Abstract
Modification in the metabolism of phenolic compounds under boron (B) deficiency conditions was studied in tea plants. Plants were grown from seed, treated with low B in hydroponic medium under environmentally controlled conditions for six weeks. Dry matter production and B content of plants were significantly declined under B deficiency conditions. Boron starvation resulted in rising phenylalanine ammonia lyase activity in the young leaves and declining polyphenol oxidase activity in the roots. Soluble phenolics fraction was increased up to 3.4-fold in the young leaves while did not influence by B nutrition in the old leaves and roots. Cell wall (CW) bound phenolics and lignin content was lower in B-deficient plants compared with B-sufficient ones. Boron deficiency increased significantly activity of soluble peroxidase (POD) only in the leaves. Activity of ionically bound POD was decreased in the old leaf and roots while it increased in the young leaves upon B deprivation. Activity of covalently bound POD decreased in the roots and leaves of different age in low B plants. Our results suggested that tea plant is highly tolerant species to B deficiency and CW tightening and accumulation of oxidized phenolics are not mechanisms for growth inhibition under B deficiency conditions.
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Asad, A., Bell, R. W., Dell, B., Huang, L. (1997) Development of a boron buffered solution culture system for controlled studies of plant boron nutrition. Plant Soil 188, 21–32.
Bacon, M. A., Thompson, D. S., Davies, W. J. (1997) Can cell wall peroxidase activity explain the leaf growth response of Lolium temulentum L. during drought? J. Exp. Bot. 48, 2075–2085.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Broadley, M., Brown, P., Cakmak, I., Rengel, Z., Zhao, F. (2011) Function of nutrients: Micronutrients. In: Marschner, P. (ed.) Marschner’s Mineral Nutrition of Higher Plants. Academic Press, UK, pp. 191–248.
Brown, P. H., Bellaloui, N., Wimmer, M. A., Bassil, E. S., Ruiz, J., Hu, H., Pfeffer, H., Dannel, F., Römheld, V. (2002) Boron in plant biology. Plant Biol. 4, 205–223.
Cakmak, I., Römheld, V. (1997) Boron deficiency-induced impairments of cellular functions in plants. Plant Soil 193, 71–83.
Camacho-Cristóbal, J. J., Anzelotti, D., González-Fontes, A. (2002) Changes in phenolic metabolism of tobacco plants during short-term boron deficiency. Plant Physiol. Biochem. 40, 997–1002.
Camacho-Cristóbal, J. J., Herrera-Rodríguez, M. B., Beato, V. M., Rexach, J., Navarro-Gochicoa, M. T., Maldonado, J. M., González-Fontes, A. (2008) The expression of several cell wall-related genes in Arabidopsis roots is down-regulated under boron deficiency. Environ. Exp. Bot. 63, 351–358.
Cara, F. A., Sánchez, E. J., Ruiz, M., Romero, L. (2002) Is phenol oxidation responsible for the shortterm effects of boron deficiency on plasma-membrane permeability and function in squash roots? Plant Physiol. Biochem. 40, 853–858.
Dickerson, D. P., Pascholati, S. F., Hagerman, A. E., Butler, L. G., Nicholson, R. L. (1984) Phenylalanine ammonia-lyase and hydroxyl cinnamate CoA ligase in maize mesocotyls inoculated with Helminthosporium maydis or Helminthosporium carbonum. Physiol. Plant Pathol. 25, 111–123.
Dixon, R. A., Paiva, N. L. (1995) Stress-induced phenylpropanoid metabolism. The Plant Cell 7, 1085–1097.
Ghanati, F., Morita, A., Yokota, H. (2002) Induction of suberin and increase of lignin content by excess boron in tobacco cells. Soil Sci. Plant Nutr. 48, 357–364.
Hajiboland, R., Bastani, B., Bahrami-Rad, S. (2011) Photosynthesis, nitrogen metabolism and antioxidant defense system in B-deficient tea (Camellia sinensis (L.) O. Kuntze) plants. J. Sci. I. R. Iran 22, 311–320.
Hajiboland, R., Bastani, B., Bahrami-Rad, S. (2011) Effect of light intensity on photosynthesis and antioxidant defense in boron deficient tea plants. Acta Biol. Szeged 55, 265–272.
Hajiboland, R., Farhanghi, F. (2010) Remobilization of boron, photosynthesis, phenolic metabolism and anti-oxidant defense capacity in boron-deficient turnip (Brassica rapa L.) plants. Soil Sci. Plant Nutr. 56, 427–437.
