Abstract
Leaf rust, caused by Puccinia recondita f. sp. tritici, is one of the major diseases of wheat in Kazakhstan. To effectively use leaf rust resistance genes (Lr), it is important for breeders to know the resistance genotype in current cultivars. In this study, 30 winter wheat entries grown and/or produced in Kazakhstan were investigated using molecular markers to determine the presence and absence of eight important Lr genes. Molecular screening of these genotypes showed contrasting differences in the frequencies of these genes. Among the 30 entries, 17 carried leaf rust resistance gene Lr1, six had Lr26 and Lr34, and Lr10 and Lr37 were found in three cultivars. Two single cultivars separately carried Lr19 and Lr68, while Lr9 was not detected in any genotypes in this study. Field evaluation demonstrated that two of the most frequent two genes (Lr1 and Lr26) to be ineffective. While Lr34 provided some protection, the remaining effective Lr genes were found only in few genotypes: Lr37 occurred in Kazakh genotypes L-1090 and Krasnovodapadskaya 210 and in the US cultivar Madsen; Lr19 and Lr68 were likely present only in Russian and Kazakh cultivars, Pallada and Yegemen, respectively. The highest resistance over three years of leaf rust testing was found in Kazakh cultivars, Karasay, Krasnovodapadskaya 210, L-1090, Arap and Yegmen, foreign cultivars Madsen, Pallada and the control Parula (Lr68). Data may assist breeders to incorporate effective Lr genes into new cultivars.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bartoš, P., Ovesna, J., Hanzalova, A., Chrpova, J., Dumalasova, V., Skorpik, M., Sip, V. 2004. Presence of a translocation from Aegilops ventricosa in wheat cultivars registered in the Czech Republic. Czech J. Genet. Plant Breed. 40:31–35.
Feuillet, C., Messmer, M., Schachermayr, G., Keller, B. 1995. Genetic and physical characterization of the Lr1 leaf rust resistance locus in wheat (Triticum aestivum L.). Mol. Gen. Genet. 248:553–562.
Gultyaeva, E.I. 2012. Methods for the identification of genes for resistance of wheat to leaf rust using DNA markers and characteristics of effective Lr genes. Russian Academy of Agricultural Sciences: VIZR. Saint-Petersburg, Russia. 72 p.
Helguera, M., Khan, I.A., Kolmer, J., Lijavetzky, D., Zhong-qi, L., Dubcovsky, J. 2003. PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Sci. 43:1839–1847.
Herrera-Foessel, S.A., Singh, R.P., Huerta-Espino, J., Rosewarne, G.M., Periyannan, S.K., Viccar, L., Calvo-Salazar, V., Lan, C., Lagudah, E.S. 2012. Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor. Appl. Genet. 124:1475–1486.
Koishybayev, M.K., Zhanarbekova, A.B., Kokhmetova, A.V., Rsaliev, Sh.S. 2010. Genetic study of wheat resistance to leaf rust. Bulletin of National Academy of Sciences of Kazakhstan 6:10–15.
Kokhmetova, A., Sapakhova, Z., Madenova, A., Yessenbekova, G. 2014. Identification of carriers of yellow Yr5, Yr10, Yr15 and leaf Lr26, Lr34 rust resistance genes under molecular screening of wheat entries. Biotechnology. Theory and Practice 1:71–78.
Kosman, E., Pardes, E., Anikster, Y., Manisterski, J., Yehuda, P.B., Szabo, L.J., Sharon, A. 2004. Genetic variation and virulence on Lr26 in Puccinia triticina. Phytopathol. 94:632–640.
Lagudah, E.S., McFadden, H., Singh, R.P., Huerta-Espino, J., Bariana, H.S., Spielmeyer, W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114:21–30.
Lillemo, M., Singh, R.P., William, M., Herrera-Foessel, S.A., Huerta Espino, J., German, S., Campos, P., Chaves, M., Madriaga, R., Xia, X.C., Liang, S.S., Liu, D., Li, Z.F., Lagudah, E.S. 2011. Multiple rust resistance and gene additivity in wheat: Lessons from multi-location case studies in the cultivars Parula and Saar. BGRI Technical Workshop. St. Paul, Minnesota, USA. pp. 111–120.
McIntosh, R.A., Wellings, C.R., Park, R.F. 1995. Wheat Rusts: An Atlas of Resistance Genes. CSIRO. Sydney, Australia. pp. 234–237.
