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PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania

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Abstract

The members of the Anopheles maculipennis complex have been incriminated for the transmission of the malaria in Europe, which was endemic until the middle of the century. The global warming and the intensification of the intercontinental travel constitute a risk of the re-emergence of the malaria in Europe, given the presence of the Anopheles vectors. The study has attempted the identification by using the PCR (Polymerase Chain Reaction) of the members of the Anopheles maculipennis complex from the North-eastern area of Romania from the city of Iaşi. In total there have been identified by using the PCR amplifying the ITS2 sequence of the ribosomal DNA, 217 specimens belonging to the complex of A. maculipennis among which: 58 A. atroparvus, 18 A. melanoon, 2 A. labranchiae, 52 A. maculipennis and 87 A. messeae. The ITS2 sequences of the ribosomal DNA have been compared to those of the species belonging to the A. maculipennis available in GenBank. The Species A. labranchiae is reported for the first time in Romania, being identified in the larval stage IV. The adaptation of a new species to the climatic conditions present in the North-eastern Romania, confirms the phenomenon of global warming and also the intensification of the travelling. As a result of the analysis of the A. labranchiae sequence, this one corresponds to the extent of 96% to the species from Italy, registered in GenBank, given the fact that a high number of the inhabitants of the municipality of Iaşi are working in this country.

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References

  • Alten B., Kampen H., Fontenille D. 2007. Malaria in Southern Europe: resurgence from the past?. In: (Eds. W. Takken, B. G.Knols), Emerging Pests and Vector-Borne Diseases in Europe Vol.1. Wageningen University Press, 35–57. DOI: 10.3920/978-90-8686-626-7

    Google Scholar 

  • Becker N., Petric D., Boase C., Zgomba M., Dahl C., Lane J., Kaiser A. 2003. Mosquitoes and their Control. Kluwer Academic/Plenum Publishers, New York. 163–186

    Google Scholar 

  • Bietolini S., Candura F., Coluzzi M. 2006. Spatial and long term temporal distribution of the Anopheles maculipennis complex species in Italy. Parassitologia, 48, 581–608. Cote INIST: 26177, 35400014676523.0060

    CAS  PubMed  Google Scholar 

  • Calleri G., Behrens R.H., Schmid M.L., Gobbi F., Grobusch M.P., Castelli F., Gascon J., Bisoffi Z., Jelinek T., Caramello P. 2011. Malaria chemoprophylaxis recommendations for immigrants to Europe, visiting relatives and friends - a Delphi method study. Malaria Journal, 10, 137. DOI: http://dx.doi.org/10.1186/1475-2875-10-137

    Article  Google Scholar 

  • Cobo F. 2014. Current status of malaria, Imported Infectious Diseases, The Impact in Developed Countries, 1st Edition, Elsevier, 61–90

    Book  Google Scholar 

  • Di Luca M., Boccolini D., Severini F., Toma L., Mancini-Barbieri F., Massa A., Romi R. 2009. A 2-Year Entomological Study of Potential Malaria Vectors in Central Italy. Vector-Borne and Zoonotic Diseases, 9, 703–711. DOI: 10.1089/vbz.2008.0129

    Article  Google Scholar 

  • Djadid N.D., Gholizadeh S., Tafsiri E., Romi R., Gordeev M., Zakeri S., 2007. Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran. Malar Journal, 6, 6. DOI: 10.1186/1475-2875-6-6

    Article  Google Scholar 

  • Kronefeld M., Dittmann M., Zielke D., Werner D., Kampen H., 2012. Molecular confirmation of the occurrence in Germany of Anopheles daciae (Diptera, Culicidae). Parasites & Vectors, 5, 250. DOI: 10.1186/1756-3305-5-250

    Article  CAS  Google Scholar 

  • Mouchet J., Carnevale P., Coosemans M., Julvez J., Manguin S., Richard-Lenoble D., Sircoulon J., 2004. Biodiversité du paludisme dans le monde, Paris: John Libbey Eurotext, 425 pp.

    Google Scholar 

  • Nitzulescu V. and Gherman I., 1990. Entomologie medicală, Editura Academiei Romane, 117–129

    Google Scholar 

  • Nicolescu G. and Purcărea-Ciulacu V. 2012. Modelul de stratificare a zonelor de risc de re-emergenţă şi al strategiei de supraveghere ş i control al malariei în România. Edit. Ars Docendi-Universitatea din Bucuresti

    Google Scholar 

  • Nicolescu G., Linton Y.M., Vladimirescu A., Howard T.M., Harbach R.E. 2004. Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence. Bulletin of Entomological Research, 94, 525–35. DOI: http://dx.doi.org/10.1079/BER2004330

    Article  CAS  Google Scholar 

  • Odolini S., Gautret P., Parola P. 2012. Epidemiology of Imported Malaria in the Mediterranean Region. Mediterranean Journal of Hematology and Infections Diseases, 4, e2012031. DOI: 10.4084/MJHID.2012.031

    Article  Google Scholar 

  • Proft J., Maier A.M., Kampen H., 1999. Identifcation of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay. Parasitol Research (1999) 85, 837–843. DOI: 10.1007/s004360050642

