Abstract
The variability of microsatellite markers of 16 genotypes of triticale (× Triticosecale Wittmack, 2n = 6x = 42, BBAARR) was studied. Five varieties from Poland (Gutek, Kitaro, Lamberto, Presto and Tornado), three from Germany (Lupus, Ticino and Triamant), one from Russia (Valentin-90) and seven translocation forms derived from cv. Presto (donors of good bread-making quality) were analysed. SSR markers localised on chromosomes of the A, B, D and R genomes were chosen from literature for analysis. Based on 48 SSR markers (27 wheat and 21 rye SSR markers) a dendrogram was calculated, which highly significantly differentiated the Valentine-90 genotype from all the other 15 genotypes split into three sub-clusters. The first one includes the cv. Gutek, Tornado, Presto and translocation forms of cv. Presto. The second sub-cluster consists of the cv. Kitaro, Lamberto, Ticino and Triamant. The third sub-cluster cluster consists of the cv. Lupus only. The diversity index (DI), the probabilities of identity (PI) and the polymorphic information content (PIC) of SSR markers were calculated. We detected 184 alleles from 48 markers with an average of 3.83 alleles per locus (ranging from 1 to 9 alleles per locus). The average polymorphic information content was 0.48 ranging between 0.00 and 0.85.
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Bohn, F., Utz, H.F., Melchinger, A.E. 1999. Genetic similarities among winter wheat cultivars determined on the basis of RFLPs, AFLPs, and SSRs and their use for predicting progeny variance. Crop Sci. 39:228–237.
Brantestam Kolodinska, A., von Bothmer, R., Dayteg, C., Rashai, I., Weibull, J. 2007. Genetic diversity changes and relationships in spring barley (Hordeum vulgare L.) germplasm of Nordic and Baltic areas as shown by SSR markers. Genet. Resour. Crop Evol. 54:749–758.
Devos, K.M., Bryan, G.J., Collins, A.J., Stephenson, P., Gale, M.D. 1995. Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor. Appl. Genet. 90:247–252.
Giunta, F., Motzo, R. 2004. Sowing rate and cultivar affect total biomass and grain yield of spring triticale (× Triticosecale Wittmack) grain in a Mediterranean-type environment. Field Crops Res. 87:193–197.
Gregáňová, Z., Kraic, J., Gálová, Z. 2005. Effectiveness of microsatellites in differentiation of elite wheat cultivars. Biologia 60:665–670.
Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bulletin del la Société Vaudoise des Sciences Naturelles 44:223–270.
Jones, E.S., Sullivan, H., Bhattramakki, D., Smith, J.S.C. 2007. A comparison of simple sequence repeat and single nucleotide polymorphism marker technologies for the genotypic analysis of maize (Zea mays L.). Theor. Appl. Genet. 115:361–371.
Khlestkina, E.K., Than, M.H.M., Pestsova, E.G., Röder, M.S., Malyshev, S.V., Korzun, V., Börner, A. 2004. Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequences tags. Theor. Appl. Genet. 109:725–732.
Kuelung, C., Baezinger, P.S., Dweikat, I. 2004. Transferability of SSR markers among wheat, rye and triticale. Theor. Appl. Genet. 108:1147–1150.
Kuelung, C., Baenziger, P.S., Kachman, S.D., Dweikat, I. 2006. Evaluating the genetic diversity of triticale with wheat and rye SSR markers. Crop Sci. 46:1692–1700.
Lukaszewski, A.J. 2006. Cytogenetically engineered rye chromosomes 1R to improve bread-making quality of hexaploid triticale. Crop Sci. 46:2183–2194.
Manifesto, M.M., Schlatter, A.R., Hopp, H.E., Suarez, E.Y., Dubcovsky, J. 2001. Quantitative evalution of genetic diversity in wheat germplasm using molecular markers. Crop Sci. 41:682–690.
Mantovani, P., van der Linden, G., Maccaferri, M., Sanguineti, M.C., Tuberosa, R. 2006. Nucleotide-binding site (NBS) profiling of genetic diversity in durum wheat. Genome 49:1473–1480.
Martinek, P., Vinterová, M., Burešová, I., Vyhnánek, T. 2008. Agronomic and quality characteristics of triticale (× Triticosecale Wittmack) with HMW glutenin subunits 5+10. J. Cereal Sci. 47:68–78.
Röder, M.S., Korzun, V., Wendehake, K., Plaschke, J., Tixier, M.H., Leroy, P., Ganal, M.W. 1998. A microsatellite map of wheat. Genetics 149:2007–2023.
Russell, J., Fuller, J., Young, G., Thomas, B., Taramino, G., Macaulay, M., Waugh, R., Powell, W. 1997. Discriminating between barley genotypes using microsatellite markers. Genome 40:442–450.
Somers, D.J., Isaac, P., Edwards, K. 2004. A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.
Tams, S.H., Bauer, E., Oettler, G., Melchinger, A.E. 2004. Genetic diversity in European winter triticale determined with SSR markers and coancestry coefficient. Theor. Appl. Genet. 108:1385–1391.
Tams, S.H., Melchinger, A.E., Bauer, E. 2005. Genetic similarity among European winter triticale elite germplasms assessed with AFLP and comparisons with SSR and pedigree data. Plant Breed. 124:154–160.
Vyhnánek, T., Bednár, J. 2003. Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker. Plant Soil Environ. 49:95–98.
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Vyhnánek, T., Nevrtalová, E. & Slezáková, K. Detection of the Genetic Variability of Triticale Using Wheat and Rye SSR Markers. CEREAL RESEARCH COMMUNICATIONS 37, 23–29 (2009). https://doi.org/10.1556/CRC.37.2009.1.3
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DOI: https://doi.org/10.1556/CRC.37.2009.1.3