Skip to main content
SpringerLink
Log in

Phosphodiesterase production in an aqueous two-phase system by Nicotiana tabacum 1507

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Studies were conducted on the production of phosphodiesterases by Nicotiana tabacum 1507 cell suspension in an aqueous two-phase system formed by adding 4% polyethylene glycol (mol wt 20,000) and 7.5% dextran (mol wt 70,000) to the medium. The time course of growth, biosynthesis, secretion, and partitioning of phosphodiesterases was followed in comparison with N. tabacum 1507 cultivation as a free suspension. Partitioning of phosphodiesterases took place mainly in the bottom dextran phase, and a possibility was revealed for obtaining an enzyme preparation with high phosphodiesterase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Albertsson, P. A. (1986), Partition of Cell Particles and Macromolecules, 3rd ed., Wiley, New York.

    Google Scholar 

  2. Andrews, B. A. and Asenjo, J. A. (1989), in Protein Purification Methods: A Practical Approach, Harris, E. L. V. and Angal, S., eds., IRL, Oxford, pp. 161–174.

    Google Scholar 

  3. Grosmann, C., Tintiger, R., Zhu, J., and Mauzer, G. (1997), Fluid Phase Equilibria 137, 209–228.

    Article  Google Scholar 

  4. Carlson, A. (1988), Sep. Sci. Technol. 23, 785–817.

    Article  CAS  Google Scholar 

  5. Schmidt, A. S., Ventom, A. M., and Asenjo, J. A. (1994), Enzyme Microbiol. Technol. 16, 131–142.

    Article  CAS  Google Scholar 

  6. Andrews, B. A., Nielson, S., and Asenjo, J. A. (1996), Bioseparation 6, 303–313.

    CAS  Google Scholar 

  7. Cascone, O. B. A., Andrews, B. A., and Asenjo, J. A. (1991), Enzyme Microbiol. Technol. 31, 629–635.

    Article  Google Scholar 

  8. Kula, M.-R. (1990), Bioseparation 1, 181–189.

    CAS  Google Scholar 

  9. Kuboi, R., Umakoshi, H., and Komasawa, I. (1995), Biotechnol. Prog. 11, 202–207.

    Article  CAS  Google Scholar 

  10. Kim, S. H. and Yoo, Y. J. (1991), J. Ferment. Bioeng. 71, 373–375.

    Article  CAS  Google Scholar 

  11. Choi, J. S. and Yoo, Y. J. (1988), K. J. Appl. Microbiol. Bioeng. 16, 358–362.

    CAS  Google Scholar 

  12. Andersson, E. A., Johansson, C., and Hahn-Haengerdal, B. (1985), Enzyme Microbiol. Technol. 7, 333–338.

    Article  CAS  Google Scholar 

  13. Chen, J. P. and Lee, M. S. (1995), Enzyme Microbiol. Technol. 17, 1021–1027.

    Article  CAS  Google Scholar 

  14. Persson, I., Staalbrand, H., Tjerneld, F., and Hahn-Hagerdal, B. (1992), Appl. Biochem. Biotechnol. 27, 27–36.

    CAS  Google Scholar 

  15. Persson, I., Tjerneld, F., and Hahn-Hagerdal, B. (1992), Appl. Biochem. Biotechnol. 27, 9–25.

    Article  CAS  Google Scholar 

  16. Zijlstrea, G. M., de Gooijer, C. D., and Tramper, J. (1998), Curr. Opin. Biotechnol. 9, 171–176.

    Article  Google Scholar 

  17. Hooker, B. S. and Lee, J. M. (1990), Plant Cell Rep. 8, 546–549.

    Article  CAS  Google Scholar 

  18. Ilieva, M. P., Bakalova, A., Mihneva, M., Pavlov, A., and Dolapchiev, L. (1996), Biotechnol. Bioeng. 51, 488–493.

    Article  CAS  Google Scholar 

  19. Buitelaar, R. M., Leener, E. J. T. M., and Tramper, J. (1992), Biocatalysis 6, 73–80.

    Article  CAS  Google Scholar 

  20. Linsmayer, E. M. and Skoog, F. (1965), Physiol. Plantarum 18, 100–127.

    Article  Google Scholar 

  21. Ilieva, M., Bakalova, A., Kojuharova, A., Pavlov, A., and Mihneva, M. (1995), Comp. Rend. Bulg. Acad. Sci. 48(6), 69–72.

    CAS  Google Scholar 

  22. Ilieva, M., Kojuharova, A., Pavlov, A., Mihneva, M., and Shterev, I. (1995), Biotechnol. Biotechnol. Eq. 9, 71–76.

    Google Scholar 

  23. Dixon, R. A. (1985), in Plant Cell Culture—A Practical Approach, Dixon, R. A., ed., IRL, Oxford, pp. 1–20.

    Google Scholar 

  24. Dolaptchiev, L. (1980), Int. J. Biol. Macromol. 2, 7–12.

    Article  Google Scholar 

  25. Ogata, K. (1975), in Advances in Applied Microbiology, vol. 19, Perlman, P., ed., Academic, New York, pp. 209–247.

    Google Scholar 

  26. Misawa, M. (1977), in Plant Cell Tissue Culture and Its Biotechnological Application, Barz, W., Reinhard, E., and Zenk, M. H., eds., Springer-Verlag, New York, pp. 17–26.

    Google Scholar 

  27. Dolaptchiev, L. B., Bakalova, A. T., Vassileva, R. A., Ilieva, M. P., and Mihneva, M. D. (1996), Enzyme Microbiol. Technol. 19, 384–388.

    Article  Google Scholar 

  28. Wink, M. (1994), Plant Cell Tissue Organ Culture 38, 307–319.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, Laboratory of Biotechnology and Industrial Microbiology, Bulgarian Academy of Sciences, 26 Maritza Boulevard, 4002, Plovdiv, Bulgaria

    Mladenka Ilieva

  2. Bulgarian Academy of Sciences, Institute of Molecular Biology, 1113, Sofia, Bulgaria

    Atanas Pavlov & Anastasia Bacalova

Authors
  1. Mladenka Ilieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Atanas Pavlov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anastasia Bacalova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mladenka Ilieva.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ilieva, M., Pavlov, A. & Bacalova, A. Phosphodiesterase production in an aqueous two-phase system by Nicotiana tabacum 1507. Appl Biochem Biotechnol 90, 261–272 (2001). https://doi.org/10.1385/ABAB:90:3:261

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:90:3:261

Index Entries

Search

Navigation