Summary
The powdery mildew resistance gene Pm2 is effective in China. Bulked segregant analysis (BSA) was used to search for microsatellite markers linked to Pm2. Twenty-one microsatellite primer pairs located on chromosome 5DS were screened; three polymorphic loci Xcfd81-5DS, Xgwm190-5DS, and Xcfd18-5DS were linked to Pm2 using an F2 population from Chinese Spring × C114118 (with Pm2) consisting of 814 individuals. The genetic distances between Pm2 and the three markers were: 2.0cM, 34.2cM and 44.2cM, respectively. Microsatellite marker Xcfd81-5DS could be used in marker assisted selection for Pm2 provided any chosen Pm2 source also carries the relevant marker.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Briggle L.W. (1969): Near-isogenic lines of wheat with genes for resistance to Erysiphe graminis f. sp. tritici. Crop Sci. 9:70–72.
Bryan G.J., Collins A.J., Stephenson P., Orry A., Smith J.B., Gale M.D. (1997): Isolation and characterization of microsatellites from hexaploid bread wheat. Theor. Appl. Genet. 94:557–563.
Hartl L., Weiss H., Stephan U., Zeller F.J., Jahoor A. (1995): Molecular identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). Theor. Appl. Genet. 90:601–606.
Heun M., Fischbeck G. (1987): Gene for powdery mildew resistance in cultivars for spring wheat. Plant Breeding 99:282–288.
Hsam S.L.K., Lapochkina I.F., Zeller F.J. (2003): Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica 133:367–370.
Huang X.Q., Hsam S.L.K., Zeller F.J. (1997): Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.) IX. Cultivars, landraces and breeding lines grown in China. Plant Breeding 116:233–238.
Lincoln S., Daly, M., Lander, E. (1992): Construction of genetic maps with MAPMAKER/EXP3.0, Ed. 3. Whitehead Institute Technical Report, Whitehead Institute, Cambridge, MA.
Liu J., Liu D., Tao W., Li W., Wang S., Chen P., Cheng S., Gao D. (2000): Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breeding 119:21–24.
Lutz J., Limpert E., Bartos P., Zeller F.J. (1992): Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). I. Czechoslovakian cultivars. Plant Breeding 108:33–39.
Ma Z.Q., Sorrells M.E., Tanksley S.D. (1994): RFLP markers linked to powdery mildew resistance genes Pm1, Pm2, Pm3 and Pm4 in wheat. Genome 37:871–875.
McIntosh R.A., Baker E.P. (1970): Cytogenetical studies in wheat. IV. Chromosome location and linkage studies involving the Pm2 locus for powdery mildew resistance. Euphytica 19:71–77.
McIntosh R.A., Devos K.M., Dubcovsky J., Morris C.F., Rogers W.J. (2003): Catalogue of gene symbols for wheat: 2003 supplement. http://wheat.pw.usda.gov/ggpages/wgc/2003upd.html
Mohler V., Jahoor A. (1996): Allele specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals. Theor. Appl. Genet. 93:1078–1082.
Paillard S., Schnurbusch T., Winzeler M., Messmer M., Sourdille P., Abderhalden O., Keller B., Schachermayr G.. (2003): An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theor. Appl. Genet. 107:1235–1242.
Plaschke J., Ganal M.W., Röder M.S. (1995): Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor. Appl. Genet. 91:1001–1007.
Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. (1998): A microsatellite map of wheat. Genetics 149:2007–2023.
Sharp P.J., Kreis M., Shewry P.R., Gale M.D. (1988): Location of β-amylase sequence in wheat and its relatives. Theor. Appl. Genet. 75:289–290.
Somers D.J., Isaac P., Edwards K. (2004): A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.
Sourdille P., Singh D., Cadalen T., Brown-Guedira G.L., Gay G., Qi L.L., Gill B.S., Dufour P., Murigneux A., Bernard M. (2004): Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Funct Integr Genomics 4:12–25.
Zeller F.J., Lutz J., Reimlein E.I., Limpert E., Koening J. (1993): Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). II. French cultivars. Agronomie 13:201–207.
Zhu Z.D., Zhou R.H., Kong X.Y., Dong Y.C., Jia J.Z. (2005): Microsatellite markers linked to two powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat. Genome 48:585–590.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Qiu, Y.C., Sun, X.L., Zhou, R.H. et al. Identification of microsatellite markers linked to powdery mildew resistance gene Pm2 in wheat. CEREAL RESEARCH COMMUNICATIONS 34, 1267–1273 (2006). https://doi.org/10.1556/CRC.34.2006.4.268
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.34.2006.4.268