Skip to main content

Advertisement

SpringerLink
Log in

The current state of steppe perennial plants populations: A case study on Iris pumila

  • Published:
Biologia Aims and scope Submit manuscript

Abstract

A comprehensive study of a typical steppe perennial Iris pumila L. were carried out in the central zone of the European part of this species’ range, namely in Ukraine. Intraspecific differentiation, population size and isolation degree and its consequences, the threat of human impact were analyzed, as well as ecological amplitude and genetic variation in ISSR markers and selected chloroplast regions were determined. The species was found to have a low intraspecific differentiation that indicates the uniformity of the gene pool in the studied part of the range. Moreover, the results of isolation assessment, population and ecological study of I. pumila confirm the potential risk of extinction. A considerable part of the species populations exist as separated patches of rare ecosystems, isolated from the nearest neighbours due to intense plowing of the steppe zone. The generative reproduction is rare. In contrast, ISSR analysis revealed comparably high genetic diversity in all the sampled populations. Furthermore, specific plastid haplotypes were demonstrated in some of them. The inconsistency between the results of population ecological study and the data of molecular genetic analysis indicates that the loss of genetic diversity in the species caused by habitat fragmentation and isolation under increasing anthropogenic pressure is likely to be slower than it would appear judging from the assessment of population parameters, which clearly show negative trends. This result also emphasizes the necessity for integrated approach to assessment of the extinction risk for particular species and careful analysis of all determinants of extinction.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AMOVA:

analysis of molecular variance

CTAB:

cetyltrimethylammonium bromide

DMSO:

dimethyl sulfoxide

ISSR:

Inter-Simple Sequence Repeat

obl.:

oblast

r-n:

raion

vil.:

village

References

  • Aguilar R., Quesada M., Ashworth L., Herreriasdiego Y. & Lobo J. 2008. Genetic consequences of habitat fragmentation in plant populations: susceptible signals in plant traits and methodological approaches. Mol. Ecol. 17: 5177–5188.

    Article  PubMed  Google Scholar 

  • Arafeh R.M.H., Sapir Y., Shmida A., Iraki N., Fragman O. & Comes H.P. 2002. Patterns of genetic and phenotypic variation in Iris haynei and I. atrofusca (Iris sect. Oncocyclus = the royal irises) along an ecogeographical gradient in Israel and the West Bank. Mol. Ecol. 11: 39–53.

    Article  CAS  PubMed  Google Scholar 

  • Artyukova E.V., Kozyrenko M.M., Ilyushko M.V., Zhuravlev Yu.N. & Reunova G.D. 2001. Genetic Variability of Iris setosa. Mol. Biol. (Mosk). 35: 134–138.

    Article  CAS  Google Scholar 

  • Bairak O.M. & Stetsiuk N.O. 2005. Atlas ridkisnkh ta znykayuchkh rosln Poltavshchini. Verstka, Poltava, 248 pp.

    Google Scholar 

  • Bairak O.M., Shevel I.M., Hrytsai I.A., Kryvoruchko T.V. & Nechytailo V.I. 2006. Botanichnii zakaznik Drabinivka. Verstka, Poltava, 81 pp.

    Google Scholar 

  • Bezusko L.G., Mosiakin S.L., Bezusko A.G. 2011. Zakonomirnosti ta tendentsiyi rozvytku roslynnoho pokryvu Ukrayiny u pizn’omu pleistotseni ta holotseni. Alterpress, Kyiv, 488 pp.

    Google Scholar 

  • Biodiversity Support Program. 1999. Priority setting in conservation: A new approach for Crimea. Washington, D.C., 257 pp.

    Google Scholar 

  • Bokov V.A. 1999. Perspektivi sozdaniya Edinoi prirodookhrannoi seti Kryma. Crimean educational pedagogical publishing house, Simferopol, 192 pp.

    Google Scholar 

  • Brown A., Young A., Burdon J., Christidis L., Clarke G., Coates D. & Sherwin W. 1997. Genetic indicators for state of the environment reporting, Australia: State of the Environment Technical Paper Series (Environmental Indicators), Department of the Environment, Sport and Territories, Canberra, 30 pp.

    Google Scholar 

  • Chopyk V.I., Bortniak M.M., Voitiuk Yu.O. et al. 1998. Konspekt flory Seredn’oho Prydniprov”ya. Fitosotsiotsentr, Kyiv, 139 pp.

    Google Scholar 

  • Dalmady J. 1972. Iris species on Slovenski Kras area. Nature Prot. 3: 64–65.

    Google Scholar 

  • Osoblivosti ontohenezu Iris pumila, pp. 246–247. In: Materialy naukovoyi konferentsiyi “Bilolohiya: vid molekuly do biosfery”, November 17–21, 2009, Kharkiv.

