Skip to main content
SpringerLink
Log in

Peptide-Enhanced Nucleic Acid Delivery

  • Technical Feature
  • Published:
MRS Bulletin Aims and scope Submit manuscript

Abstract

Numerous barriers, both extracellular and intracellular, hinder successful and efficient nonviral nucleic acid delivery. Due to their small size and ability to specifically recognize and interact with molecular targets, peptides can be incorporated as modular elements into synthetic nucleic acid delivery systems to overcome many of these barriers. Three classes of peptides that have frequently been integrated as components in nucleic acid delivery systems include cell-penetrating peptides (CPPs), endosomal release peptides, and nuclear localization sequences (NLSs).Various additional classes of peptides show promise for enhancing nucleic acid delivery by targeting cell surface receptors, inhibiting nuclease activity, and directing nucleic acids toward intracellular targets. In addition to a review of the various existing approaches to peptide-enhanced nucleic acid delivery, this article will discuss strategies for the development of new peptides and approaches for the incorporation of these peptides into nucleic acid delivery systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. B. Gupta, T.S. Levchenko, V.P. Torchilin, Adv. Drug Deliv. Rev. 57 (2005) p. 637.

    Article  CAS  Google Scholar 

  2. P. Jarver and U. Langel, Drug Discov. Today 9 (2004) p. 395.

    Article  CAS  Google Scholar 

  3. P. Lundberg and U. Langel, J. Mol. Recognit. 16 (2003) p. 227.

    Article  CAS  Google Scholar 

  4. J. Temsamani and P. Vidal, Drug Discov. Today 9 (2004) p. 1012.

    Article  CAS  Google Scholar 

  5. A. Muratovska and M.R. Eccles, FEBS Lett. 558 (2004) p. 63.

    Article  CAS  Google Scholar 

  6. D. Derossi, S. Calvet, A. Trembleau, A. Brunissen, G. Chassaing, and A. Prochiantz, J. Biol. Chem. 271 (1996) p. 18188.

    Article  CAS  Google Scholar 

  7. E. Vives, P. Brodin, and B. Lebleu, J. Biol. Chem. 272 (1997) p. 16010.

    Article  CAS  Google Scholar 

  8. J.P. Richard, K. Melikov, E. Vives, C. Ramos, B. Verbeure, M.J. Gait, L.V. Chernomordik, and B. Lebleu, J. Biol. Chem. 278 (2003) p. 585.

    Article  CAS  Google Scholar 

  9. N.J. Caron, S.P. Quenneville, and J.P. Tremblay, Biochem. Biophys. Res. Commun. 319 (2004) p. 12.

    Article  CAS  Google Scholar 

  10. S. Console, C. Marty, C. Garcia-Echeverria, R. Schwendener, and K. Ballmer-Hofer, J. Biol. Chem. 278 (2003) p. 35109.

    Article  CAS  Google Scholar 

  11. R. Fischer, K. Kohler, M. Fotin-Mleczek, and R. Brock, J. Biol. Chem. 279 (2004) p. 12625.

    Article  CAS  Google Scholar 

  12. T.B. Potocky, A.K. Menon, and S.H. Gellman, J. Biol. Chem. 278 (2003) p. 50188.

    Article  CAS  Google Scholar 

  13. M.F. Ross, A. Filipovska, R.A. Smith, M.J. Gait, and M.P. Murphy, Biochem. J. 383 (2004) p. 457.

    Article  CAS  Google Scholar 

  14. A. Ziegler, P. Nervi, M. Durrenberger, and J. Seelig, Biochemistry 44 (2005) p. 138.

    Article  CAS  Google Scholar 

  15. H. Brooks, B. Lebleu, and E. Vives, Adv. Drug Deliv. Rev. 57 (2005) p. 559.

    Article  CAS  Google Scholar 

  16. A. Astriab-Fisher, D.S. Sergueev, M. Fisher, B.R. Shaw, and R.L. Juliano, Biochem. Pharmacol. 60 (2000) p. 83.

    Article  CAS  Google Scholar 

  17. I.A. Ignatovich, E.B. Dizhe, A.V. Pavlotskaya, B.N. Akifiev, S.V. Burov, S.V. Orlov, and A.P. Perevozchikov, J. Biol. Chem. 278 (2003) p. 42625.

