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A Simple and Reliable Method for Quantification of Glucosamine in Nutritional Supplements

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Summary

HPTLC on amino plates, with simple heating of the plates for derivatization, has been used for quantification of glucosamine in nutritional supplements. On heating the plate glucosamine reacts to form a compound which strongly absorbs light between 305 and 330 nm, with weak fluorescence. The reaction product can be detected sensitively either by absorption of light or by fluorescence detection. The detection limit in absorption mode is approximately 25 ng per spot. In fluorescence mode a detection limit of 15 ng is achievable. A calibration plot for absorption detection is linear in the range 25 to 4000 ng glucosamine. The derivative formed from glucosamine by heating is stable for months, and the relative standard deviation is 1.64% for 600 ng glucosamine. The amounts of glucosamine found in nutritional supplements were in agreement with the label declarations.

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Authors and Affiliations

  1. University of Applied Sciences Offenburg, Badstrasse 24, 77652, Offenburg, Germany

    Michaela Bleichert & Bernd Spangenberg

  2. University of Applied Sciences Gießen-Friedberg, Wilhelm-Leuschner-Strasse 13, 61169, Friedberg, Germany

    Hanns-Simon Eckhardt & Karl-Friedrich Klein

Authors
  1. Michaela Bleichert
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  2. Hanns-Simon Eckhardt
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  3. Karl-Friedrich Klein
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  4. Bernd Spangenberg
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Correspondence to Bernd Spangenberg.

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Bleichert, M., Eckhardt, HS., Klein, KF. et al. A Simple and Reliable Method for Quantification of Glucosamine in Nutritional Supplements. JPC-J Planar Chromat 21, 55–59 (2008). https://doi.org/10.1556/JPC.2007.6005

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  • DOI: https://doi.org/10.1556/JPC.2007.6005

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