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Potentiometric Responses of Ionophore-Incorporated Bilayer Lipid Membranes with and without Added Anionic Sites

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Abstract

Potentiometric behaviors of bilayer lipid membranes (BLMs) incorporated with such ionophores as valinomycin, crown ethers and acyclic polyethers were described for various alkali and alkaline earth metal ions. The ionophores were directly incorporated into planar BLMs formed by the folding method. Among seven kinds of ionophores examined, only the valinomycin- and ETH 4120-based BLMs generated potentiometric Nernstian responses. However, simultaneous incorporation of anionic sites, such as sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (NaTFPB) or potassium tetrakis(4-chlorophenyl)borate (KTpClPB), was found generally effective for inducing potentiometric Nernstian responses of the ionophore-based BLMs. The added anionic sites were also found to decrease the membrane resistance, leading to improvement of probability of potential measurements, and to lower the detection limits by maintaining Nernstian slopes, thereby providing a basis for quantitative BLM sensors. By comparing the potentiometric responses of ionophore-incorporated BLMs with and without added anionic sites, the role and significance of the anionic sites in potentiometric response of BLMs based on ionophores was discussed.

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Authors and Affiliations

  1. Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, 060, Japan

    Hitoshi Sato & Mari Wakabayashi

  2. Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    Takashi Ito (Research Fellow of the Japan Society for the Promotion of Sciences (JSPS), Masao Sugawara & Yoshio Umezawa

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  1. Hitoshi Sato
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  2. Mari Wakabayashi
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  3. Takashi Ito
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  4. Masao Sugawara
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  5. Yoshio Umezawa
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Sato, H., Wakabayashi, M., Ito, T. et al. Potentiometric Responses of Ionophore-Incorporated Bilayer Lipid Membranes with and without Added Anionic Sites. ANAL. SCI. 13, 437–446 (1997). https://doi.org/10.2116/analsci.13.437

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  • DOI: https://doi.org/10.2116/analsci.13.437

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