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Insulin-like growth factor 1 (IGF-1) regulates prolactin, growth hormone, and IGF-1 receptor expression in the pituitary gland of the gilthead sea bream Sparus aurata

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Abstract

The role of insulin-like growth factor 1 (IGF-1) on regulation of growth hormone (GH) and prolactin (PRL) as well as the possible involvement of IGF-1 receptor subtype a (IGF-1Ra) mRNA was assessed in juvenile specimens of Sparus aurata. IGF-1Ra was successfully cloned, and active receptor domains were localized in its mRNA precursor. Also, phylogenetic analysis of the protein sequence indicated a closer proximity to IGF-1Ra isoform found in zebrafish and other teleosts, than to the isoform IGF-1Rb. The most abundant presence of IGF-1Ra mRNA was detected in white muscle, whereas head kidney showed the lowest gene expression among 24 different studied tissues. Pituitaries of juvenile specimens of S. aurata were incubated in vitro with different doses of IGF-1 (0, 1, 100, and 1000 ng mL−1) during a period of 10 h. Total RNA with a high quality could be obtained from these pituitaries. PRL mRNA expression significantly increased with increasing IGF-1 doses. Similarly, IGF-1Ra mRNA increased its expression in response to IGF-1. However, GH mRNA levels decreased in a dose-dependent manner after IGF-1 treatment. The contradictory responses of GH and PRL expressions to IGF-1 in our experiment are possibly mediated by IGF-1Ra presence on the somatotrophs and prolactotrophs. The increase in IGF-1Ra mRNA levels may be related to the proper activation of the PI3-K/Akt signal transduction pathways which are normally involved in GH and PRL regulation.

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Acknowledgments

The authors would like to appreciate their thanks to Prof. Juan Fuentes (Centre of Marine Sciences, University of Algarve, Faro, Portugal) for his generous and comprehensive guidance during the fulfillment of this work. This work has been done in the Campus of Excellence on the Sea (CEI-MAR).

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  1. Departamento de Biología Marina y Acuicultura, Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), 11510, Puerto Real, Cádiz, Spain

    Khaled Mohammed-Geba, J. A. Martos-Sitcha, A. Galal-Khallaf & G. Martínez-Rodríguez

  2. Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Campus de Excelencia Internacional del Mar (CEIMAR), 11510, Puerto Real, Cádiz, Spain

    Khaled Mohammed-Geba, J. A. Martos-Sitcha, A. Galal-Khallaf & J. M. Mancera

  3. Genetic Engineering and Molecular Biology Division, Department of Zoology, Faculty of Sciences, Menoufia University, Shebin El-Kom, Menoufia, Egypt

    Khaled Mohammed-Geba & A. Galal-Khallaf

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  1. Khaled Mohammed-Geba
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  2. J. A. Martos-Sitcha
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Correspondence to Khaled Mohammed-Geba.

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Mohammed-Geba, K., Martos-Sitcha, J.A., Galal-Khallaf, A. et al. Insulin-like growth factor 1 (IGF-1) regulates prolactin, growth hormone, and IGF-1 receptor expression in the pituitary gland of the gilthead sea bream Sparus aurata . Fish Physiol Biochem 42, 365–377 (2016). https://doi.org/10.1007/s10695-015-0144-8

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