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Insulin-like growth factor binding proteins in the bovine anterior pituitary

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Abstract

Insulin-like growth factor binding proteins (IGFBPs) were characterized in bovine anterior pituitary tissue, pituitary conditioned media, and serum collected during the preovulatory and early luteal phases of the estrous cycle. Effects of in vitro treatments of pituitaries with luteinizing hormone-releasing hormone (LHRH), estradiol, and progesterone on IGFBP secretion were also evaluated. Predominant IGFBPs detected in anterior pituitary tissue by immunoprecipitation, ligand blotting, and Northern blotting were IGFBP-5 (29 kDa), IGFBP-2 (32 kDa), and IGFBP-3 (36 and 39 kDa doublet). Conditioned culture media contained IGFBP-5, a slightly larger form of IGFBP-3 (33 kDa), the 36- and 39-kDa forms of IGFBP-3, and a more extensively glycosylated form of IGFBP-3 (44 kDa). In serum, IGFBP-5 was not readily detected, and IGFBP-3 (40- and 44-kDa doublet) and IGFBP-2 (34 kDa) were larger than in pituitary tissue. Levels of IGFBP-2,-3, and-5 in pituitary tissue decreased during the preovulatory period and were lowest in the early luteal phase. Treatment with LHRH increased IGFBP-2 levels in media twofold. Estradiol or progesterone did not alter IGFBP secretion in vitro. Predominant IGFBPs produced and released by anterior pituitary tissue were IGFBP-2,-3 and-5. The activity of IGFBPs fluctuates in the pituitary in association with changes in stage of estrous cycle, implicating IGFBPs as potential regulators of gonadotrope function.

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Author notes

  1. Richard N. Funston

    Present address: Extension Service, USDA, ARS Fort Keogh LARRL, Montana State University, 59301, Miles City, MT

Authors and Affiliations

  1. USDA-ARS, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE

    Andrew J. Roberts

  2. Department of Animal Science, University of Wyoming, Laramie, WY

    Richard N. Funston & Gary E. Moss

Authors
  1. Andrew J. Roberts
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  2. Richard N. Funston
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  3. Gary E. Moss
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Correspondence to Andrew J. Roberts.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Roberts, A.J., Funston, R.N. & Moss, G.E. Insulin-like growth factor binding proteins in the bovine anterior pituitary. Endocr 14, 399–406 (2001). https://doi.org/10.1385/ENDO:14:3:399

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  • DOI: https://doi.org/10.1385/ENDO:14:3:399

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