    Article  CAS  Google Scholar 

  18. Z. Liu, M. Li, D. Cui, and J. Fei, J. Controlled Release 102 (2005) p. 699.

    Article  CAS  Google Scholar 

  19. C. Rudolph, C. Plank, J. Lausier, U. Schillinger, R.H. Muller, and J. Rosenecker, J. Biol. Chem. 278 (2003) p. 11411.

    Article  CAS  Google Scholar 

  20. V.P. Torchilin, T.S. Levchenko, R. Rammohan, N. Volodina, B. Papahadjopoulos-Sternberg, and G.G. D’Souza, Proc. Natl. Acad. Sci. USA 100 (2003) p. 1972.

    Article  CAS  Google Scholar 

  21. C.H. Tung, S. Mueller, and R. Weissleder, Bioorg. Med. Chem. 10 (2002) p. 3609.

    Article  CAS  Google Scholar 

  22. B. Allinquant, P. Hantraye, P. Mailleux, K. Moya, C. Bouillot, and A. Prochiantz, J. Cell Biol. 128 (1995) p. 919.

    Article  CAS  Google Scholar 

  23. M. Pooga, U. Soomets, M. Hallbrink, A. Valkna, K. Saar, K. Rezaei, U. Kahl, J.X. Hao, X.J. Xu, Z. Wiesenfeld-Hallin, T. Hokfelt, T. Bartfai, and U. Langel, Nat. Biotechnol. 16 (1998) p. 857.

    Article  CAS  Google Scholar 

  24. C.M. Troy, D. Derossi, A. Prochiantz, L.A. Greene, and M.L. Shelanski, J. Neurosci. 16 (1996) p. 253.

    Article  CAS  Google Scholar 

  25. C.G. Ostenson, A.C. Sandberg-Nordqvist, J. Chen, M. Hallbrink, D. Rotin, U. Langel, S. Efendic, Biochem. Biophys. Res. Commun. 291 (2002) p. 945.

    Article  CAS  Google Scholar 

  26. S. Futaki, W. Ohashi, T. Suzuki, M. Niwa, S. Tanaka, K. Ueda, H. Harashima, and Y. Sugiura, Bioconjugate Chem. 12 (2001) p. 1005.

    Article  CAS  Google Scholar 

  27. Z. Siprashvili, F.A. Scholl, S.F. Oliver, A. Adams, C.H. Contag, P.A. Wender, and P.A. Khavari, Hum. Gene Ther. 14 (2003) p. 1225.

    Article  CAS  Google Scholar 

  28. S.M. van Rossenberg, A.C. van Keulen, J.W. Drijfhout, S. Vasto, H.K. Koerten, F. Spies, J.M. van‘t Noordende, T.J. van Berkel, and E.A. Biessen, Gene Ther. 11 (2004) p. 457.

    Article  CAS  Google Scholar 

  29. O. Boussif, F. Lezoualc’h, M.A. Zanta, M.D. Mergny, D. Scherman, B. Demeneix, and J.P. Behr, Proc. Natl. Acad. Sci. USA 92 (1995) p. 7297.