    Google Scholar 

  • Didenko I.P., Shvets T.A. & Tomashevska N.P. 2010. Ekolohotsenotychni osoblivosti Iris pumila L. v Kirovohrads’ki oblasti, pp. 206. In: Aktual’ni problemy botaniky ta ekolohiyi. Materialy mizhnarodnoyi konferentsiyi molodykh uchenykh, September 21–25, 2010, Yalta, Simpheropol.

    Google Scholar 

  • Didukh Ya.P. 2011. The ecological scales for the species of Ukrainian flora and their use in synphytoindication. Phytosociocentr, Kyiv, 176 pp.

    Google Scholar 

  • Didukh Ya.P. 2009. Zelena kniha Ukrainy. Altpress, Kyiv, 448 pp.

    Google Scholar 

  • Didukh Ya.P. 2009. Chervona kniha Ukrayiny. Roslynni svit. Globalconsulting, Kyiv, 900 pp.

    Google Scholar 

  • Dobrochaeva D.N., Kotov M.I., Prokudin Yu.N., et al. 1999. Opredelitel’ vysshikh rastenii Ukrainy. Fitosoctsiotsentr, Kiev, 548 pp.

    Google Scholar 

  • Doyle J.J. & Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.

    Google Scholar 

  • Ewers R.M. & Didham R.K. 2006. Confounding factors in the detection of species responses to habitat fragmentation. Biol. Rev. 81: 117–142.

    Article  PubMed  Google Scholar 

  • Fisher M., Musi R., Prater D., Peintinger M., Van Kleunen M., Shmidt B. 2000. RAPD variation among and within small and large populations of the rare clonal plant Ranunculus reptans (Ranunculaceae). Am. J. Bot. 87: 1128–1137.

    Article  Google Scholar 

  • Fedyaeva V.V., Shmaraeva A.N. & Shyshlova Zh.N. 2011. Monitoring populyatsii Iris pumila L. v Rostovskoi oblasti. Nauchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. Estestvennie nauki. 15: 326–331.

    Google Scholar 

  • Gouy M., Guindon S. & Gascuel O. 2010. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol. Biol. Evol. 27: 221–224.

    Article  CAS  PubMed  Google Scholar 

  • Kostrakiewicz K. & Wróblewska A. 2008. Low genetic variations in subpopulations of an endangered clonal plant Iris sibirica in Southern Poland. Ann. Bot. Fenn. 45: 186–194.

    Article  Google Scholar 

  • Kozyrenko M.M., Artyukova E.V. & Zhuravlev Yu.N. 2009. Independent species status of Iris vorobievii N.S. Pavlova, Iris mandshurica Maxim., and Iris humilis Georgi (Iridaceae): Evidence from the nuclear and chloroplast genomes. Russ. J. Genet. 45: 1394–1402.

    Article  CAS  Google Scholar 

  • Kozyrenko M.M., Artyukova E.V., Boltenkov E.V. & Lauve L.S. 2004. Somaklonal’naya izmenchivost’ Iris pseudacorus L. po dannym RAPD- i tsitogeneticheskogo analiza. Russ. J. Biotechnol. 20 (2): 13–23.

    Google Scholar 

  • Kucherevskyi V.V. 2001. Atlas ridkisnykh ta znykayuchykh roslin Dnipropetrovshchyny, Phytosociocentr, Kyiv, 360 pp.

    Google Scholar 

  • Kucherevskyi V.V. 2004. Konspekt flory Pravoberezhnoho stepovoho Prydniprov”ya, Prospect, Dnipropetrovsk, 292 pp.

    Google Scholar 

  • Leimu R., Mutikainen P., Koricheva J. & Fischer M. 2006. How general are positive relationships between plant population size, fitness and genetic variation? J. Ecol. 94: 942–952.

    Article  Google Scholar 

  • Hoskovec L. 2007. Zvláště chráněné druhy rostlin České republiky. https://doi.org/botany.cz/cs/chranene-rostliny/cs/chranene-rostliny/ (accessed 11.11. 2015).

    Google Scholar 

  • Eliáš jun. P. 2015. Červený zoznam výtrusných a kvitnúcich rastlín Slovenska. https://doi.org/botany.cz/cs/ohrozene-rastliny-slovenska//cs/ohrozene-rastliny-slovenska (accessed 11.11.2015).

    Google Scholar 

  • Lienert J. 2004. Habitat fragmentation effects on fitness of plant populations — a review. J. Nat. Conserv. 12: 53–72.

    Article  Google Scholar 

  • Makarevitch I., Golovnina K., Scherbik S. & Blinov A. 2003. Phylogenetic relationships of the Siberian Iris species inferred from noncoding chloroplast DNA sequences. Int. J. Plant Sci. 164: 229–237.