    Article  CAS  Google Scholar 

  • Pages F., Orlandi-Pradines E., Corbel V. 2007. Vecteurs du paludisme:biologie, diversite, controle et protection individuelle. Médecine et maladies infectieuses 37, 153–161. DOI: 10.1016/j.medmal.2006.10.009

    Article  CAS  Google Scholar 

  • Patz A.J., Olson H.S. 2006. Malaria risk and temperature: Influences from global climate change and local land use practices. National Academy of Sciences of the United States of America, PNAS, vol. 103 no. 15. 5635–5636. DOI: 10.1073/pnas.0601493103

    Article  CAS  Google Scholar 

  • Romi R., Boccolini D., Menegon M., Rezza G. 2012. Probable autochthonous introduced malaria cases in Italy in 2009–2011 and the risk of local vector-borne transmission. Eurosurveillance. Volume 17, Issue 48, 29 November 17, 20325. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId= 20325

    Google Scholar 

  • Romi R., Sabatinelli G., Majori G. 2001. Could malaria reappear in Italy? Emerging Infections Diseases. Nov-Dec, 7, 915–919. DOI: 10.3201/eid0706.010601

    Article  CAS  Google Scholar 

  • Romi R., Boccolini D., D’Amato S., Corrado C., Peragallo M., D’Ancona F., Pompa M.G., Majori G. 2010. Incidence of malaria and risk factors in Italian travelers to malaria endemic countries. Travel Medicine and Infectious Disease, May, 8, 144–154. DOI: http://dx.doi.org/10.1016/j.tmaid. 2010.02.001

    Article  Google Scholar 

  • Shkurti K., Vyshka G., Velo E., Bocari A., Kokici M., Kraja D., 2013. Imported malaria in Albania and the risk factors that could allow its reappearance. Malar Journal. 2013, 12, 197. DOI: 10.1186/1475-2875-12-197

    Article  Google Scholar 

  • Sedaghat M.M., Linton Y.M., Oshaghi M.A., Vatandoost H., Harbach R.E. 2003. The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species. Bulletin of Entomological Research. Dec, 93, 527–535. DOI: http//dx.doi.org/10.1079/BER2003272

    Article  CAS  Google Scholar 

  • Sinka M. E., Bangs M.J., Manguin S., Coetzee M., Mbogo C.M., Hemingway J., Patil A.P., Temperley W.H., Gething P.W., Kabaria C.W., Okara R.M., Van Boeckel T., Godfray C.J., Harbach R.E., Hay S.I. 2010. The dominant Anopheles vectors of human malaria in Africa, Europe and the Middle East: occurrence data, distribution maps and bionomic precis. Parasites & Vectors, 3, 117. DOI: 10.1186/1756-3305-3-117

    Article  Google Scholar 

  • Sinka M. E. et al. 2012. A global map of dominant malaria vectors Parasites & Vectors, 5, 69. DOI: 10.1186/1756-3305-5-69

    Article  Google Scholar 

  • Tolle M. A. 2009. Mosquito-borne Diseases. in Current Problem in Pediatric and Adolescent Health Care, 39, 97–140. DOI: 10.1016/j.cppeds.2009.01.001

    Article  Google Scholar 

  • Vicente L., Sousa C. A., Alten B., Caglar S.S., Falcuta E., Latorre J.M., Toty C., Barre H., Demirci B., Di Luca M., Toma L., Alves R., SalgueiroP., SilvaT. L., BarguesM. D., Mas-ComaS., Boccolini D., Romi R., Nicolescu G., RosarioV. E., Ozer N., Fontenille D., Pinto J. 2011. Genetic and phenotypic variation of the malaria vector Anopheles atroparvus in southern Europe. Malaria Journal, 10, 5. DOI: 10.1186/1475-2875-10-5

    Article  Google Scholar 

  • Zahar A.R., 1990. Vector bionomics in the epidemiology and control of malaria. Part II. The WHO Europeanregion and the WHO Eastern Mediterranean region. Volume II: Applied field studies. Section III: Vector bionomics, malaria epidemiology and control by geographical areas. (A): The Mediterranean Basin. WHO; 226 pp.

    Google Scholar 

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Authors and Affiliations

  1. Department of Clinics, Faculty of Veterinary Medicine, “Ion Ionescu de la Brad”, University of Agricultural Sciences and Veterinary Medicine, 3, M. Sadoveanu, 700490, Iasi, Romania

    Maria-Larisa Ivanescu, Dumitru Acatrinei, Ionuţ Pavel & Liviu Miron

  2. Institute of Zoology, Academy of Sciences of Moldova, 1 Academy Street, MD-2028, Chişinău, Moldova

    Tatiana Sulesco

Authors
  1. Maria-Larisa Ivanescu
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  2. Dumitru Acatrinei
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  3. Ionuţ Pavel
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  4. Tatiana Sulesco
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  5. Liviu Miron
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Correspondence to Maria-Larisa Ivanescu.

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Ivanescu, ML., Acatrinei, D., Pavel, I. et al. PCR identification of five species from the Anopheles maculipennis complex (Diptera: Culicidae) in North-Eastern Romania. Acta Parasit. 60, 283–289 (2015). https://doi.org/10.1515/ap-2015-0040

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  • DOI: https://doi.org/10.1515/ap-2015-0040

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