Abstract
Accumulation of oxidative damage caused by reactive oxygen species (ROS) underlies fundamental changes found during aging. In the present study, age related effect on testicular mitochondrial oxidant generation and antioxidant defence profile was investigated in Wistar rats at 3 months (young adults), 12 months (old adults) and 24 months (senescent animals) of age. Mitochondrial oxidative stress parameters viz., lipid peroxidation (LPx), protein carbonylation, hydrogen peroxide (H202) generation and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), levels of total, oxidized (GSSG) and reduced glutathione (GSH) were studied to find out their roles in maintenance of mitochondrial glutathione redox pool as a function of age. Increased levels of LPx, H202 and decreased GSH content accompanied by a decline in activities of SOD, GPx and GR with advancing age suggest that antioxidant defense profile of testicular mitochondria exhibit age related alterations which might play a critical role in regulating physiological functions of the testis such as steroidogenesis and spermatogenesis.
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Cao, L., Leers-Sucheta, S., Azhar, S. (2004) Aging alters the functional expression of enzymatic and non-enzymatic antioxidant defence system in testicular rat Leydig cells. J. Steroid Biochem. Mol. Biol. 88, 61–70.
Capel, F., Rimbert, V., Lioger, D., Diot, A., Rousset, P., Mirand, P. P., Boirie, Y., Morio, B., Mosoni, L. (2005) Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech. Ageing Dev. 126, 505–511.
Carmagnol, F., Sinet, P. M., Jerome, H. (1983) Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts. Biochim. Biophys. Acta 759, 49–57.
Chen, J., Irizarry, R. A., Luo, L., Zirkin, B. R. (2004) Leydig cells gene expression: Effect of age and caloric restriction. Expt. Gerontol. 39, 31–40.
Cohen, G., Dembiec, D., Marcus, J. (1970) Measurement of catalase activity in tissue extracts. Anal. Biochem. 34, 30–38.
Das, K., Samanta, L., Chainy, G. B. N. (2000) A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals. Ind. J. Biochem. Biophys. 37, 201–204.
de la Asuncion, J. G., Millan, A., Pla, R., Bruseghini, L., Esteras, A., Pallardo, F. V., Sastre, J., Vina, J. (1996) Mitochondrial glutathione oxidation correlates with age-associated oxidative damage to mitochondrial DNA. FASEB J. 10, 333–338.
Fucci, L., Oliver, C. N., Coon, M. J., Stadtman, E. R. (1983) Inactivation of key metabolic enzymes by mixed function oxidation reactions: possible implication in protein turnover and aging. Proc. Nat. Acad. Sci. USA 80, 1521–1525.
Harman, D. (1956) Aging: a theory based on free radical and radiation chemistry. J. Gerontol. 11, 298–300.
Kaur, A. (2005) Changes in peroxidase activity and structure of testes, epididymis and seminal vesicles in aging Swiss albino mice. Ind. J. Gerontol. 19, 119–126.
Kumaran, S., Deepak, B., Naveen, B., Panneerselvam, C. (2003) Effects of levocarnitine on mitochondrial antioxidant systems and oxidative stress in aged rats. Drugs R.D. 4, 141–147.
Lee, C. Y. (1982) Glucose-6-phosphate dehydrogenase from mouse. Methods Enzymol. 89, 252–260.
Lee, H. C., Wei, Y. H. (2000) Mitochondrial role in life and death of the cell. J. Biomed. Sci. 7, 2–15.
Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A. G., Ahn, B. W., Shaltiel, S., Stadtman, E. R. (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 186, 464–478.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 193, 265–275.
Luo, L., Chen, H., Trush, M. A., Show, M. D., Anway, M. D., Zirkin, B. R. (2006) Aging and the brown Norway rat Leydig cell antioxidant defence system. J. Androl. 27, 240–247.
Massey, V., Williams, C. H. (1965) On the reaction mechanism of yeast glutathione reductase. J. Biol. Chem. 240, 4470–4481.
