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Application of factorial design to the study of xylitol production from eucalyptus hemicellulosic hydrolysate

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Abstract

This study deals with the bioconversion of xylose into xylitol by Candida guilliermondii FTI 20037 using eucalyptus hemicellulosic hydrolysate obtained by acid hydrolysis. The influence of various parameters (ammonium sulfate, rice bran, pH, and xylose concentration) on the production of xylitol was evaluated. The experiments were based on multivariate statistical concepts, with the application of factorial design techniques to identify the most important variables in the process. The levels of these variables were quantified by the response surface methodology, which permitted the establishment of a significant mathematical model with a coefficient determination of R 2=0.92. The best results (xylitol=10.0 g/L, yield factor=0.2 g/g, and productivity=0.1 g/[L·h]) were attained with hydrolysate containing ammonium sulfate (1.1 g/L), rice bran (5.0 g/L), and xylose (initial concentration of 60.0 g/L), after 72 h of fermentation. The pH of fermentation was adjusted to 8.0 and the inoculum level utilized was 3 g/L.

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Authors and Affiliations

  1. Department of Biotechnology, FAENQUIL, Rod. Itajubá-Lorena Km 74.5, PO Box 116, 12600-000, Lorena, SP, Brazil

    Eliana V. Canettieri, João B. Almeida E. Silva & Maria G. A. Felipe

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  1. Eliana V. Canettieri
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  2. João B. Almeida E. Silva
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  3. Maria G. A. Felipe
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Correspondence to Eliana V. Canettieri.

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Canettieri, E.V., Almeida E. Silva, J.B. & Felipe, M.G.A. Application of factorial design to the study of xylitol production from eucalyptus hemicellulosic hydrolysate. Appl Biochem Biotechnol 94, 159–168 (2001). https://doi.org/10.1385/ABAB:94:2:159

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  • DOI: https://doi.org/10.1385/ABAB:94:2:159

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