Abstract
An immunosensor based on surface plasmon resonance was developed for detection of c-Kit expressed on a cell surface. The combination of the antibody solution modified with gelatin before immobilization to the sensor chip and its blocking with gelatin drastically decreased the nonspecific reaction. The condition may be useful for the detection of various cells by using antibody against cell surface marker including the c-Kit.
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Acknowledgments
The authors wish to express their appreciation to Mr. Yusui Sato and Dr. Hiroyuki Morimura for their technical support. S. Miyake was an employee of Horiba Ltd. until March 2018, and D. Irikura is an employee of Horiba Ltd., which is the manufacturer of the SPR imaging system used in this study.
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Miyake, S., Irikura, D. & Yamasaki, T. Specific Detection of c-Kit Expressed on Human Cell Surface by Immunosensor Based on Surface Plasmon Resonance. ANAL. SCI. 35, 223–225 (2019). https://doi.org/10.2116/analsci.18N022
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DOI: https://doi.org/10.2116/analsci.18N022