Matsubara, L. S., Machado, P. E. A. (1991) Age related changes of glutathione content, glutathione reductase and glutathione peroxidase activity of human erythrocytes. Brazil. J. Med. Biol. Res. 24, 449–459.
Miquel, J., Economos, A. C, Fleming, J., Johnson, J. E. Jr. (1980) Mitochondrial role in cell aging. Exp. Gerontol. 15, 579–591.
Nagley, P., Wei, Y. H. (1998) Ageing and mammalian mitochondrial genetics. Trends Genet. 14, 513–517.
Navarro, A., Boveris, A. (2004) Rat brain and liver mitochondria develop oxidative stress and lose enzymatic activities on aging. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287, R1244–R1249.
Ohkawa, H., Ohishi, N., Yagi, K. (1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95, 351–358.
Paglia, D. E., Valentine, W. N. (1967) Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70, 158–169.
Peltola, V., Huhtaniemi, I., Ahotupa, M. (1992) Antioxidant enzyme activity in the maturing rat testis. J. Androl. 13, 450–455.
Pick, E., Keisari, Y. (1981) Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli. Cell. Immunol. 59, 301–318.
Sahoo, D. K., Roy, A., Bhanja, S., Chainy, G. B. N. (2005) Experimental hyperthyroidism-induced oxidative stress and impairment of antioxidant defence system in rat testis. Ind. J. Exp. Biol. 43, 1058–1067.
Sahoo, D. K., Roy, A., Bhanja, S., Chainy, G. B. N. (2008) Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation. Gen. Comp. Endocrinol. 156, 63–70.
Sahoo, D. K., Roy, A., Chattopadhyay, S., Chainy, G. B. N. (2007) Effect of T3 treatment on glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes. Ind. J. Exp. Biol. 45, 338–346.
Samanta, L., Chainy, G. B. N. (1997) Comparison of hexachlorocyclohexane-induced oxidative stress in the testis of immature and adult rats. Comp. Biochem. Physiol. 118, 319–327.
Samanta, L., Chainy, G. B. N. (2002) Effect of age on testicular antioxidant defence system. Ind. J. Gerontol. 16, 69–78.
Samanta, L., Roy, A., Chainy, G. B. N. (1999) Changes in rat testicular antioxidant defence profile as a function of age and its impairment by hexachlorocyclohexane during critical stages of maturation. Andrologia 31, 83–90.
Sastre, J., Pallardo, F. V., Vina, J. (2003) The role of mitochondrial oxidative stress in aging. Free Rad. Biol. Med. 35, 1–8.
Sharma, R., Nakamura, A., Takahashi, R., Nakamoto, H., Goto, S. (2006) Carbonyl modification in rat liver histones: Decrease with age and increase by dietary restriction. Free Rad. Biol. Med. 40, 1179–1184.
Shigenaga, M. K., Hagen, T. M., Ames, B. N. (1994) Oxidative damage and mitochondrial decay in aging. Proc. Natl. Acad. Sci. USA 91, 10771–10778.
Sohal, R. S. (1993) The free radical hypothesis of aging: an appraisal of the current status. Aging Clin. Exp. Res. 5, 3–17.
Sohal, R. S., Arnold, L. A., Sohal, B. H. (1990) Age related changes in antioxidant enzymes and prooxidant generation in tissues of the rat with special reference to parameters in two insect species. Free Radic. Biol. Med. 10, 495–500.
Teaf, C. M., Harbison, R. D., Bishop, J. B. (1985) Germ-cell mutagenesis and GSH depression in reproductive tissue of the F-344 rat induced by ethyl methanesulfonate. Mut. Res. 144, 93–98.
Zirkin, B. R., Chen, H. (2000) Regulation of leydig cell steroidogenic function during aging. Biol. Reprod. 63, 977–981.
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Sahoo, D.K., Roy, A. & Chainy, G.B.N. Rat Testicular Mitochondrial Antioxidant Defence System and its Modulation By Aging. BIOLOGIA FUTURA 59, 413–424 (2008). https://doi.org/10.1556/ABiol.59.2008.4.3
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DOI: https://doi.org/10.1556/ABiol.59.2008.4.3