    Article  CAS  Google Scholar 

  30. D.W. Pack, D. Putnam, and R. Langer, Biotechnol. Bioeng. 67 (2000) p. 217.

    Article  CAS  Google Scholar 

  31. C. Pichon, M.B. Roufai, M. Monsigny, and P. Midoux, Nucleic Acids Res. 28 (2000) p. 504.

    Article  CAS  Google Scholar 

  32. D. Putnam, C.A. Gentry, D.W. Pack, and R. Langer, Proc. Natl. Acad. Sci. USA 98 (2001) p. 1200.

    Article  CAS  Google Scholar 

  33. J. Zabner, A.J. Fasbender, T. Moninger, K.A. Poellinger, and M.J. Welsh, J. Biol. Chem. 270 (1995) p. 18997.

    Article  CAS  Google Scholar 

  34. J. Haensler and F.C. Szoka Jr., Bioconjugate Chem. 4 (1993) p. 372.

    Article  CAS  Google Scholar 

  35. R.A. Parente, L. Nadasdi, N.K. Subbarao, and F.C. Szoka Jr., Biochemistry 29 (1990) p. 8713.

    Article  CAS  Google Scholar 

  36. W. Li, F. Nicol, and F.C. Szoka Jr., Adv. Drug Deliv. Rev. 56 (2004) p. 967.

    Article  CAS  Google Scholar 

  37. D.H. Haas and R.M. Murphy, J. Pept. Res. 63 (2004) p. 9.

    Article  CAS  Google Scholar 

  38. H. Lee, J.H. Jeong, and T.G. Park, J. Controlled Release 79 (2002) p. 283.

    Article  CAS  Google Scholar 

  39. A. Kichler, K. Mechtler, J.P. Behr, and E. Wagner, Bioconjugate Chem. 8 (1997) p. 213.

    Article  CAS  Google Scholar 

  40. A. Subramanian, H. Ma, K.N. Dahl, J. Zhu, and S.L. Diamond, J. Gene Med. 4 (2002) p. 75.

    Article  Google Scholar 

  41. E. Wagner, C. Plank, K. Zatloukal, M. Cotten, and M.L. Birnstiel, Proc. Natl. Acad. Sci. USA 89 (1992) p. 7934.

    Article  CAS  Google Scholar 

  42. M.R. Capecchi, Cell 22 (1980) p. 479.

    Article  CAS  Google Scholar 

  43. S. Brunner, T. Sauer, S. Carotta, M. Cotten, M. Saltik, and E. Wagner, Gene Ther. 7 (2000) p. 401.

    Article  CAS  Google Scholar 

  44. R. Cartier and R. Reszka, Gene Ther. 9 (2002) p. 157.

    Article  CAS  Google Scholar 

  45. V. Escriou, M. Carriere, D. Scherman, and P. Wils, Adv. Drug Deliv. Rev. 55 (2003) p. 295.

    Article  CAS  Google Scholar 

  46. E. Hebert, Biol. Cell 95 (2003) p. 59.

    Article  CAS  Google Scholar 

  47. F.M. Munkonge, D.A. Dean, E. Hillery, U. Griesenbach, and E.W. Alton, Adv. Drug Deliv. Rev. 55 (2003) p. 749.

    Article  CAS  Google Scholar 

  48. M. Nakanishi, T. Akuta, E. Nagoshi, A. Eguchi, H. Mizuguchi, and T. Senda, Eur. J. Pharm. Sci. 13 (2001) p. 17.

    Article  CAS  Google Scholar 

  49. M.A. Zanta, P. Belguise-Valladier, and J.P. Behr, Proc. Natl. Acad. Sci. USA 96 (1999) p. 91.

    Article  CAS  Google Scholar 

  50. A. Subramanian, P. Ranganathan, and S.L. Diamond, Nat. Biotechnol. 17 (1999) p. 873.

    Article  CAS  Google Scholar 

  51. T. Nagasaki, T. Myohoji, T. Tachibana, S. Futaki, and S. Tamagaki, Bioconjugate Chem. 14 (2003) p. 282.

    Article  CAS  Google Scholar 

  52. M. Tanimoto, H. Kamiya, N. Minakawa, A. Matsuda, and H. Harashima, Bioconjugate Chem. 14 (2003) p. 1197.

    Article  CAS  Google Scholar 

  53. M.A. van der Aa, G.A. Koning, C. d’Oliveira, R.S. Oosting, K.J. Wilschut, W.E. Hennink, and D.J. Crommelin, J. Gene Med. 7 (2005) p. 208.