McIntosh, R.A., Dubcovsky, J., Rogers, J., Morris, C., Appels, R., Xia, X. 2010. Catalogue of gene symbols for wheat: 2010 supplement. http: www.shigen.nig.ac.jp/wheat/komugi/genes/macgene 2010
Moreno-Sevilla, B., Baenziger, P.S., Peterson C.J., Graybosch, R.A., McVey, D.V. 1995. The 1BL/1RS translocation: Agronomic performance of F3-derived lines from a winter wheat cross. Crop Sci. 35:1051–1055.
Pathan, A.K., Park, R.F. 2006. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica 149:327–342.
Peterson, R.F., Campbell, A.B., Hannah, A.E. 1948. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can. J. Res. Sect. C 26:496–500.
Pretorius, Z.A. 1988. First report of virulence to wheat leaf rust resistance gene Lr26 in South Africa. Plant Dis. 72:175.
Prins, R., Groenewald, J.Z., Marais, G.F., Snape, J.W., Koebner, R.M.D. 2001. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 103:618–624.
Riede, C.R., Anderson, J.A. 1996. Linkage of RFLP markers to an aluminum tolerance gene in wheat. Crop Sci. 36:905–909.
Roelfs, A.P., Singh, R.P., Saari, E.E. 1992. Rust diseases of wheat: Concepts and methods of disease management. CIMMYT. Mexico, D.F., Mexico. 81 p.
Rsaliev, A.S. 2009. Resistance of spring durum wheat varieties (Triticum durum Desf) to rust diseases. Ph.D thesis of a dissertation for the Degree of Candidate of Agricultural Sciences on specialty 06.01.05 Plant Breeding and Seed Growing. CopyLand. Almalybak, Kazakhstan. 25 p.
Rsaliev, Sh.S., Sedlovskii, A.I., Rsaliev, A.S., Tileubaeva, Zh.S., Tyupina, L.N., Yessenbekova, G.T., Atishova, M.N., Agabayeva, A.Ch. 2011. Catalogue of wheat cultivars and samples with resistance genes to leaf rust. IP Volkova. Almaty, Kazakhstan. 100 p.
Schachermayr, G., Siedler, H., Gale, M.D., Winzeler, H., Winzeler, M., Keller, B. 1994. Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theor. Appl. Genet. 88:110–115.
Schachermayr, G., Feuillet, C., Keller, B. 1997. Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds. Mol. Breeding 3:65–74.
Singh, R.P., Huerta-Espino, J., Rajaram, S., Crossa, J. 1998. Agronomic effects from chromosome translocations 7DL.7 Ag and 1BL.1RS in spring wheat. Crop Sci. 38:27–33.
Stubbs, R.W., Prescott, E.E., Saari, E.E., Dubin, H.J. 1986. Cereal disease methodology manual. CIMMYT. Mexico D.F., Mexico. 46 p.
Viuchkov, A.A. 1998. Breeding of spring bread and durum wheat in Middle Povoloje. Thesis of dissertation for the degree of Doctor Agricultural Sciences: 06.01.05. Bezenchuk, Russia. 66 p.
Weng, Y., Azhaguvel, P., Devkota, R.N., Rudd, J.C. 2007. PCR-based markers for detection of different sources of 1AL.1RS and 1BL.1RS wheat-rye translocation in wheat background. Plant Breed. 126:482–486.
Acknowledgements
The authors acknowledge to researchers of the Laboratory of Genetics and Breeding of the Institute of Plant Biology and Biotechnology, Department of the Gene Pool of the Kazakh Research Institute of Agriculture and Plant Growing for contributions in accessing the field data and in promoting research. Special thanks to Dr. J. A. Kolmer (USDA-ARS, St Paul, MN, USA) for providing us the Lr-NILs of cv. Thatcher. The authors gratefully thank to Dr. Colin Wellings (University of Sydney) for the accurate correction of manusscript.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J. Kolmer
Rights and permissions
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Kokhmetova, A., Madenova, A., Kampitova, G. et al. Identification of Leaf Rust Resistance Genes in Wheat Cultivars Produced in Kazakhstan. CEREAL RESEARCH COMMUNICATIONS 44, 240–250 (2016). https://doi.org/10.1556/0806.43.2015.056
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1556/0806.43.2015.056