Abstract
Wheat continues to be one of the most cultivated cereals in the world, and also in Romania. Leaf rust caused by Puccinia triticina reduces the wheat yield and grains quality worldwide. In the context of climate change, leaf rust has become a more important problem for both wheat growers and breeders in our country. Use of genetic resources, carrying rust resistance genes, play an important role in breeding programs leading to resistant varieties, which can have positive impact on environment and economy. Therefore, the identification of resistance genes in modern wheat cultivars and breeding lines, and then selection of the best resistance genes combination(s) are the first steps for a successful breeding program. At present, one of the best known and studied adult plant leaf rust resistance gene is Lr34 that contributes significantly to durable leaf rust resistance. The functional markers that enable early detection of this gene are a major advantage in the wheat breeding.
The aim of this study was to evaluate the presence of the slow rusting resistance gene Lr34 in Romanian wheat germplasm, using cssfr4 and cssfr5 molecular markers. Screening of 47 winter bread wheat cultivars and 47 breeding lines with these markers showed the presence of the Lr34 resistant haplotype in 62% (homozygous genotypes) of the total genotypes. A high frequency (79%) of Lr34 resistance allele was found among 47 breeding lines, suggesting that maintenance of a high frequency of this allele represents a real advantage for the development of adult plant resistance in Romanian breeding programs.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anonymous 2014. Producoia agricola vegetala la principalele culturi, în anul 2013 (Agricultural production of main crops in 2013). https://doi.org/www.insse.ro/cms/files/statistici/comunicate/com_anuale/Prod_veg/prod_veg_r13 (in Romanian)
Dakouri, A., McCallum, B.D., Walichnowski, A.Z., Cloutier, S. 2010. Fine-mapping of the leaf rust Lr34 locus in Triticum aestivum (L.) and characterization of large germplasm collections support the ABC transporter as essential for gene function. Theor. Appl. Genet. 121:373–384.
Dakouri, A., McCallum, B.D., Radovanovic, N., Cloutier, S. 2013. Molecular and phenotypic characterization of seedling and adult plant leaf rust resistance in a world wheat collection. Mol. Breeding 32:663–677.
Dyck, P.L. 1977. Genetics of leaf rust reaction in three introductions of common wheat. Can. J. Genet. Cytol. 19:711–716.
Dyck, P.L. 1987. The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469.
German, S.E., Kolmer, J.A. 1992. Effect of gene Lr34 in the enhancement of resistance to leaf rust of wheat. Theor. Appl. Genet. 84:97–105.
Herrera-Foessel, S.A., Lagudah, E.S., Huerta-Espino, J., Hayden, M.J., Bariana, H.S., Singh, D., Singh, R.P. 2011. New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor. Appl. Genet. 122:239–249.
Herrera-Foessel, S.A., Singh, R.P., Huerta-Espino, J., Rosewarne, G.M., Periyannan, S.K., Viccar, L., Calvo-Salazar, V., Lan, C., Lagudah, E.S. 2012. Lr68: A new gene conferring slow rusting resistance to leaf rust in wheat. Theor. Appl. Genet. 124:1475–1486.
Hiebert, C.W., Thomas, J.B., McCallum, B.D., Humphreys, G.D., DePauw, R.M., Hayden, M.J., Mago, R., Schnipenkoetter, W., Hayden, M. 2010. An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor. Appl. Genet. 121:1083–1091.
Kadkhodaei, M., Dadkhodaie, A., TaghiAssad, M., Heidari, B., Mostowfizadeh-Ghalamfarsa, R. 2012. Identification of the leaf rust resistance genes Lr9, Lr26, Lr28, Lr34, and Lr35 in a collection of Iranian wheat genotypes using STS and SCAR markers. J. Crop Sci. Biotech. 15:267–274.
Karelov, A.V., Pirko, Ya.V., Kozub, N.A., Sozinov, I.A., Pirko, N.N., Litvinenko, N.A., Lyfenko, S.F., Koliuchii, V.T., Sozinov, A.A. 2011. Identification of the allelic state of the Lr34 leaf rust resistance gene in soft winter wheat cultivars developed in Ukraine. Cytology and Genetics 45:271–276.
Kloppers, F.J., Pretorius, Z.A. 1997. Effects of combinations amongst genes Lr13, Lr34 and Lr37 on components of resistance in wheat to leaf rust. Plant Pathol. 46:737–750.