    Article  CAS  Google Scholar 

  54. J.J. Ludtke, G. Zhang, M.G. Sebestyen, and J.A. Wolff, J. Cell Sci. 112 (Pt. 12) (1999) p. 2033.

    CAS  Google Scholar 

  55. N. Pante and M. Kann, Mol Biol. Cell 13 (2002) p. 425.

    Article  CAS  Google Scholar 

  56. K.H. Bremner, L.W. Seymour, A. Logan, and M.L. Read, Bioconjugate Chem. 15 (2004) p. 152.

    Article  CAS  Google Scholar 

  57. L.J. Branden, A.J. Mohamed, and C.I. Smith, Nat. Biotechnol. 17 (1999) p. 784.

    Article  CAS  Google Scholar 

  58. C.K. Chan and D.A. Jans, Hum Gene Ther. 10 (1999) p. 1695.

    Article  CAS  Google Scholar 

  59. C.K. Chan and D.A. Jans, Gene Ther. 8 (2001) p. 166.

    Article  CAS  Google Scholar 

  60. C.K. Chan, T. Senden, D.A. Jans, Gene Ther. 7 (2000) p. 1690.

    Article  CAS  Google Scholar 

  61. C. Ciolina, G. Byk, F. Blanche, V. Thuillier, D. Scherman, and P. Wils, Bioconjugate Chem. 10 (1999) p. 49.

    Article  CAS  Google Scholar 

  62. P. Collas, H. Husebye, and P. Alestrom, Transgenic Res. 5 (1996) p. 451.

    Article  CAS  Google Scholar 

  63. C. Neves, V. Escriou, G. Byk, D. Scherman, and P. Wils, Cell Biol. Toxicol. 15 (1999) p. 193.

    Article  CAS  Google Scholar 

  64. W. Ritter, C. Plank, J. Lausier, C. Rudolph, D. Zink, D. Reinhardt, and J. Rosenecker, J. Mol. Med. 81 (2003) p. 708.

    Article  CAS  Google Scholar 

  65. B. Schwartz, M.A. Ivanov, B. Pitard, V. Escriou, R. Rangara, G. Byk, P. Wils, J. Crouzet, and D. Scherman, Gene Ther. 6 (1999) p. 282.

    Article  CAS  Google Scholar 

  66. M.G. Sebestyen, J.J. Ludtke, M.C. Bassik, G. Zhang, V. Budker, E.A. Lukhtanov, J.E. Hagstrom, and J.A. Wolff, Nat. Biotechnol. 16 (1998) p. 80.

    Article  CAS  Google Scholar 

  67. Y. Jenkins, M. McEntee, K. Weis, and W.C. Greene, J. Cell Biol. 143 (1998) p. 875.

    Article  CAS  Google Scholar 

  68. F. Zhang, P. Andreassen, P. Fender, E. Geissler, J.F. Hernandez, and J. Chroboczek, Gene Ther. 6 (1999) p. 171.

    Article  CAS  Google Scholar 

  69. M. Carriere, V. Escriou, A. Savarin, and D. Scherman, BMC Biotechnol. 3 (2003) p. 14.

    Article  Google Scholar 

  70. K.A. Mislick and J.D. Baldeschwieler, Proc. Natl. Acad. Sci. USA 93 (1996) p. 12349.

    Article  CAS  Google Scholar 

  71. M. Colin, M. Maurice, G. Trugnan, M. Kornprobst, R.P. Harbottle, A. Knight, R.G. Cooper, A.D. Miller, J. Capeau, C. Coutelle, and M.C. Brahimi-Horn, Gene Ther. 7 (2000) p. 139.

    Article  CAS  Google Scholar 

  72. R.P. Harbottle, R.G. Cooper, S.L. Hart, A. Ladhoff, T. McKay, A.M. Knight, E. Wagner, A.D. Miller, and C. Coutelle, Hum. Gene Ther. 9 (1998) p. 1037.

    Article  CAS  Google Scholar 

  73. R. Pasqualini, E. Koivunen, and E. Ruoslahti, Nat. Biotechnol. 15 (1997) p. 542.

    Article  CAS  Google Scholar 

  74. S. Moffatt, S. Wiehle, and R.J. Cristiano, Hum. Gene Ther. 16 (2005) p. 57.

    Article  CAS  Google Scholar 

  75. M.J. Writer, B. Marshall, M.A. Pilkington-Miksa, S.E. Barker, M. Jacobsen, A. Kritz, P.C. Bell, D.H. Lester, A.B. Tabor, H.C. Hailes, N. Klein, and S.L. Hart, J. Drug Target 12 (2004) p. 185.

