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Effect of low modulus titanium plate fixation on rabbit femur bone healing

  • Invited Feature Paper
  • FOCUS ISSUE: Advances in Titanium Bio-Implants
  • Published:
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Abstract

The effect of a low-rigidity titanium alloy plate on bone formation during the early stage of fracture healing was investigated in this work. An artificial defect was created on rabbit femurs, and fixations of Ti–6Al–4V ELI (Ti-64) and Ti–29Nb–13Ta–4.6Zr (TNTZ) implant plates were attached to the defect with two screws at both ends of the implant plate. After 3 weeks, the rabbits were euthanized, and cross-sections of the femurs were evaluated by scanning electron microscopy, image analysis, hematoxylin–eosin staining, and Vickers hardness tests. Both fixations showed similar patterns of new bone formation in the defect area but differences in average callus area. The use of the TNTZ implant plate led to a smaller area (8.316 mm2) and harder (20 HV) callus when compared with the results of the Ti-64 implant plate (area: 18.394 mm2; callus hardness: 13 HV). We conclude that low-rigidity titanium alloys could improve bone formation during the early stages of fracture healing.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Not applicable.

References

  1. D.R. Epari, G.N. Duda, M.S. Thompson, J. Eng. Med. 224, 1543 (2010)

    Article  CAS  Google Scholar 

  2. R. Marshell, T.A. Einhorn, Injury 42, 551 (2010)

    Article  Google Scholar 

  3. S. Ghimire, S. Miramini, G. Edwards, R. Rotne, J. Xu, P. Ebeling, L. Zhanga, Bone Rep. 14, 100740 (2021)

    Article  Google Scholar 

  4. A. Vetter, Y. Liu, F. Witt, I. Manjubala, O. Sander, D.R. Epari, P. Fratzl, G.N. Duda, R. Weinkamer, J. Biomech. 44, 517 (2011)

    Article  CAS  Google Scholar 

  5. D.C. Betts, R. Müller, Front. Endocrinol. 5, 211 (2014)

    Article  Google Scholar 

  6. S. Miramini, L. Zhang, M. Richardson, M. Pirpiris, P. Mendis, K. Oloyede, G. Edwards, Methods Biomech. Biomed. Eng. 118, 900 (2015)

    Article  Google Scholar 

  7. A.D. Richard, H.F. Ellen, Drug Discov. Today 8, 59 (2003)

    Article  Google Scholar 

  8. A. Schindeler, M.M. Mcdonald, P. Bokko, D.G. Little, Semin. Cell Dev. Biol. 19, 459 (2008)

    Article  CAS  Google Scholar 

  9. J. Sonderegger, K.R. Grob, M.S. Kuster, Orthop. Rev. 2, 4 (2010)

    Article  Google Scholar 

  10. D.R. Epari, W.R. Taylor, M.O. Heller, G.N. Duda, Clin. Biomech. 21, 646 (2006)

    Article  Google Scholar 

  11. P. Kostenuik, F.M. Mirza, J. Orthop. Res. 35, 213 (2017)

    Article  Google Scholar 

  12. C.E. Baker, N.M.L. Stephanie, A.H. Alexander, S.L. Posey, J.P. Robinette, D.M. Blum, M.A. Benvenuti, H.A. Cole, S. Egawa, A. Okawa, M. Saito, J.R. McCarthy, J.S. Nyman, M. Yuasa, J.G. Schoenecker, Clin. Rev. Bone Miner. Metab. 16, 142 (2018)

    Article  Google Scholar 

  13. S. Ghimire, S. Miramini, M. Richardson, P. Mendis, L. Zhang, J. Mech. Behav. Biomed. Mater. 94, 74 (2019)

    Article  Google Scholar 

  14. S. Wolf, A. Janousek, J. Pfeil, W. Veith, F. Haas, G. Duda, L. Claes, Clin. Biomech. 13, 4 (1998)

    Article  Google Scholar 

  15. S. Miramini, L. Zhang, M. Richardson, P. Mendis, A. Oloyede, P. Ebeling, Australas. Phys. Eng. Sci. Med. 39, 123 (2016)

    Article  Google Scholar 

  16. N. Bartnikowski, L.E. Claes, L. Koval, V. Glatt, R. Bindl, R. Steck, A. Ignatius, M.A. Schuetz, D.R. Epari, Acta Ortjop. 88, 217 (2017)

    Article  Google Scholar 

  17. D.R. Epari, J.P. Kassi, H. Schell, G.N. Duda, J. Bone Joint Surg. Am. 89, 1575 (2007)

    Article  Google Scholar 

  18. M. Niinomi, Y. Liu, M. Nakai, H. Liu, H. Li, Regener. Biomater. 3, 173 (2016)

    Article  CAS  Google Scholar 

  19. M. Niinomi, M. Nakai, Int. J. Biomater. 2011, 836587 (2011)

    Article  CAS  Google Scholar 

  20. H.K. Uthoff, P. Poitras, D.S. Backman, J. Orthop. Sci. 11, 118 (2006)

    Article  Google Scholar 

  21. D.J. Lin, C.C. Chuang, J.H.C. Lin, J.W. Lee, C.P. Ju, H.S. Yin, Biomater. 28, 2582 (2007)

    Article  CAS  Google Scholar 

  22. N. Sumitomo, K. Noritake, T. Hattori, K. Morikawa, S. Niwa, K. Sato, M. Niinomi, J. Mater. Sci. Mater. Med. 19, 1581 (2008)