Kolmer, J.A., Singh, R.P., Garvin, D.F., Viccars, L., William, H.M., Huerta-Espino, J., Ogbonnaya, F.C., Raman, H., Orford, S., Bariana, H.S., Lagudah, E.S. 2008. Analysis of the Lr34/Yr28 rust resistance region in wheat germplasm. Crop Sci. 48:1841–1852.
Krattinger, S.G., Lagudah, E.S., Spielmeyer, W., Singh, R.P., Huerta-Espino, J., McFadden, H., Bossolini, E., Selter, L.L., Keller, B. 2009. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363.
Lagudah, E.S. 2011. Molecular genetics of race non-specific rust resistance in wheat. Euphytica 179:81–91.
Lagudah, E.S., Krattinger, S.G., Herrera-Foessel, S., Singh, R.P., Huerta-Espino, J., Spielmeyer, W., Brown-Guedira, G., Selter, L.L., Keller, B. 2009. Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor. Appl. Genet. 119:889–898.
Lagudah, E.S., McFadden, H., Singh, R.P., Huerta-Espino, J., Bariana, H.S., Spielmeyer, W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114:21–30.
Li, T., Bai, G.H., Gu, S.L. 2012. A combination of leaf rust resistance gene Lr34 and lesion mimic gene lm significantly enhances adult plant resistance to Puccinia triticina in wheat. Chin. Sci. Bull. 57:2113–2119.
Lillemo, M., Asalf, B., Singh, R.P., Huerta-Espino, J., Chen, X.M., He, X.H., Bjornstad, Å. 2008. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor. Appl. Genet. 116:1155–1166.
Lillemo, M., Joshi, A.K., Prasad, R., Chand, R., Singh, R.P. 2013. QTL for spot blotch resistance in bread wheat line Saar co-locate to the biotrophic disease resistance loci Lr34 and Lr46. Theor. Appl. Genet. 126:711–719.
McCallum, B.D., Humphreys, D.G., Somers, D.J., Dakouri, A., Cloutier, S. 2012. Allelic variation for the rust resistance gene Lr34/Yr18 in Canadian wheat cultivars. Euphytica 183:261–274.
Purnhauser, L., Fónad, P., Kótai, E., Csõsz, M. 2011. Disease resistance of Hungarian and Romanian wheat cultivars observed by field and laboratory methods. “Strong wheat” Proc. Closing Conference. Timisoara, Romania, pp. 11–16.
Singh, D., Mohler, V., Park, R.F. 2013. Discovery, characterisation and mapping of wheat leaf rust resistance gene Lr71. Euphytica 190:131–136.
Singh, R., Herrera-Foessel, S.A., Huerta-Espino, J., Bariana, H.S., Bansal, U., McCallum, B.D., Hiebert, C.W., Bhavani, S., Singh, S., Lan, C., Lagudah, E.S. 2012. Lr34/Yr18/Sr57/Pm38/Bdv1/Ltn1 confers slow rusting, adult plant resistance to Puccinia graminis tritici. In: Proc. 13th Cereal Rust and Powdery Mildew Conference. Beijing, China, p. 173.
Singh, R.P. 1992. Association between gene Lr34 for leaf rust and leaf tip necrosis in wheat. Crop Sci. 32:874–878.
Singh, R.P., Mujeeb-Kazi, A., Huerta-Espino, J. 1998. Lr46: A gene conferring slow-rusting resistance to leaf rust in wheat. Phytopathol. 88:890–894.
Singh, R.P., Huerta-Espino, J. 2003. Effect of leaf rust resistance gene Lr34 on components of slow rusting at seven growth stages in wheat. Euphytica 129:371–376.
Spielmeyer, W., McIntosh, R.A., Kolmer, J., Lagudah, E.S. 2005. Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor. Appl. Genet. 111:731–735.
Vanzetti, L.S., Campos, P., Demichelis, M., Lombardo, L.A., Aurelia, P.R., Vaschetto, L.M., Bainotti, C.T., Helguera, M. 2011. Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers. Electronic J. Biotechnology https://doi.org/www.ejbiotechnology.info DOI: https://doi.org/10.2225/vol14-issue3-fulltext-14.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J. Kolmer
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Ciuca, M., Cristina, D., Turcu, A.G. et al. Molecular Detection of the Adult Plant Leaf Rust Resistance Gene Lr34 in Romanian Winter Wheat Germplasm. CEREAL RESEARCH COMMUNICATIONS 43, 249–259 (2015). https://doi.org/10.1556/CRC.2014.0040
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/CRC.2014.0040