    Article  CAS  Google Scholar 

  • Mosyakin S.L. & Fedoronchuk M.M. 1999. Vascular plants of Ukraine: A nomenclatural checklist. M.G. Kholodny Institute of Botany, Kyiv, 346 pp.

    Google Scholar 

  • Motyka J., 1946. Step środkowo-europejski. Acta Soc. Bot. Pol. 17: 46.

    Google Scholar 

  • Ostapko V.M. & Kolomyichuk V.P. 2012. Krasnaya kniga Priazovskogo regiona. Sosudistie rasteniya. Altapress, Kyiv, 267 pp.

    Google Scholar 

  • Ostapko V.M. 2001. Raritetnyi florofond Yugo-Vostoka Ukrainy (khorolohiya). Lybed, Donetsk, 121 pp.

    Google Scholar 

  • Parnikoza I. 2008. Zberezhennya ukrayins’koho stepu: shcho mozhna zrobiti vzhe s’ohodni? pp. 53–62. In: Zagorodniuk I.V. (ed.), Rare theriofauna and its conservation, 9, Luhansk.

    Google Scholar 

  • Parnikoza I. 2011. Deyaki refuhiumy stepovoyi bioty ta mistsezrostannya ridkisnikh roslin v okolytsyakh mist Kryvy Rih ta Dnipropetrovs’k, pp. 31–40. In: Naukovi pratsi Luhans’koho prirodnoho zapovidnika. Roslinnii ta tvarinnii svit ta ioho okhorona. The flora and fauna, and conservation], Issue 2, Luhansk.

    Google Scholar 

  • Parnikoza I.Yu., Shevchenko N.M., Bogomaz M.V. & Shevchenko M.S. 2007. The influence of grass burning on the dynamics of Pulsatilla nigricans Stork population of the regional landscape park “Lysa Gora” (Kyiv), pp. 4–5. In: Szhmalej S.A., Rusina L.Yu., Akimov I.A. (eds), Falz-Fein Readings. PP Vyshemyrskyi, Kherson.

    Google Scholar 

  • Parnikoza I.Yu., Shevchenko M.S., Inozemtseva D.M., Vasyliuk O.V. & Shevchenko O.S. 2008. Raritetna flora (Okhorona, vivchennya, reintroduktsiya). Kyiv ecological and cultural centre, Kyiv, 132 pp.

    Google Scholar 

  • Peakall R. & Smouse P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. 6: 288–295.

    Article  Google Scholar 

  • Poradniki ochrony siedlisk i gatunków. Natura 2000 — podrecznik metodyczny, Murawy kserotermiczne. T. 3. 2004. Warszawa, 244 pp. https://doi.org/natura2000.gdos.gov.pl/uploads/download/219uploads/download/219 (accessed 31.07.2016)

    Google Scholar 

  • Purger D., Csiky J., Topić J. 2008. Dwarf iris, Iris pumila L. (Iridaceae), a new species of the Croatian flora. Acta Bot. Croat. 67: 97–102.

    Google Scholar 

  • Safriel N.U., Volis S. & Kark S. 1994. Core and peripheral populations and global climate change. Isr. J. Plant Sci. 42: 331–345.

    Article  Google Scholar 

  • Schluter P.M. & Harris S.A. 2006. Analysis of multilocus fingerprinting data sets containing missing data. Mol. Ecol. Notes. 6: 569–572.

    Article  CAS  Google Scholar 

  • Shaw J., Lickey E.B., Schilling E.E. & Small R.L. 2007. Comparison of whole chloroplast genome sequences to choose non-coding regions for phylogenetic studies in angiosperms: The tortoise and the hare III. Am. J. Bot. 94: 275–288.

    Article  CAS  PubMed  Google Scholar 

  • Shelegeda V.I. & Shelegeda O.R. 2008. Ridkisni ta znikayuchi roslini Zaporiz’koyi oblasti. Tandem-art-studia, Zaporizhzhia, 96 pp.

    Google Scholar 

  • Shvets T.A. 2004. Osoblivosti ontohenezu predstavnikiv riznikh sektsii rodu Iris L. v umovakh kul’turi. Visnik Lvivskoho universitetu. Seriia biolohichna. 36: 203–206.

    Google Scholar 

  • Sikura I.I. & Shisha E.N. 2008. Genus Iris L. (Iridaceae). Znania Ukrainy, Kiev, 195 pp.

    Google Scholar 

  • Sluginova I.S. 2008. Semennaya produktyvnost’ nekotorikh vidov rasteniĭ melovikh obnazheniĭ basseĭna Polnoĭ. Izvestiia vuzov. Severo-Kavkazskiĭ region. Estestv. Nauki. 146: 75–79.

    Google Scholar 

  • Taberlet P., Gielly L., Pautou G. & Bouvet J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17: 1105–1109.