    Article  CAS  Google Scholar 

  76. S.A. Nicklin, S.J. White, S.J. Watkins, R.E. Hawkins, and A.H. Baker, Circulation 102 (2000) p. 231.

    Article  CAS  Google Scholar 

  77. K. Porkka, P. Laakkonen, J.A. Hoffman, M. Bernasconi, and E. Ruoslahti, Proc. Natl. Acad. Sci. USA 99 (2002) p. 7444.

    Article  CAS  Google Scholar 

  78. P. Laakkonen, K. Porkka, J.A. Hoffman, and E. Ruoslahti, Nat. Med. 8 (2002) p. 751.

    Article  CAS  Google Scholar 

  79. P.M. Smith, G.F. Ross, R.W. Taylor, D.M. Turnbull, and R.N. Lightowlers, Biochim. Biophys. Acta 1659 (2004) p. 232.

    Article  CAS  Google Scholar 

  80. P. Seibel, J. Trappe, G. Villani, T. Klopstock, S. Papa, and H. Reichmann, Nucleic Acids Res. 23 (1995) p. 10.

    Article  CAS  Google Scholar 

  81. G.G. D’Souza, R. Rammohan, S.M. Cheng, V.P. Torchilin, and V. Weissig, J. Controlled Release 92 (2003) p. 189.

    Article  CAS  Google Scholar 

  82. G. Manfredi, J. Fu, J. Ojaimi, J.E. Sadlock, J.Q. Kwong, J. Guy, and E.A. Schon, Nat. Genet. 30 (2002) p. 394.

    Article  CAS  Google Scholar 

  83. M. Pooga, M. Hallbrink, M. Zorko, and U. Langel, FASEB J. 12 (1998) p. 67.

    Article  CAS  Google Scholar 

  84. M.C. Morris, P. Vidal, L. Chaloin, F. Heitz, and G. Divita, Nucleic Acids Res. 25 (1997) p. 2730.

    Article  CAS  Google Scholar 

  85. M.C. Morris, L. Chaloin, J. Mery, F. Heitz, and G. Divita, Nucleic Acids Res. 27 (1999) p. 3510.

    Article  CAS  Google Scholar 

  86. F. Maruta, A.L. Parker, K.D. Fisher, P.G. Murray, D.J. Kerr, and L.W. Seymour, J. Drug Target 11 (2003) p. 53.

    Article  CAS  Google Scholar 

  87. T. Mori, Curr. Pharm. Des. 10 (2004) p. 2335.

    Article  CAS  Google Scholar 

  88. J.J. Sperinde, S.J. Choi, and F.C. Szoka Jr., J. Gene Med. 3 (2001) p. 101.

    Article  CAS  Google Scholar 

  89. M. Morpurgo, M. Kirschner, and A. Radu, J. Biochem. Biophys. Methods 52 (2002) p. 31.

    Article  CAS  Google Scholar 

  90. C. Pinilla, J.R. Appel, E. Borras, and R.A. Houghten, Nat. Med. 9 (2003) p. 118.

    Article  CAS  Google Scholar 

  91. O.H. Aina, T.C. Sroka, M.L. Chen, and K.S. Lam, Biopolymers 66 (2002) p. 184.

    Article  CAS  Google Scholar 

  92. M. Isalan, M.I. Santori, C. Gonzalez, and L. Serrano, Nat. Methods 2 (2005) p. 113.

    Article  CAS  Google Scholar 

  93. C. Neves, G. Byk, D. Scherman, and P. Wils, FEBS Lett. 453 (1999) p. 41.

    Article  CAS  Google Scholar 

  94. O. Zelphati, X. Liang, C. Nguyen, S. Barlow, S. Sheng, Z. Shao, and P.L. Felgner, Biotechniques 28 (2000) pp. 304, 312, 316.

    Article  CAS  Google Scholar 

Download references

Authors
  1. Jamie M. Bergen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Suzie H. Pun
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bergen, J.M., Pun, S.H. Peptide-Enhanced Nucleic Acid Delivery. MRS Bulletin 30, 663–667 (2005). https://doi.org/10.1557/mrs2005.194

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1557/mrs2005.194

Keywords

Search

Navigation