabolic disorder in the world. Oxidative stress, as a key role on the pathogenesis of diabetes, also results in the oxidation of phospholipids. However, studies on phospholipid oxidation in diabetes, especially directly focusing on oxidized and degraded phospholipid species, are quite limited. In this study, phospholipid profiles of diabetic zebrafish plasma were characterized by LC-HRMS and MS/MS, and the total amounts of each lipid class were compared. Furthermore, the key molecular species as biomarkers in distinguishing control and diabetic samples were investigated by orthogonal partial least squares discriminant analysis. Among the identified 114 phospholipid species in total, there were 11 hydroperoxides, 7 aldehydes, and 19 lysophospholipids found significantly elevated along with the increasing blood glucose, which were known as oxidation or degradation products. Furthermore, lysophosphatidylcholine 20:5 and lysophosphatidylcholine 22:6 were assessed as potential biomarkers in diabetic zebrafish. The current work would not only help to gain further insights into diabetes, but also contribute to find new clinical parameters for the screening of the promising antioxidant agents for its therapies.

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Acknowledgments

This study was supported by (1) Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, by the Regional Innovation Strategy Support Program, Sapporo Health Innovation “Smart-H”, of the Ministry of Education, Culture, Sports, Science and Technology, Japan; and (2) JSPS KAKENHI Grant Nos. 25860294, 15K19074, and 15KK0305.

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Authors and Affiliations

  1. Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita, Sapporo, 060-0812, Japan

    Zhen Chen, Yue Wu, Rojeet Shrestha, Yaoyao Zhao, Yusuke Miura & Shu-Ping Hui

  2. Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie, 514-8507, Japan

    Liqing Zang, Hiroko Nakayama & Norihiro Nishimura

  3. Department of Integrative Pharmacology, Graduate School of Medicine, Mie University, Tsu, Mie, 514-8507, Japan

    Yasuhito Shimada

  4. Mie University Zebrafish Drug Screening Center, Tsu, Mie, 514-8507, Japan

    Yasuhito Shimada

  5. Department of Nutrition, Sapporo University of Health Sciences, Nakanuma Nishi-4-2-1-15, Higashi, Sapporo, 007-0894, Japan

    Hitoshi Chiba

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  1. Zhen Chen
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  2. Liqing Zang
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  3. Yue Wu
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Correspondence to Hitoshi Chiba or Shu-Ping Hui.

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Chen, Z., Zang, L., Wu, Y. et al. Lipidomic Profiling on Oxidized Phospholipids in Type 2 Diabetes Mellitus Model Zebrafish. ANAL. SCI. 34, 1201–1208 (2018). https://doi.org/10.2116/analsci.18P281

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  • DOI: https://doi.org/10.2116/analsci.18P281

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