    Article  CAS  Google Scholar 

  23. L. Shi, L. Shi, L. Wang, Y. Duan, W. Lei, Z. Wang, J. Li, X. Fan, X. Li, S. Li, Z. Guo, PLoS ONE 8, 55015 (2013)

    Article  Google Scholar 

  24. W.H. Akeson, S.L.Y. Woo, L. Rutherford, R.D. Coutts, M. Gonsalves, D. Amiel, Acta Orthop. Scand. 47, 241 (1976)

    Article  CAS  Google Scholar 

  25. Y. Noyama, T. Miura, T. Ishimoto, T. Itaya, M. Niinomi, T. Nakano, Mater. Trans. 53, 565 (2012)

    Article  CAS  Google Scholar 

  26. S.S. Sidhu, H. Singh, M.A.H. Gepreel, J. Mater. Sci. Eng. C 121, 111661 (2021)

    Article  CAS  Google Scholar 

  27. S.D. Rogers, D.W. Howie, S.E. Graves, M.J. Pearcy, D.R. Haynes, Br. Editor. Soc. Bone Joint Surg. 79B, 311 (1997)

    Article  Google Scholar 

  28. M.A.H. Gepreel, M. Niinomi, J. Mech. Behav. Biomed. Mater. 20, 407 (2013)

    Article  Google Scholar 

  29. J.L. Domingo, Biol. Trace Elem. Res. 88, 97 (2002)

    Article  CAS  Google Scholar 

  30. I. Manjubala, Y. Liu, D.R. Epari, P. Roschger, H. Schell, P. Fratzl, G.N. Duda, Bone 45, 185 (2009)

    Article  CAS  Google Scholar 

  31. Y. Liu, I. Manjubala, H. Schell, D.R. Epari, P. Roschger, G.N. Duda, P. Fratzl, J. Bone Miner. Res. 25, 2029 (2010)

    Article  Google Scholar 

  32. L. Claes, J. Orthop. Trauma 25, S4 (2011)

    Article  Google Scholar 

  33. T. Yamaji, K. Ando, S. Wolf, P. Augat, L. Claes, J. Orthop. Sci. 6, 571 (2001)

    Article  CAS  Google Scholar 

  34. T.J. Lujan, C.E. Henderson, S.M. Madey, D.C. Fitzpatrick, J.L. Marsh, M. Bottlang, J. Orthop, Trauma 24, 156 (2010)

    Google Scholar 

  35. R.M. Hoerth, M. Kerschnitzki, M. Aido, I. Schmidt, M. Burghammer, G.N. Duda, P. Fratzl, B.M. Willie, W. Wagermaier, J. Mech. Behav. Biomed. Mater. 77, 258 (2018)

    Article  Google Scholar 

  36. P.L. Leong, E.F. Morgan, Acta Biomater. 4, 1569 (2008)

    Article  CAS  Google Scholar 

  37. M.L. Angelescu, A. Dan, E. Ungureanu, N.Z. Ivan, B.M. Galbinasu, Metals 12, 248 (2022)

    Article  CAS  Google Scholar 

  38. D. Kent, G. Wang, M. Dargusch, J. Mech. Behav. Biomed. Mater. 28, 15 (2013)

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Mr. Katsumi Okumoto from the Life Science Research Center of Kindai University for his assistance in the sample preparation procedures and H&E staining experiments.

Funding

This work was supported by JSPS KAKENHI Grant No. JP20H02036.

Author information

Authors and Affiliations

  1. Department of Mechanical Engineering, Kindai University, 3-4-1, Kowakae, , Higashiōsaka, Osaka, 577-8502, Japan

    Norain Binti Abdullah, Daisuke Miyazaki, Kosuke Ueki & Masaaki Nakai

  2. Department of Biomedical Engineering, Kindai University, 689-10 Nishimitani, Kinokawa, Wakayama, 649-6493, Japan

    Ei Yamamoto

Authors
  1. Norain Binti Abdullah
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  2. Daisuke Miyazaki
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  3. Ei Yamamoto
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  4. Kosuke Ueki
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  5. Masaaki Nakai
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Corresponding author

Correspondence to Masaaki Nakai.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

All animal experimental procedures were approved by the Animal Experiment Committee of the Faculty of Biology-Oriented Science and Technology, Kindai University.

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Mathias Goeken and Benoit Merle were editors of this journal during the review and decision stage. For the JMR policy on review and publication of manuscripts authored by editors, please refer to http://www.mrs.org/editor-manuscripts/

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Abdullah, N.B., Miyazaki, D., Yamamoto, E. et al. Effect of low modulus titanium plate fixation on rabbit femur bone healing. Journal of Materials Research 37, 2536–2545 (2022). https://doi.org/10.1557/s43578-022-00616-z

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  • DOI: https://doi.org/10.1557/s43578-022-00616-z

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