Abstract
The characterization of the old Hungarian varieties and landraces is an important part of Hungarian cereal research and breeding. Analysis of these germplasms with the most up-to-date methodologies results a broad scale of diversity of glutenin alleles, which proves their genetic heterogenicity. Exploitation of this attribute is an untapped possibility for developing modern varieties in our breeding programs. The previous research work revealed this diversity by SDS-PAGE analysis and MALDI-TOF technology. The powerful tool, the high throughput lab-on-a chip technique can facilitate the effectiveness of this function and decreases the cost of the analysis. This study demonstrates the application of this technique for analysing the old varieties. The allelic composition and their effects on bread making quality concluded by means of functional analysis.
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Abbreviations
- HMW GS:
-
high molecular weight glutenin subunits
- LMW GS:
-
low molecular weight glutenin subunits
- LOC:
-
lab-on-a-chip
- MALDI-TOF-MS:
-
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- SDS-PAGE:
-
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- SE-HPLC:
-
size-exclusion high performance liquid chromatography
- UPP:
-
unextractable polymeric protein
- BEU:
-
Brabender Extensograph Unit
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Kovács, A., Rakszegi, M., Láng, L. et al. Application of a Rapid Electrophoresis Technique Analysing the Glutenin Subunit Composition of Wheat Genotypes. CEREAL RESEARCH COMMUNICATIONS 41, 468–481 (2013). https://doi.org/10.1556/CRC.2013.0010
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DOI: https://doi.org/10.1556/CRC.2013.0010