    Article  CAS  PubMed  Google Scholar 

  • Tarasjev A., Klicaric N.B., Stojkovic B. & Avramov S. 2009. Phenotypic plasticity and between population differentiation in Iris pumila transplants between native open and anthropogenic shade habitats. Russ. J. Genet. 45: 1078–1086.

    Article  CAS  Google Scholar 

  • Tarasov V.V. 2005. Flora Dnipropetrovs’koyi ta Zaporiz’koyi oblastei. Sudinni roslini. Bioloho-ekolohichna kharakteristika vidiv: Monohrafiya. Publ. House of DNU, Dnipropetrovsk, 276 pp.

    Google Scholar 

  • Templeton A.R., Crandall K.A. & Sing C.F. 1992. A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics. 132: 619–633.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Tkachenko V.S., Didukh Ya.P., Henov A.P. et al. 1998. Ukrayins’kiĭ pryrodniĭ stepoviĭ zapovidnik. Roslynniĭ svit. Fitosotsiotsentr, Kyiv, 232 pp.

    Google Scholar 

  • Travlieiev A.P. 2010. Chervona Kniha Dinpropetrovs’koyi oblasti (Roslynniĭ svit). VKK “Blans-Klub”, Dnipropetrovsk, 500 p.

    Google Scholar 

  • Vedenkov E.P. 1989. Flora zapovidnika “Askaniya Nova”. Moscow, 50 pp.

    Google Scholar 

  • Wang K., Kang J., Zhou H., Sun Y., Yang Q., Dong J. & Meng L. 2009. Genetic diversity of Iris lactea var. chinensis germplasm detected by inter-simple sequence repeat (ISSR). Afr. J. Biotechnol. 8: 4856–4863.

    CAS  Google Scholar 

  • World checklist of selected plant families. https://doi.org/apps.kew.org/wcsp/qsearch.dowcsp/qsearch.do (accessed 11.11.2015).

  • Wróblewska A., Brzosko E., Czarnecka B. Nowosielski J. 2003. High levels of genetic diversity in populations of Iris aphylla L. (Iridaceae), an endangered species in Poland. Bot. J. Linn. Soc. 142: 65–72.

    Article  Google Scholar 

  • Wróblewska A. & Brzosko E. 2006. The genetic structure of the steppe plant Iris aphylla L. at the northern limit of its geographical range. Bot. J. Linn. Soc. 152: 245–255.

    Article  Google Scholar 

  • Yena A.V. 2012. Prirodnaya flora Krymskogo poluostrova. N. Oreanda, Simpheropol, 232 pp.

    Google Scholar 

  • Xie Z., Lu Y., Ge Song, Hong D and Li F. 2001. Clonality in wild rice (Oryza rufipogon, Poaceae) and its implications for conservation management. Am. J. Bot. 88: 1058–1064.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology and Genetics NAS of Ukraine, Acad. Zabolotnogo Str. 150, 03143, Kyiv, Ukraine

    Ivan Yu. Parnikoza, Igor O. Andreev, Olena M. Bublyk, Kateryna V. Spiridonova & Viktor A. Kunakh

  2. Department of Plant Taxonomy and Nature Conservation, University of Gdańsk, Wita Stwosza Str. 59, PL-80-308, Gdańsk, Poland

    Joanna Gołębiewska, Magdalena Kubiak, Anna Kuczyńska, Katarzyna Mystkowska, Natalia Olędrzyńska & Anna Ślęzak-Parnikoza

  3. Department of Molecular Evolution, University of Gdańsk, Wita Stwosza Str. 59, PL-80-308, Gdańsk, Poland

    Barbara Urasińska & Marcin Górniak

  4. Administration of Regional landscape parks of Lublin Vojevodship, Graniczna Str. 4, 20-010, Lublin, Poland

    Krzysztof Wojciechowski

  5. M. Kholodny Institute of Botany NAS of Ukraine, Tereshchenkivska Street 2, 01601, Kyiv, Ukraine

    Yakiv P. Didukh

Authors
  1. Ivan Yu. Parnikoza
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Igor O. Andreev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Olena M. Bublyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kateryna V. Spiridonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Joanna Gołębiewska
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Magdalena Kubiak
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Anna Kuczyńska
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Katarzyna Mystkowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Natalia Olędrzyńska
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Barbara Urasińska
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Anna Ślęzak-Parnikoza
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Marcin Górniak
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Krzysztof Wojciechowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Yakiv P. Didukh
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Viktor A. Kunakh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ivan Yu. Parnikoza.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Parnikoza, I.Y., Andreev, I.O., Bublyk, O.M. et al. The current state of steppe perennial plants populations: A case study on Iris pumila. Biologia 72, 24–35 (2017). https://doi.org/10.1515/biolog-2017-0002

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1515/biolog-2017-0002

Key words

Search

Navigation