Herms, D. A., Mattson, W. J. (1992) The dilemma of plants: to growth or defend. Quart. Rev. Biol. 67, 283–335.
Juszczuk, I. M., Wiktorowska, A., Malusá, E., Rychter, A. M. (2004) Changes in the concentration of phenolic compounds and exudation induced by phosphate deficiency in bean plants (Phaseolus vulgaris L.). Plant Soil 267, 41–49.
Kovácik, J., Backor, M. (2007) Changes of phenolic metabolism and oxidative status in nitrogendeficient Matricaria chamomilla plants. Plant Soil 297, 255–265.
Lewis, N. G., Yamamoto, E. (1990) Lignin: occurrence, biogenesis and biodegradation. Annu. Rev. Physiol. Plant Mol. Biol. 41, 455–496.
Liakopoulos, G., Karabourniotis, G. (2005) Boron deficiency and concentrations and composition of phenolic compounds in Olea europaea leaves: a combined growth chamber and field study. Tree Physiol. 25, 307–315.
Lohse, G. (1982) Microanalytical azomethine-H method for boron determination in plant tissues. Commun. Soil Plant Anal. 13, 127–134.
Mahanta, P. K, Baruah, S. (1992) Changes in pigments and phenolics and their relationship with black tea quality. J. Sci. Food Agric. 59, 21–26.
Malusà, E., Russo, M. A, Mozzetti, C., Belligno, A. (2006) Modification of secondary metabolism and flavonoid biosynthesis under phosphate deficiency in bean roots. J. Plant Nutr. 29, 245–258.
Nagata, T., Hayatsu, M., Kosuge, N. (1992) Identification of aluminium forms in tealeaves by 27Al NMR. Phytochem. 31, 1215–1218.
Pillinger, J. M, Cooper, J. A, Ridge, I. (1994) Role of phenolic compounds in the antialgal activity of barley straw. J. Chem. Ecol. 20, 1557–1569.
Ranieri, A., Castagna, A., Baldan, B., Soldatini, G. F (2001) Iron deficiency differently affects peroxidase isoforms in sunflower. J. Exp. Bot. 52, 25–35.
Ruiz, J. M, Bretones, G., Baghour, M., Ragala, L., Belakbir, A., Romero, L. (1998) Relationship between boron and phenolic metabolism in tobacco leaves. Phytochem. 48, 269–272.
Salehi, S. Y, Hajiboland, R. (2008) A high internal phosphorus use efficiency in tea (Camellia sinensis L.) plants. Asian J. Plant Sci. 7, 30–36.
Shorrocks, V. M (1997) The occurrence and correction of boron deficiency. Plant Soil 193, 121–148.
Siegel, B. Z (1993) Plant peroxidases-an organismic perspective. Plant Growth Regul. 12, 303–312.
Singh, N., Singh, R., Kaur, K., Singh, H. (1999) Studies of the physico-chemical properties and polyphenoloxidase activity in seeds from hybrid sunflower (Helianthus annuus) varieties grown in India. Food. Chem. 66, 241–247.
Solecka, D. (1997) Role of phenylpropanoid compounds in plant responses to different stress factors. Acta Physiol. Plant. 19, 257–268.
Solecka, D., Boudet, A.-M., Kacperska, A. (1999) Phenylpropanoid and anthocyanin changes in lowtemperature treated winter oilseed rape leaves. Plant Physiol. Biochem. 37, 491–496.
Swain, T., Hillis, E. E (1959) The phenolic constituents of Prunus domestica I. The quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 63–68.
Van Huystee, R. B, Zheng, X. (1995) Peanut peroxidase, its location and extension, coniferyl oxidation. Plant Physiol. Biochem. 33, 55–60..
Wu, Y., Cosgrove, D. J (2000) Adaptation of roots to low water potentials by changes in cell wall extensibility and cell wall proteins. J. Exp. Bot. 51, 1543–1553.
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Hajiboland, R., Bahrami-Rad, S. & Bastani, S. Phenolics Metabolism in Boron-Deficient Tea [Camellia Sinensis (L.) O. Kuntze] Plants. BIOLOGIA FUTURA 64, 196–206 (2013). https://doi.org/10.1556/ABiol.64.2013.2.6
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DOI: https://doi.org/10.1556/ABiol.64.2013.2.6